ENSG00000187954

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000403000 ENSG00000187954 HEK293_OSMI2_2hA HEK293_TMG_2hB CYHR1 protein_coding protein_coding 50.34137 79.63576 29.53252 2.885707 1.533528 -1.430805 5.974145 5.408788 3.6864150 0.5519907 0.57277011 -0.5518436 0.13219167 0.06766667 0.12603333 0.05836667 7.776394e-02 2.572237e-50 FALSE TRUE
ENST00000424149 ENSG00000187954 HEK293_OSMI2_2hA HEK293_TMG_2hB CYHR1 protein_coding protein_coding 50.34137 79.63576 29.53252 2.885707 1.533528 -1.430805 7.782709 12.634565 4.1082171 0.7149688 1.33595397 -1.6184257 0.15533333 0.15893333 0.13613333 -0.02280000 7.694083e-01 2.572237e-50 FALSE TRUE
ENST00000438911 ENSG00000187954 HEK293_OSMI2_2hA HEK293_TMG_2hB CYHR1 protein_coding protein_coding 50.34137 79.63576 29.53252 2.885707 1.533528 -1.430805 2.869006 9.631121 0.0000000 0.3327916 0.00000000 -9.9130571 0.04405833 0.12116667 0.00000000 -0.12116667 2.572237e-50 2.572237e-50 FALSE TRUE
ENST00000524623 ENSG00000187954 HEK293_OSMI2_2hA HEK293_TMG_2hB CYHR1 protein_coding retained_intron 50.34137 79.63576 29.53252 2.885707 1.533528 -1.430805 2.398297 2.587516 1.9415571 0.3668553 0.11634487 -0.4125070 0.05123333 0.03223333 0.06573333 0.03350000 7.961201e-04 2.572237e-50   FALSE
ENST00000528558 ENSG00000187954 HEK293_OSMI2_2hA HEK293_TMG_2hB CYHR1 protein_coding retained_intron 50.34137 79.63576 29.53252 2.885707 1.533528 -1.430805 2.836949 6.005877 0.8403253 0.3528352 0.09341361 -2.8226884 0.05327917 0.07540000 0.02833333 -0.04706667 4.546932e-08 2.572237e-50 FALSE TRUE
ENST00000530374 ENSG00000187954 HEK293_OSMI2_2hA HEK293_TMG_2hB CYHR1 protein_coding protein_coding 50.34137 79.63576 29.53252 2.885707 1.533528 -1.430805 3.258193 1.690552 4.2829446 0.3069935 0.12693486 1.3359641 0.07359167 0.02116667 0.14563333 0.12446667 1.128823e-17 2.572237e-50 FALSE TRUE
ENST00000533173 ENSG00000187954 HEK293_OSMI2_2hA HEK293_TMG_2hB CYHR1 protein_coding retained_intron 50.34137 79.63576 29.53252 2.885707 1.533528 -1.430805 7.094303 8.997472 4.5534854 0.7142186 0.36318774 -0.9809861 0.14610417 0.11273333 0.15370000 0.04096667 2.195972e-02 2.572237e-50 FALSE TRUE
MSTRG.32265.4 ENSG00000187954 HEK293_OSMI2_2hA HEK293_TMG_2hB CYHR1 protein_coding   50.34137 79.63576 29.53252 2.885707 1.533528 -1.430805 9.765516 15.861664 6.4597947 0.9470854 0.64092283 -1.2946616 0.19667083 0.19920000 0.21763333 0.01843333 6.995456e-01 2.572237e-50 FALSE TRUE
MSTRG.32265.7 ENSG00000187954 HEK293_OSMI2_2hA HEK293_TMG_2hB CYHR1 protein_coding   50.34137 79.63576 29.53252 2.885707 1.533528 -1.430805 4.252387 9.710425 1.4191292 0.2334647 0.30235425 -2.7658830 0.06979583 0.12206667 0.04856667 -0.07350000 3.413922e-03 2.572237e-50 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000187954 E001 217.5125637 0.0025455964 2.884034e-02 6.968580e-02 8 144449582 144450333 752 - 2.193 2.268 0.250
ENSG00000187954 E002 223.2095209 0.0002195137 1.694103e-01 2.874235e-01 8 144450334 144450575 242 - 2.239 2.269 0.101
ENSG00000187954 E003 216.1358641 0.0062143949 3.799712e-01 5.242377e-01 8 144450576 144450770 195 - 2.226 2.257 0.104
