ENSG00000186532

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000305513 ENSG00000186532 HEK293_OSMI2_2hA HEK293_TMG_2hB SMYD4 protein_coding protein_coding 11.42658 12.44758 10.39805 0.6918038 0.2989963 -0.2593244 6.9452137 7.4844978 5.6466156 0.15164169 0.33007394 -0.4058927 0.60903333 0.60383333 0.54260000 -0.06123333 4.738929e-01 4.866204e-05 FALSE TRUE
ENST00000491788 ENSG00000186532 HEK293_OSMI2_2hA HEK293_TMG_2hB SMYD4 protein_coding protein_coding 11.42658 12.44758 10.39805 0.6918038 0.2989963 -0.2593244 1.3081819 0.8018679 1.3044571 0.07082488 0.12720744 0.6951502 0.11602917 0.06416667 0.12533333 0.06116667 7.090878e-03 4.866204e-05 FALSE TRUE
MSTRG.13380.1 ENSG00000186532 HEK293_OSMI2_2hA HEK293_TMG_2hB SMYD4 protein_coding   11.42658 12.44758 10.39805 0.6918038 0.2989963 -0.2593244 0.2907561 0.0000000 0.9808571 0.00000000 0.55013468 6.6306051 0.02757500 0.00000000 0.09680000 0.09680000 1.776140e-01 4.866204e-05 TRUE TRUE
MSTRG.13380.12 ENSG00000186532 HEK293_OSMI2_2hA HEK293_TMG_2hB SMYD4 protein_coding   11.42658 12.44758 10.39805 0.6918038 0.2989963 -0.2593244 1.5444953 3.2890505 1.1731436 0.45001408 0.07818508 -1.4794257 0.13116250 0.26186667 0.11316667 -0.14870000 4.866204e-05 4.866204e-05   FALSE
MSTRG.13380.2 ENSG00000186532 HEK293_OSMI2_2hA HEK293_TMG_2hB SMYD4 protein_coding   11.42658 12.44758 10.39805 0.6918038 0.2989963 -0.2593244 0.6964009 0.4240591 0.6071573 0.11242615 0.37537218 0.5077467 0.06076667 0.03340000 0.05683333 0.02343333 1.000000e+00 4.866204e-05 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000186532 E001 0.5889961 0.8532422427 2.593587e-01 3.967341e-01 17 1744063 1744125 63 - 0.378 0.000 -10.887
ENSG00000186532 E002 0.0000000       17 1761723 1761765 43 -      
ENSG00000186532 E003 353.5429730 0.0045759389 6.833673e-09 8.689884e-08 17 1779485 1781151 1667 - 2.411 2.620 0.698
ENSG00000186532 E004 104.2894503 0.0003014546 4.964681e-02 1.085364e-01 17 1781152 1781439 288 - 1.957 2.050 0.313
ENSG00000186532 E005 45.5128736 0.0007039806 1.382117e-03 5.247990e-03 17 1781692 1783034 1343 - 1.735 1.594 -0.477
ENSG00000186532 E006 53.6085236 0.0004524505 1.211080e-01 2.215206e-01 17 1783035 1783109 75 - 1.669 1.769 0.339
ENSG00000186532 E007 40.1827545 0.0093590569 1.220574e-01 2.228741e-01 17 1783110 1783158 49 - 1.524 1.662 0.471
ENSG00000186532 E008 60.9298938 0.0011862137 5.904209e-01 7.132829e-01 17 1783360 1783476 117 - 1.757 1.807 0.172
ENSG00000186532 E009 2.1327103 0.0070953776 9.402367e-01 9.664635e-01 17 1783477 1783863 387 - 0.490 0.495 0.025
ENSG00000186532 E010 61.9309568 0.0005289415 2.049686e-01 3.323705e-01 17 1784326 1784461 136 - 1.743 1.826 0.279
ENSG00000186532 E011 81.2739375 0.0003803240 7.691567e-01 8.508574e-01 17 1786810 1786973 164 - 1.884 1.921 0.124
ENSG00000186532 E012 96.7561977 0.0003044279 1.108030e-03 4.329886e-03 17 1787422 1787604 183 - 2.032 1.943 -0.300
ENSG00000186532 E013 219.0777386 0.0011112938 6.332763e-06 4.405563e-05 17 1799857 1800723 867 - 2.386 2.294 -0.307
ENSG00000186532 E014 46.4005264 0.0033265569 6.088411e-02 1.281234e-01 17 1800724 1800805 82 - 1.712 1.631 -0.276
ENSG00000186532 E015 44.3733433 0.0046628013 2.821224e-03 9.737688e-03 17 1800806 1800861 56 - 1.732 1.578 -0.524
ENSG00000186532 E016 58.9466490 0.0004321638 2.592588e-03 9.043101e-03 17 1800862 1801024 163 - 1.834 1.724 -0.372
ENSG00000186532 E017 0.0000000       17 1804346 1804625 280 -      
ENSG00000186532 E018 41.3585844 0.0005567083 4.050675e-04 1.794221e-03 17 1804626 1804715 90 - 1.706 1.541 -0.563
ENSG00000186532 E019 37.3719783 0.0006109031 9.679062e-04 3.848689e-03 17 1811971 1812061 91 - 1.662 1.501 -0.550
ENSG00000186532 E020 25.2197143 0.0007655249 5.171829e-03 1.639510e-02 17 1812062 1812115 54 - 1.501 1.336 -0.569
ENSG00000186532 E021 3.7246887 0.0041746045 1.355229e-01 2.418487e-01 17 1814566 1814795 230 - 0.769 0.572 -0.834
ENSG00000186532 E022 19.3187122 0.0009599666 5.050970e-06 3.590984e-05 17 1821253 1821507 255 - 1.017 1.429 1.457
ENSG00000186532 E023 68.3870876 0.0005470377 8.505129e-01 9.072523e-01 17 1827861 1828006 146 - 1.814 1.847 0.112
ENSG00000186532 E024 0.9996463 0.0123202311 8.135359e-01 8.817801e-01 17 1829349 1829725 377 - 0.311 0.281 -0.197
ENSG00000186532 E025 41.4414895 0.0031243988 4.574456e-01 5.977700e-01 17 1829726 1829902 177 - 1.628 1.609 -0.066
ENSG00000186532 E026 0.0000000       17 1830593 1830634 42 -