ENSG00000188846

Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000435633 ENSG00000188846 HEK293_OSMI2_2hA HEK293_TMG_2hB RPL14 protein_coding nonsense_mediated_decay 993.8784 1374.027 841.7206 165.0536 49.60223 -0.7069907 785.7664 1303.55073 543.9731 155.0706 27.74469 -1.260824 0.7684542 0.94973333 0.6514000 -0.2983333 0.003053223 3.922971e-06 FALSE TRUE
ENST00000461368 ENSG00000188846 HEK293_OSMI2_2hA HEK293_TMG_2hB RPL14 protein_coding processed_transcript 993.8784 1374.027 841.7206 165.0536 49.60223 -0.7069907 190.5440 56.59532 271.7395 30.2567 62.97282 2.263268 0.2125625 0.04003333 0.3174333 0.2774000 0.324303392 3.922971e-06   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.