ENSG00000187325

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000341864 ENSG00000187325 HEK293_OSMI2_2hA HEK293_TMG_2hB TAF9B protein_coding protein_coding 21.93583 10.5993 37.44918 1.800617 0.08508935 1.81999 19.779994 10.27072 32.910856 1.726755 1.113272 1.67906 0.91945000 0.9696 0.87883333 -0.09076667 0.0045441339 0.0007260899 FALSE  
MSTRG.34523.3 ENSG00000187325 HEK293_OSMI2_2hA HEK293_TMG_2hB TAF9B protein_coding   21.93583 10.5993 37.44918 1.800617 0.08508935 1.81999 1.175171 0.00000 2.398732 0.000000 1.048481 7.91213 0.03650417 0.0000 0.06403333 0.06403333 0.0007260899 0.0007260899 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000187325 E001 7.730310 0.0021493435 3.636547e-02 8.427830e-02 X 78129385 78129727 343 - 0.757 1.131 1.408
ENSG00000187325 E002 991.357772 0.0085125736 1.664732e-11 3.344063e-10 X 78129748 78131613 1866 - 2.824 3.130 1.018
ENSG00000187325 E003 171.868557 0.0002755962 7.410367e-06 5.071241e-05 X 78131614 78131720 107 - 2.150 2.144 -0.019
ENSG00000187325 E004 120.326906 0.0008753202 1.540474e-03 5.767440e-03 X 78131721 78131773 53 - 1.994 2.005 0.038
ENSG00000187325 E005 184.111268 0.0004720976 7.135920e-07 6.117375e-06 X 78133338 78133448 111 - 2.184 2.163 -0.068
ENSG00000187325 E006 131.811725 0.0002957350 7.471988e-08 7.777297e-07 X 78136915 78136990 76 - 2.049 1.984 -0.219
ENSG00000187325 E007 171.057457 0.0063178570 5.051695e-03 1.607317e-02 X 78137749 78137883 135 - 2.148 2.137 -0.037
ENSG00000187325 E008 1.488524 0.0155116423 2.880481e-01 4.285905e-01 X 78137884 78137961 78 - 0.375 0.229 -0.978
ENSG00000187325 E009 186.267894 0.0022644494 1.244393e-06 1.012918e-05 X 78137962 78138098 137 - 2.193 2.142 -0.169
ENSG00000187325 E010 137.945487 0.0002992530 2.285985e-11 4.491993e-10 X 78138843 78138924 82 - 2.076 1.961 -0.386
ENSG00000187325 E011 108.352096 0.0004012346 4.099765e-08 4.500193e-07 X 78139561 78139692 132 - 1.969 1.874 -0.322