ENSG00000186523

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000340537 ENSG00000186523 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM86B1 protein_coding nonsense_mediated_decay 5.847962 7.073653 5.506749 0.5961636 0.3536858 -0.3606751 1.4578450 2.5626523 0.6223287 0.27337309 0.36233747 -2.0245100 0.25386250 0.36210000 0.12206667 -0.2400333333 3.338677e-01 1.994951e-05 TRUE FALSE
ENST00000524571 ENSG00000186523 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM86B1 protein_coding protein_coding 5.847962 7.073653 5.506749 0.5961636 0.3536858 -0.3606751 0.3218503 0.2893301 0.2356659 0.06307570 0.11862488 -0.2850396 0.05415417 0.04006667 0.04020000 0.0001333333 9.156988e-01 1.994951e-05 FALSE TRUE
ENST00000530508 ENSG00000186523 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM86B1 protein_coding retained_intron 5.847962 7.073653 5.506749 0.5961636 0.3536858 -0.3606751 0.4719866 0.4343449 0.2860206 0.04703683 0.07649136 -0.5859824 0.08399583 0.06233333 0.05156667 -0.0107666667 8.336805e-01 1.994951e-05   FALSE
ENST00000531833 ENSG00000186523 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM86B1 protein_coding nonsense_mediated_decay 5.847962 7.073653 5.506749 0.5961636 0.3536858 -0.3606751 0.4361098 0.0321048 0.4968841 0.03210480 0.10923923 3.5895992 0.07353750 0.00500000 0.08920000 0.0842000000 1.994951e-05 1.994951e-05 TRUE FALSE
ENST00000534187 ENSG00000186523 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM86B1 protein_coding nonsense_mediated_decay 5.847962 7.073653 5.506749 0.5961636 0.3536858 -0.3606751 0.3074974 0.7219173 0.1859398 0.03050676 0.12252643 -1.9012706 0.04690000 0.10303333 0.03700000 -0.0660333333 2.633496e-01 1.994951e-05 FALSE FALSE
MSTRG.31171.1 ENSG00000186523 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM86B1 protein_coding   5.847962 7.073653 5.506749 0.5961636 0.3536858 -0.3606751 1.3529057 1.7077318 1.5163591 0.04046590 0.11422012 -0.1704104 0.22677500 0.24513333 0.27523333 0.0301000000 7.353269e-01 1.994951e-05 FALSE FALSE
MSTRG.31171.12 ENSG00000186523 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM86B1 protein_coding   5.847962 7.073653 5.506749 0.5961636 0.3536858 -0.3606751 0.3699980 0.0950329 0.8396138 0.09503290 0.42551541 3.0159659 0.05944167 0.01150000 0.14330000 0.1318000000 4.140377e-01 1.994951e-05 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000186523 E001 0.0000000       8 12181956 12182095 140 -      
ENSG00000186523 E002 0.0000000       8 12182096 12182097 2 -      
ENSG00000186523 E003 0.0000000       8 12182098 12182105 8 -      
ENSG00000186523 E004 0.0000000       8 12182106 12182109 4 -      
ENSG00000186523 E005 34.0929682 0.008175412 0.740234021 0.82970030 8 12182110 12182555 446 - 1.515 1.481 -0.119
ENSG00000186523 E006 37.2360135 0.001074089 0.122402915 0.22337365 8 12182556 12183120 565 - 1.475 1.548 0.250
ENSG00000186523 E007 21.2324373 0.019430618 0.540755664 0.67185489 8 12183121 12183392 272 - 1.355 1.276 -0.277
ENSG00000186523 E008 5.8016843 0.121748759 0.830326072 0.89353685 8 12183393 12183440 48 - 0.798 0.794 -0.018
ENSG00000186523 E009 2.9734794 0.044526963 0.613607022 0.73226122 8 12183441 12183452 12 - 0.640 0.534 -0.478
ENSG00000186523 E010 3.3388510 0.425467701 0.716702917 0.81227360 8 12183453 12183479 27 - 0.637 0.595 -0.188
ENSG00000186523 E011 14.3597639 0.001394016 0.295516807 0.43665101 8 12183480 12183706 227 - 1.082 1.165 0.300
ENSG00000186523 E012 0.0000000       8 12185149 12185233 85 -      
ENSG00000186523 E013 0.0000000       8 12185234 12185375 142 -      
ENSG00000186523 E014 12.8223038 0.001703013 0.165141162 0.28187693 8 12185376 12185454 79 - 1.006 1.131 0.454
ENSG00000186523 E015 3.5748556 0.134573578 0.504238696 0.64013796 8 12185455 12185495 41 - 0.532 0.666 0.593
ENSG00000186523 E016 1.2543018 0.024558594 0.744114449 0.83256212 8 12185496 12185525 30 - 0.388 0.321 -0.398
ENSG00000186523 E017 0.0000000       8 12185526 12185547 22 -      
ENSG00000186523 E018 0.0000000       8 12185548 12186061 514 -      
ENSG00000186523 E019 0.2998086 0.029323180 0.582795626   8 12186062 12186351 290 - 0.171 0.086 -1.133
ENSG00000186523 E020 2.0463211 0.100652286 0.169172623 0.28710244 8 12186352 12186453 102 - 0.641 0.362 -1.374
ENSG00000186523 E021 3.1179761 0.033599037 0.005347261 0.01687668 8 12186454 12186617 164 - 0.861 0.404 -2.031
ENSG00000186523 E022 0.5911862 0.019387492 0.005160239 0.01636493 8 12186618 12186699 82 - 0.466 0.000 -13.603
ENSG00000186523 E023 1.1500158 0.012251599 0.553569611 0.68267664 8 12186700 12186833 134 - 0.388 0.274 -0.716
ENSG00000186523 E024 0.1515154 0.043720517 0.232573909   8 12188072 12188173 102 - 0.171 0.000 -11.717
ENSG00000186523 E025 0.1515154 0.043720517 0.232573909   8 12188174 12188216 43 - 0.171 0.000 -11.717
ENSG00000186523 E026 0.2998086 0.029323180 0.582795626   8 12188311 12188424 114 - 0.171 0.086 -1.133
ENSG00000186523 E027 0.5169874 0.020433454 0.980434212 0.99176603 8 12189808 12189888 81 - 0.171 0.158 -0.129
ENSG00000186523 E028 0.6986918 0.017025885 0.764242937 0.84736620 8 12191779 12191841 63 - 0.171 0.220 0.456
ENSG00000186523 E029 0.0000000       8 12191842 12192012 171 -      
ENSG00000186523 E030 2.0605878 0.007243862 0.592278623 0.71484378 8 12193580 12193610 31 - 0.388 0.474 0.455
ENSG00000186523 E031 2.9441932 0.006889726 0.811586587 0.88042789 8 12193611 12193684 74 - 0.532 0.561 0.134
ENSG00000186523 E032 0.2965864 0.153935431 0.541591365   8 12193877 12193974 98 - 0.000 0.156 10.024
ENSG00000186523 E033 0.5149111 0.020216071 0.990884376 0.99844108 8 12193975 12194004 30 - 0.171 0.158 -0.129
ENSG00000186523 E034 0.6632043 0.017858120 0.766426084 0.84892010 8 12194005 12194133 129 - 0.171 0.220 0.454