ENSG00000186185

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000585687 ENSG00000186185 HEK293_OSMI2_2hA HEK293_TMG_2hB KIF18B protein_coding retained_intron 40.18094 42.56968 34.50878 2.41613 0.955923 -0.3027836 6.438746 2.366709 7.108420 0.1780242 0.6001634 1.5825919 0.16523750 0.0557 0.20536667 0.14966667 2.405400e-19 2.4054e-19 FALSE FALSE
ENST00000587309 ENSG00000186185 HEK293_OSMI2_2hA HEK293_TMG_2hB KIF18B protein_coding protein_coding 40.18094 42.56968 34.50878 2.41613 0.955923 -0.3027836 30.067037 35.651772 24.863227 2.5422409 0.3412045 -0.5197847 0.74322500 0.8360 0.72103333 -0.11496667 1.507772e-04 2.4054e-19 FALSE TRUE
ENST00000593135 ENSG00000186185 HEK293_OSMI2_2hA HEK293_TMG_2hB KIF18B protein_coding protein_coding 40.18094 42.56968 34.50878 2.41613 0.955923 -0.3027836 3.235043 4.354383 2.067082 0.2830682 0.1068330 -1.0712196 0.08026667 0.1037 0.06003333 -0.04366667 3.551201e-02 2.4054e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000186185 E001 0.2214452 0.0380122204 7.508755e-01   17 44924570 44924708 139 - 0.000 0.102 9.830
ENSG00000186185 E002 0.4031496 0.0245398330 3.628817e-01 5.072894e-01 17 44924709 44924710 2 - 0.000 0.184 10.831
ENSG00000186185 E003 456.3472938 0.0038330775 2.671769e-14 8.249001e-13 17 44924711 44925005 295 - 2.427 2.723 0.987
ENSG00000186185 E004 778.5928686 0.0044582945 1.556484e-10 2.657695e-09 17 44925006 44925420 415 - 2.698 2.943 0.814
ENSG00000186185 E005 550.2178428 0.0012023506 8.770216e-13 2.174442e-11 17 44925421 44925753 333 - 2.586 2.778 0.640
ENSG00000186185 E006 651.2643323 0.0023320587 3.519863e-02 8.202162e-02 17 44925754 44926061 308 - 2.736 2.822 0.287
ENSG00000186185 E007 370.1247070 0.0035254684 5.734951e-01 6.994227e-01 17 44926062 44926186 125 - 2.517 2.565 0.163
ENSG00000186185 E008 354.9436959 0.0004741987 7.399362e-01 8.295015e-01 17 44926187 44926359 173 - 2.512 2.533 0.070
ENSG00000186185 E009 193.7667326 0.0047693226 3.976541e-01 5.414013e-01 17 44926360 44926413 54 - 2.268 2.264 -0.011
ENSG00000186185 E010 279.2107957 0.0026985045 7.407840e-01 8.301243e-01 17 44926414 44926499 86 - 2.398 2.439 0.137
ENSG00000186185 E011 239.3874933 0.0043128752 4.772452e-01 6.158323e-01 17 44926989 44927078 90 - 2.320 2.378 0.195
ENSG00000186185 E012 686.0848325 0.0009960215 4.643469e-01 6.040840e-01 17 44928026 44928578 553 - 2.803 2.819 0.054
ENSG00000186185 E013 370.9371458 0.0005051417 3.930327e-07 3.548195e-06 17 44928819 44929024 206 - 2.597 2.519 -0.262
ENSG00000186185 E014 286.6933557 0.0001908280 7.878040e-05 4.222222e-04 17 44931602 44931729 128 - 2.473 2.414 -0.195
ENSG00000186185 E015 232.9211707 0.0001984862 1.026211e-01 1.945001e-01 17 44932056 44932206 151 - 2.353 2.341 -0.041
ENSG00000186185 E016 107.4811835 0.0002822051 4.739983e-01 6.128689e-01 17 44932207 44932242 36 - 2.013 2.014 0.006
ENSG00000186185 E017 58.6966606 0.0005518427 4.728198e-32 9.158926e-30 17 44932322 44932672 351 - 2.019 1.496 -1.772
ENSG00000186185 E018 190.3508617 0.0001962004 6.642189e-03 2.029520e-02 17 44932673 44932773 101 - 2.287 2.242 -0.150
ENSG00000186185 E019 167.1707364 0.0022004761 5.502931e-02 1.180476e-01 17 44932912 44932986 75 - 2.227 2.188 -0.132
ENSG00000186185 E020 284.1825641 0.0003227659 3.039015e-09 4.118256e-08 17 44933923 44934099 177 - 2.496 2.390 -0.352
ENSG00000186185 E021 267.4920649 0.0003234072 1.349104e-12 3.236466e-11 17 44934233 44934430 198 - 2.486 2.351 -0.452
ENSG00000186185 E022 172.0523282 0.0003148076 3.970503e-06 2.890742e-05 17 44934507 44934617 111 - 2.277 2.174 -0.343
ENSG00000186185 E023 123.2205826 0.0087978737 2.359742e-02 5.898251e-02 17 44934831 44934935 105 - 2.133 2.038 -0.319
ENSG00000186185 E024 190.5735848 0.0116140950 5.671698e-03 1.774427e-02 17 44935259 44935416 158 - 2.338 2.213 -0.417
ENSG00000186185 E025 311.8988225 0.0071920683 6.325608e-05 3.473630e-04 17 44936032 44936358 327 - 2.563 2.417 -0.489
ENSG00000186185 E026 2.6635014 0.1410648840 8.494558e-01 9.065893e-01 17 44936359 44936371 13 - 0.533 0.536 0.017
ENSG00000186185 E027 3.9597617 0.0048663298 4.512482e-01 5.922286e-01 17 44947221 44947344 124 - 0.734 0.646 -0.365
ENSG00000186185 E028 113.5011915 0.0036784711 2.560713e-05 1.548616e-04 17 44947628 44947773 146 - 2.131 1.973 -0.528