ENSG00000188811

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000379599 ENSG00000188811 HEK293_OSMI2_2hA HEK293_TMG_2hB NHLRC3 protein_coding protein_coding 8.256294 5.152233 13.00374 0.5473374 0.3661358 1.333968 0.8642214 0.3231587 1.041905 0.0326127 0.12757585 1.6587231 0.1031542 0.06443333 0.08023333 0.01580000 0.70425271 0.02337112 FALSE TRUE
ENST00000379600 ENSG00000188811 HEK293_OSMI2_2hA HEK293_TMG_2hB NHLRC3 protein_coding protein_coding 8.256294 5.152233 13.00374 0.5473374 0.3661358 1.333968 2.7678353 2.1910410 4.145557 0.2432781 0.06537352 0.9168559 0.3416750 0.42490000 0.31906667 -0.10583333 0.02337112 0.02337112 FALSE TRUE
ENST00000473371 ENSG00000188811 HEK293_OSMI2_2hA HEK293_TMG_2hB NHLRC3 protein_coding retained_intron 8.256294 5.152233 13.00374 0.5473374 0.3661358 1.333968 1.1072173 0.4304758 1.761587 0.1050682 0.12962752 2.0079072 0.1245083 0.08126667 0.13623333 0.05496667 0.21022332 0.02337112 TRUE FALSE
ENST00000485407 ENSG00000188811 HEK293_OSMI2_2hA HEK293_TMG_2hB NHLRC3 protein_coding retained_intron 8.256294 5.152233 13.00374 0.5473374 0.3661358 1.333968 3.3059949 2.1324439 5.741896 0.2097301 0.59183139 1.4247801 0.4063417 0.41546667 0.44010000 0.02463333 0.86687405 0.02337112 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000188811 E001 0.1515154 0.0428818921 1.000000e+00   13 39038306 39038310 5 + 0.097 0.000 -7.907
ENSG00000188811 E002 14.6792042 0.0160685123 2.420331e-01 3.765898e-01 13 39038311 39038390 80 + 1.253 1.107 -0.521
ENSG00000188811 E003 30.9770475 0.0018926858 7.196637e-01 8.144207e-01 13 39038391 39038455 65 + 1.526 1.469 -0.195
ENSG00000188811 E004 26.8625561 0.0013727708 8.712693e-01 9.212053e-01 13 39038456 39038465 10 + 1.453 1.430 -0.077
ENSG00000188811 E005 100.6553600 0.0003899329 3.999882e-06 2.910211e-05 13 39038466 39038723 258 + 1.953 2.073 0.402
ENSG00000188811 E006 5.5139143 0.0798072225 2.944308e-01 4.355372e-01 13 39038724 39039135 412 + 0.888 0.647 -0.969
ENSG00000188811 E007 87.5191420 0.0004223790 2.434464e-07 2.295107e-06 13 39039136 39039288 153 + 1.877 2.029 0.509
ENSG00000188811 E008 94.8670115 0.0002857482 3.794531e-04 1.695190e-03 13 39039564 39039711 148 + 1.941 2.029 0.295
ENSG00000188811 E009 42.6852737 0.0005544791 8.069274e-14 2.326080e-12 13 39039712 39041817 2106 + 1.780 1.286 -1.694
ENSG00000188811 E010 4.3190891 0.0037186534 3.726495e-04 1.668179e-03 13 39042058 39042104 47 + 0.887 0.267 -2.980
ENSG00000188811 E011 90.1881431 0.0007709915 5.061942e-04 2.183496e-03 13 39042105 39042305 201 + 1.916 2.008 0.309
ENSG00000188811 E012 43.7017811 0.0010835173 4.309910e-01 5.735880e-01 13 39044090 39044181 92 + 1.644 1.651 0.026
ENSG00000188811 E013 36.5540750 0.0089811963 1.109765e-01 2.069755e-01 13 39044182 39044464 283 + 1.629 1.475 -0.526
ENSG00000188811 E014 19.4982706 0.0117251090 1.846115e-03 6.746675e-03 13 39047040 39047152 113 + 1.417 1.067 -1.237
ENSG00000188811 E015 158.8131437 0.0187575859 2.961567e-03 1.014905e-02 13 39047674 39050109 2436 + 2.274 2.060 -0.716