ENSG00000187954 E004 115.1438217 0.0010117777 5.354646e-06 3.785296e-05 8 144451120 144451288 169 - 1.814 2.010 0.658
ENSG00000187954 E005 520.4234207 0.0008235202 7.584066e-16 2.911405e-14 8 144451289 144451811 523 - 2.481 2.661 0.600
ENSG00000187954 E006 227.4837455 0.0006578091 1.166060e-05 7.630623e-05 8 144451812 144451927 116 - 2.166 2.295 0.431
ENSG00000187954 E007 164.7038832 0.0002228675 4.308359e-07 3.860771e-06 8 144451928 144451993 66 - 1.992 2.161 0.566
ENSG00000187954 E008 140.1272012 0.0002416089 3.851763e-03 1.272615e-02 8 144451994 144452035 42 - 1.982 2.080 0.328
ENSG00000187954 E009 247.0057716 0.0001703407 9.741667e-08 9.912610e-07 8 144452036 144452216 181 - 2.189 2.331 0.475
ENSG00000187954 E010 121.3486678 0.0010000609 9.177167e-05 4.833032e-04 8 144452217 144452256 40 - 1.864 2.025 0.539
ENSG00000187954 E011 163.2209609 0.0002288049 1.669632e-08 1.974696e-07 8 144452257 144452358 102 - 1.969 2.162 0.646
ENSG00000187954 E012 468.3401761 0.0022876171 6.105145e-02 1.284028e-01 8 144452359 144452570 212 - 2.547 2.594 0.155
ENSG00000187954 E013 423.7671604 0.0002063338 4.378637e-01 5.797438e-01 8 144453222 144453460 239 - 2.557 2.533 -0.081
ENSG00000187954 E014 535.2783292 0.0008338297 2.879647e-52 1.976596e-49 8 144453461 144457928 4468 - 2.860 2.554 -1.022
ENSG00000187954 E015 109.4436062 0.0034161481 6.674176e-10 1.023228e-08 8 144462283 144462833 551 - 2.163 1.877 -0.957
ENSG00000187954 E016 13.7747018 0.0012844664 2.926140e-14 8.984577e-13 8 144462834 144462871 38 - 1.516 0.831 -2.462
ENSG00000187954 E017 2.9968255 0.0248568238 8.939523e-05 4.721267e-04 8 144463817 144463824 8 - 0.924 0.287 -2.977
ENSG00000187954 E018 244.1065640 0.0014512156 3.143112e-01 4.570284e-01 8 144463825 144464274 450 - 2.333 2.295 -0.128
ENSG00000187954 E019 174.6092059 0.0002560314 7.239176e-01 8.176278e-01 8 144464275 144464459 185 - 2.152 2.155 0.008
ENSG00000187954 E020 165.2781259 0.0002184503 6.520547e-01 7.627886e-01 8 144464460 144464577 118 - 2.125 2.131 0.020
ENSG00000187954 E021 75.2988851 0.0006192551 9.967235e-01 1.000000e+00 8 144464578 144464580 3 - 1.799 1.791 -0.027
ENSG00000187954 E022 94.9363551 0.0007572439 9.736306e-01 9.875435e-01 8 144464581 144464612 32 - 1.897 1.890 -0.022
ENSG00000187954 E023 36.2864434 0.0042026965 3.872009e-06 2.826310e-05 8 144464613 144464808 196 - 1.708 1.388 -1.094
ENSG00000187954 E024 229.5008352 0.0002484771 7.190464e-02 1.465026e-01 8 144464809 144465096 288 - 2.318 2.260 -0.192
ENSG00000187954 E025 43.2417300 0.0039364294 3.520534e-04 1.588340e-03 8 144465097 144465207 111 - 1.731 1.495 -0.805
ENSG00000187954 E026 41.6706180 0.0112136874 1.079719e-03 4.234192e-03 8 144465208 144465352 145 - 1.727 1.470 -0.878
ENSG00000187954 E027 35.3447945 0.0131258199 8.511651e-03 2.506656e-02 8 144465353 144465409 57 - 1.634 1.411 -0.765
ENSG00000187954 E028 30.7491250 0.0140351724 9.050355e-02 1.759266e-01 8 144465410 144465570 161 - 1.531 1.373 -0.546
ENSG00000187954 E029 16.1798235 0.0391793868 7.415399e-01 8.306808e-01 8 144465571 144465667 97 - 1.112 1.158 0.163
ENSG00000187954 E030 0.9661334 0.1537080597 7.107076e-01 8.078178e-01 8 144466796 144466952 157 - 0.196 0.282 0.682