ENSG00000189114

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000433642 ENSG00000189114 HEK293_OSMI2_2hA HEK293_TMG_2hB BLOC1S3 protein_coding protein_coding 18.44739 33.79341 10.45878 0.395055 0.2814811 -1.691076 11.863130 18.709170 8.0902175 1.3424614 0.2686937 -1.208484 0.66866667 0.55343333 0.77326667 0.21983333 5.945513e-05 5.945513e-05 FALSE TRUE
ENST00000587722 ENSG00000189114 HEK293_OSMI2_2hA HEK293_TMG_2hB BLOC1S3 protein_coding protein_coding 18.44739 33.79341 10.45878 0.395055 0.2814811 -1.691076 1.402321 2.327906 1.2103988 0.5486815 0.2070948 -0.937864 0.08075833 0.06873333 0.11690000 0.04816667 4.113664e-01 5.945513e-05 FALSE TRUE
ENST00000588362 ENSG00000189114 HEK293_OSMI2_2hA HEK293_TMG_2hB BLOC1S3 protein_coding processed_transcript 18.44739 33.79341 10.45878 0.395055 0.2814811 -1.691076 3.670212 10.267680 0.3375862 2.1200371 0.3375862 -4.886000 0.17655000 0.30426667 0.03066667 -0.27360000 3.928305e-02 5.945513e-05   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000189114 E001 1.5791871 0.0255739484 6.046601e-02 1.274134e-01 19 45178555 45178721 167 + 0.000 0.406 11.784
ENSG00000189114 E002 1.1760376 0.0124395760 1.194647e-01 2.191835e-01 19 45178722 45178741 20 + 0.000 0.334 13.429
ENSG00000189114 E003 14.8551282 0.0024950970 7.755095e-01 8.553726e-01 19 45178742 45178783 42 + 1.136 1.112 -0.085
ENSG00000189114 E004 47.3473799 0.0028895149 1.613500e-02 4.303189e-02 19 45178784 45178831 48 + 1.704 1.565 -0.473
ENSG00000189114 E005 35.7686342 0.0010261677 4.972623e-01 6.338084e-01 19 45179223 45179287 65 + 1.510 1.470 -0.139
ENSG00000189114 E006 110.4126122 0.0148597263 6.492631e-03 1.990307e-02 19 45179288 45179668 381 + 2.089 1.922 -0.558
ENSG00000189114 E007 33.5779168 0.0031503204 3.867813e-06 2.823682e-05 19 45179669 45179821 153 + 1.669 1.366 -1.041
ENSG00000189114 E008 54.2373763 0.0006407280 1.861967e-02 4.849782e-02 19 45179822 45179913 92 + 1.749 1.629 -0.405
ENSG00000189114 E009 49.0921494 0.0018474076 1.698607e-01 2.880302e-01 19 45179914 45179942 29 + 1.674 1.599 -0.256
ENSG00000189114 E010 136.6029257 0.0033982865 1.704096e-01 2.886975e-01 19 45179943 45180192 250 + 2.095 2.044 -0.170
ENSG00000189114 E011 93.3782638 0.0003564112 8.886892e-01 9.328217e-01 19 45180193 45180267 75 + 1.896 1.895 -0.002
ENSG00000189114 E012 134.0233116 0.0024200106 1.171280e-01 2.158429e-01 19 45180268 45180413 146 + 1.993 2.065 0.241
ENSG00000189114 E013 508.4444650 0.0015112810 9.811410e-01 9.922354e-01 19 45180414 45181271 858 + 2.621 2.628 0.022
ENSG00000189114 E014 197.7674323 0.0012247215 1.149800e-02 3.234434e-02 19 45181272 45181361 90 + 2.145 2.235 0.299
ENSG00000189114 E015 387.0825784 0.0036419908 5.200633e-04 2.234976e-03 19 45181362 45181800 439 + 2.418 2.533 0.386
ENSG00000189114 E016 0.2965864 0.2266751568 7.694720e-01   19 45187331 45187387 57 + 0.000 0.139 10.817
ENSG00000189114 E017 0.0000000       19 45187388 45187441 54 +      
ENSG00000189114 E018 0.0000000       19 45187587 45187614 28 +      
ENSG00000189114 E019 0.6663248 0.0811885776 2.881197e-01 4.286599e-01 19 45187615 45187740 126 + 0.000 0.246 12.248
ENSG00000189114 E020 0.0000000       19 45198889 45199013 125 +      
ENSG00000189114 E021 1.0298353 0.0121989077 6.793294e-01 7.835428e-01 19 45202406 45202507 102 + 0.322 0.248 -0.517
ENSG00000189114 E022 0.8470867 0.0143775777 1.097169e-01 2.051434e-01 19 45216676 45216933 258 + 0.424 0.141 -2.101