ENSG00000187961

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000338591 ENSG00000187961 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL17 protein_coding protein_coding 22.99166 18.27975 18.91164 3.425784 1.418041 0.04900147 0.1764014 1.082768 0.0000000 0.5843422 0.0000000 -6.7718430 0.00852500 0.0508000 0.00000000 -0.05080000 1.344221e-01 2.858352e-30 FALSE TRUE
ENST00000463212 ENSG00000187961 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL17 protein_coding retained_intron 22.99166 18.27975 18.91164 3.425784 1.418041 0.04900147 3.9456035 1.289498 4.6098614 0.6450827 0.6333708 1.8298951 0.16403333 0.0597000 0.24146667 0.18176667 2.801838e-01 2.858352e-30 FALSE TRUE
ENST00000466300 ENSG00000187961 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL17 protein_coding nonsense_mediated_decay 22.99166 18.27975 18.91164 3.425784 1.418041 0.04900147 1.9102164 0.000000 2.2500174 0.0000000 0.1095821 7.8201901 0.07953333 0.0000000 0.11963333 0.11963333 2.858352e-30 2.858352e-30 FALSE FALSE
ENST00000481067 ENSG00000187961 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL17 protein_coding retained_intron 22.99166 18.27975 18.91164 3.425784 1.418041 0.04900147 5.9554554 3.053837 4.1398747 1.5276383 0.5092470 0.4377281 0.25153750 0.1416333 0.21733333 0.07570000 7.542290e-01 2.858352e-30   FALSE
MSTRG.55.10 ENSG00000187961 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL17 protein_coding   22.99166 18.27975 18.91164 3.425784 1.418041 0.04900147 1.3491566 2.748164 0.6340106 0.2981433 0.3254403 -2.0985517 0.06550417 0.1561667 0.03496667 -0.12120000 2.602636e-01 2.858352e-30 FALSE TRUE
MSTRG.55.17 ENSG00000187961 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL17 protein_coding   22.99166 18.27975 18.91164 3.425784 1.418041 0.04900147 1.2397965 2.542339 0.2729152 0.1583419 0.2729152 -3.1733779 0.05796250 0.1540333 0.01696667 -0.13706667 5.648767e-02 2.858352e-30 TRUE TRUE
MSTRG.55.19 ENSG00000187961 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL17 protein_coding   22.99166 18.27975 18.91164 3.425784 1.418041 0.04900147 3.1493037 3.528692 2.3482361 0.2340779 0.3329738 -0.5855080 0.14031667 0.2053000 0.12393333 -0.08136667 1.620935e-01 2.858352e-30 FALSE TRUE
MSTRG.55.6 ENSG00000187961 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL17 protein_coding   22.99166 18.27975 18.91164 3.425784 1.418041 0.04900147 2.5884047 2.170613 2.9143216 0.5350191 0.1899890 0.4233684 0.11392500 0.1251333 0.15573333 0.03060000 7.102952e-01 2.858352e-30 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000187961 E001 0.000000       1 960094 960187 94 +      
ENSG00000187961 E002 0.000000       1 960188 960240 53 +      
ENSG00000187961 E003 0.000000       1 960241 960280 40 +      
ENSG00000187961 E004 0.000000       1 960281 960321 41 +      
ENSG00000187961 E005 0.000000       1 960322 960506 185 +      
ENSG00000187961 E006 2.022070 0.0239939040 1.344624e-01 2.403647e-01 1 960507 960583 77 + 0.590 0.338 -1.296
ENSG00000187961 E007 5.191048 0.0420650704 5.605560e-02 1.197976e-01 1 960584 960613 30 + 0.915 0.632 -1.140
ENSG00000187961 E008 14.846377 0.0229634707 2.575463e-03 8.990654e-03 1 960614 960800 187 + 1.342 1.023 -1.135
ENSG00000187961 E009 2.733474 0.0119277872 1.021519e-01 1.937834e-01 1 960801 960890 90 + 0.697 0.441 -1.179
ENSG00000187961 E010 1.949975 0.0073774176 3.363061e-01 4.801292e-01 1 960891 960892 2 + 0.548 0.392 -0.790
