ENSG00000189403

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000339872 ENSG00000189403 HEK293_OSMI2_2hA HEK293_TMG_2hB HMGB1 protein_coding protein_coding 489.5102 335.8195 757.2758 41.49018 15.05474 1.173109 89.10437 42.79315 160.51500 8.609953 7.364376 1.907009 0.1596375 0.1252000 0.2118667 0.08666667 2.753686e-05 1.275099e-39 FALSE TRUE
ENST00000399494 ENSG00000189403 HEK293_OSMI2_2hA HEK293_TMG_2hB HMGB1 protein_coding protein_coding 489.5102 335.8195 757.2758 41.49018 15.05474 1.173109 60.52307 95.76512 24.19561 15.847324 3.029351 -1.984310 0.1691458 0.2830000 0.0318000 -0.25120000 1.275099e-39 1.275099e-39 FALSE TRUE
MSTRG.8550.6 ENSG00000189403 HEK293_OSMI2_2hA HEK293_TMG_2hB HMGB1 protein_coding   489.5102 335.8195 757.2758 41.49018 15.05474 1.173109 304.14021 187.33644 499.88404 16.998722 12.061485 1.415914 0.6067583 0.5621667 0.6600333 0.09786667 9.738867e-03 1.275099e-39 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000189403 E001 171.1602686 0.0020319153 7.139290e-22 5.598167e-20 13 30456704 30458746 2043 - 2.325 1.957 -1.231
ENSG00000189403 E002 464.4049607 0.0009934410 4.302259e-58 4.116414e-55 13 30458747 30459849 1103 - 2.763 2.369 -1.314
ENSG00000189403 E003 1925.5794312 0.0040991260 5.948855e-10 9.202194e-09 13 30459850 30460894 1045 - 3.328 3.148 -0.598
ENSG00000189403 E004 176.2282193 0.0042489262 2.147289e-01 3.441134e-01 13 30460895 30460906 12 - 2.253 2.208 -0.150
ENSG00000189403 E005 1028.6204619 0.0020547917 6.245219e-10 9.629185e-09 13 30460907 30461036 130 - 2.936 3.090 0.511
ENSG00000189403 E006 4119.8593300 0.0011584134 4.085576e-13 1.065822e-11 13 30461037 30461495 459 - 3.557 3.667 0.367
ENSG00000189403 E007 113.3866648 0.0003546245 4.606221e-05 2.618348e-04 13 30461496 30461533 38 - 1.987 2.139 0.508
ENSG00000189403 E008 48.7687462 0.0131303117 5.323699e-02 1.149060e-01 13 30461534 30461638 105 - 1.728 1.581 -0.498
ENSG00000189403 E009 45.3437008 0.0048292098 4.352550e-02 9.751160e-02 13 30461639 30461688 50 - 1.692 1.569 -0.418
ENSG00000189403 E010 103.8931089 0.0002549456 2.742782e-31 5.015559e-29 13 30462346 30462537 192 - 2.131 1.644 -1.640
ENSG00000189403 E011 2392.9418931 0.0001103523 7.664286e-01 8.489201e-01 13 30462538 30462712 175 - 3.363 3.376 0.044
ENSG00000189403 E012 3806.9940182 0.0001349510 9.648006e-01 9.818096e-01 13 30463207 30463310 104 - 3.565 3.574 0.031
ENSG00000189403 E013 3136.9886774 0.0002192385 4.778606e-02 1.052384e-01 13 30463311 30463352 42 - 3.474 3.500 0.084
ENSG00000189403 E014 15.6276610 0.0187943025 3.133465e-01 4.559231e-01 13 30463353 30463530 178 - 1.244 1.134 -0.388
ENSG00000189403 E015 3461.7248885 0.0009587352 5.505187e-01 6.801362e-01 13 30463531 30463694 164 - 3.527 3.521 -0.022
ENSG00000189403 E016 66.0217709 0.0194330551 1.552663e-02 4.168637e-02 13 30463695 30464160 466 - 1.726 1.921 0.657
ENSG00000189403 E017 30.2827761 0.0229409507 4.377238e-02 9.797315e-02 13 30464161 30464310 150 - 1.393 1.590 0.675
ENSG00000189403 E018 0.1482932 0.0411597534 2.412089e-01   13 30465073 30465242 170 - 0.000 0.167 9.297
ENSG00000189403 E019 1344.8859631 0.0044660197 1.357020e-01 2.420859e-01 13 30465796 30465953 158 - 3.133 3.080 -0.176
ENSG00000189403 E020 12.0514959 0.0014621590 5.075136e-01 6.430000e-01 13 30616671 30617597 927 - 1.074 1.150 0.273