ENSG00000188827

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000294008 ENSG00000188827 HEK293_OSMI2_2hA HEK293_TMG_2hB SLX4 protein_coding protein_coding 5.188429 5.58784 4.094707 0.4722181 0.07876569 -0.4475908 4.6227905 5.2992852 3.5936064 0.47524825 0.03181375 -0.5590762 0.88841667 0.94763333 0.8783667 -0.06926667 6.461931e-02 4.974411e-09 FALSE TRUE
ENST00000466154 ENSG00000188827 HEK293_OSMI2_2hA HEK293_TMG_2hB SLX4 protein_coding retained_intron 5.188429 5.58784 4.094707 0.4722181 0.07876569 -0.4475908 0.3514157 0.0000000 0.5011009 0.00000000 0.09154579 5.6755364 0.07027500 0.00000000 0.1216333 0.12163333 4.974411e-09 4.974411e-09 FALSE TRUE
ENST00000486524 ENSG00000188827 HEK293_OSMI2_2hA HEK293_TMG_2hB SLX4 protein_coding retained_intron 5.188429 5.58784 4.094707 0.4722181 0.07876569 -0.4475908 0.2142225 0.2885544 0.0000000 0.04085131 0.00000000 -4.8999220 0.04130417 0.05236667 0.0000000 -0.05236667 5.100198e-05 4.974411e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000188827 E001 283.3926778 0.0115207635 3.075482e-10 4.995635e-09 16 3581181 3582693 1513 - 2.186 2.535 1.164
ENSG00000188827 E002 114.1906027 0.0025735339 2.995269e-03 1.024990e-02 16 3583097 3583510 414 - 1.932 2.097 0.551
ENSG00000188827 E003 42.7581676 0.0135618982 8.781527e-02 1.718080e-01 16 3584769 3584871 103 - 1.502 1.682 0.612
ENSG00000188827 E004 321.8746248 0.0007100030 1.633786e-01 2.795671e-01 16 3589002 3591310 2309 - 2.480 2.497 0.056
ENSG00000188827 E005 46.7650578 0.0005630740 1.822581e-03 6.672125e-03 16 3592699 3592865 167 - 1.729 1.612 -0.397
ENSG00000188827 E006 30.3070088 0.0069318781 9.095967e-03 2.652184e-02 16 3594453 3594599 147 - 1.562 1.417 -0.498
ENSG00000188827 E007 11.4363663 0.0016617843 2.369475e-01 3.706419e-01 16 3595605 3595610 6 - 1.129 1.054 -0.272
ENSG00000188827 E008 22.1427573 0.0157790529 2.236498e-01 3.547608e-01 16 3595611 3595693 83 - 1.394 1.328 -0.229
ENSG00000188827 E009 35.6580534 0.0132475024 8.819009e-01 9.283304e-01 16 3596153 3596393 241 - 1.523 1.561 0.131
ENSG00000188827 E010 49.5449916 0.0044481707 3.465048e-01 4.906260e-01 16 3597379 3597695 317 - 1.690 1.679 -0.037
ENSG00000188827 E011 40.2425123 0.0026836202 2.091926e-02 5.342028e-02 16 3597797 3597999 203 - 1.650 1.555 -0.322
ENSG00000188827 E012 0.5514428 0.0196730038 1.797542e-01 3.008506e-01 16 3600621 3600978 358 - 0.000 0.259 9.671
ENSG00000188827 E013 42.3588613 0.0008239029 3.741030e-02 8.624185e-02 16 3600979 3601191 213 - 1.657 1.586 -0.243
ENSG00000188827 E014 18.5209488 0.0255264428 4.696127e-02 1.037573e-01 16 3601192 3602117 926 - 1.374 1.198 -0.615
ENSG00000188827 E015 36.1997000 0.0008890693 1.793835e-03 6.579988e-03 16 3602118 3602307 190 - 1.630 1.490 -0.479
ENSG00000188827 E016 37.3303595 0.0005750046 9.448724e-07 7.879358e-06 16 3606474 3606698 225 - 1.696 1.462 -0.799
ENSG00000188827 E017 104.5053128 0.0102959013 4.256683e-06 3.075692e-05 16 3608430 3609566 1137 - 2.127 1.915 -0.710
ENSG00000188827 E018 5.8219361 0.0280668339 1.759742e-01 2.959623e-01 16 3611560 3611606 47 - 0.904 0.752 -0.595