ENSG00000187961 E011 6.649320 0.0024427666 4.020078e-03 1.319802e-02 1 960893 960921 29 + 1.046 0.707 -1.305
ENSG00000187961 E012 13.476910 0.0295284214 2.820762e-05 1.688367e-04 1 961293 961303 11 + 1.376 0.876 -1.805
ENSG00000187961 E013 12.297758 0.0278513696 9.843047e-04 3.905451e-03 1 961304 961310 7 + 1.297 0.910 -1.399
ENSG00000187961 E014 14.207989 0.0313353632 8.962755e-03 2.618622e-02 1 961311 961320 10 + 1.320 1.022 -1.061
ENSG00000187961 E015 14.427269 0.0352212266 3.120300e-02 7.429449e-02 1 961321 961324 4 + 1.305 1.058 -0.879
ENSG00000187961 E016 28.140732 0.0009661588 3.503096e-01 4.945018e-01 1 961325 961388 64 + 1.481 1.428 -0.184
ENSG00000187961 E017 39.866358 0.0005098851 4.667752e-01 6.063172e-01 1 961389 961448 60 + 1.614 1.582 -0.110
ENSG00000187961 E018 62.913169 0.0003880719 1.500722e-01 2.617762e-01 1 961449 961552 104 + 1.820 1.765 -0.188
ENSG00000187961 E019 23.452720 0.0019905181 2.507411e-06 1.909404e-05 1 961553 961628 76 + 1.540 1.203 -1.169
ENSG00000187961 E020 98.546632 0.0011182071 9.305113e-01 9.603017e-01 1 961629 961750 122 + 1.980 1.986 0.020
ENSG00000187961 E021 27.531559 0.0007419189 2.227298e-07 2.116335e-06 1 961751 961825 75 + 1.614 1.282 -1.145
ENSG00000187961 E022 101.821005 0.0047561308 8.337804e-02 1.648582e-01 1 961826 961980 155 + 1.940 2.035 0.318
ENSG00000187961 E023 61.941629 0.0120593073 3.279663e-03 1.109068e-02 1 961981 962047 67 + 1.644 1.863 0.741
ENSG00000187961 E024 50.164314 0.0072189217 3.833704e-12 8.543508e-11 1 962048 962285 238 + 1.900 1.464 -1.482
ENSG00000187961 E025 53.928669 0.0005065470 2.312855e-05 1.413066e-04 1 962286 962313 28 + 1.827 1.633 -0.659
ENSG00000187961 E026 57.487376 0.0078858825 4.197101e-03 1.369612e-02 1 962314 962354 41 + 1.839 1.678 -0.545
ENSG00000187961 E027 128.455652 0.0002627289 5.681601e-01 6.948532e-01 1 962355 962471 117 + 2.079 2.106 0.090
ENSG00000187961 E028 15.702107 0.0174475182 4.082024e-03 1.337289e-02 1 962472 962478 7 + 1.349 1.067 -0.999
ENSG00000187961 E029 59.692413 0.0004619087 1.521014e-01 2.645455e-01 1 962704 962726 23 + 1.716 1.792 0.258
ENSG00000187961 E030 146.863836 0.0002616722 2.502807e-05 1.517326e-04 1 962727 962913 187 + 2.063 2.200 0.458
ENSG00000187961 E031 58.493153 0.0004727455 5.249775e-02 1.136075e-01 1 962914 962917 4 + 1.691 1.792 0.342
ENSG00000187961 E032 41.785909 0.0092806604 2.633844e-02 6.462555e-02 1 963032 963108 77 + 1.697 1.552 -0.496
ENSG00000187961 E033 156.863518 0.0029955786 6.121940e-01 7.311846e-01 1 963109 963253 145 + 2.168 2.197 0.099
ENSG00000187961 E034 184.061501 0.0002790753 5.314039e-01 6.638633e-01 1 963337 963504 168 + 2.236 2.261 0.085
ENSG00000187961 E035 51.664490 0.0005881982 2.052807e-09 2.867342e-08 1 963505 963551 47 + 1.855 1.569 -0.969
ENSG00000187961 E036 128.150777 0.0018118325 1.372247e-09 1.982674e-08 1 963552 963919 368 + 2.206 1.998 -0.695
ENSG00000187961 E037 146.114359 0.0002555194 1.352026e-03 5.149731e-03 1 963920 964008 89 + 2.083 2.189 0.353
ENSG00000187961 E038 119.855397 0.0003045533 2.235925e-02 5.642741e-02 1 964107 964167 61 + 2.011 2.096 0.284
ENSG00000187961 E039 56.055203 0.0004643841 8.181226e-04 3.324629e-03 1 964168 964180 13 + 1.622 1.796 0.591
ENSG00000187961 E040 178.462284 0.0002730088 1.257470e-02 3.490971e-02 1 964349 964530 182 + 2.189 2.266 0.257
ENSG00000187961 E041 371.758943 0.0046502849 2.055836e-07 1.966037e-06 1 964963 966394 1432 + 2.434 2.628 0.647