ENSG00000186665

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000334461 ENSG00000186665 HEK293_OSMI2_2hA HEK293_TMG_2hB C17orf58 protein_coding protein_coding 9.074002 7.8577 11.42041 0.8823466 0.2868169 0.5388626 0.2461846 0.5934425 0.000000 0.3010080 0.0000000 -5.9151445 0.04502917 0.06933333 0.0000000 -0.06933333 8.664465e-02 1.010878e-05 FALSE FALSE
ENST00000449250 ENSG00000186665 HEK293_OSMI2_2hA HEK293_TMG_2hB C17orf58 protein_coding protein_coding 9.074002 7.8577 11.42041 0.8823466 0.2868169 0.5388626 3.5786992 2.2077362 5.792845 0.3502118 0.1667231 1.3876726 0.35111667 0.27816667 0.5080667 0.22990000 1.010878e-05 1.010878e-05 FALSE FALSE
ENST00000536693 ENSG00000186665 HEK293_OSMI2_2hA HEK293_TMG_2hB C17orf58 protein_coding retained_intron 9.074002 7.8577 11.42041 0.8823466 0.2868169 0.5388626 3.5560027 2.5600910 4.365067 0.2554626 0.1963413 0.7674858 0.39481250 0.34133333 0.3820333 0.04070000 8.133807e-01 1.010878e-05 TRUE FALSE
ENST00000580729 ENSG00000186665 HEK293_OSMI2_2hA HEK293_TMG_2hB C17orf58 protein_coding protein_coding 9.074002 7.8577 11.42041 0.8823466 0.2868169 0.5388626 1.6931156 2.4964306 1.262493 0.5742646 0.3353253 -0.9779767 0.20902917 0.31116667 0.1098333 -0.20133333 8.364384e-03 1.010878e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000186665 E001 2.064665 0.0677384995 0.0021539949 0.0077017188 17 67991099 67991101 3 - 0.114 0.712 3.793
ENSG00000186665 E002 6.158904 0.0083669852 0.0668170361 0.1380887333 17 67991102 67991129 28 - 0.740 0.975 0.911
ENSG00000186665 E003 64.380076 0.0015895173 0.0001034369 0.0005379324 17 67991130 67991335 206 - 1.725 1.898 0.582
ENSG00000186665 E004 319.428930 0.0003937878 0.0029252206 0.0100452121 17 67991336 67992103 768 - 2.488 2.531 0.144
ENSG00000186665 E005 30.103904 0.0009842661 0.5809147343 0.7055734591 17 67992901 67993043 143 - 1.507 1.472 -0.119
ENSG00000186665 E006 113.815255 0.0004177467 0.0007676099 0.0031420123 17 67993044 67993235 192 - 2.112 2.009 -0.345
ENSG00000186665 E007 35.825148 0.0116589437 0.0022980247 0.0081443060 17 67993236 67993423 188 - 1.671 1.429 -0.825
ENSG00000186665 E008 49.700159 0.0006270745 0.0000670601 0.0003659188 17 67993424 67993984 561 - 1.795 1.601 -0.658
ENSG00000186665 E009 7.825049 0.0022678458 0.0062526714 0.0192764352 17 67996123 67996469 347 - 0.763 1.078 1.194