ENSG00000186184

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000302979 ENSG00000186184 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR1D protein_coding protein_coding 151.4816 197.9663 145.8525 19.33552 2.003481 -0.4407187 97.333370 137.480461 82.29574 17.632726 1.6399046 -0.7402666 0.63350833 0.69046667 0.56433333 -0.1261333 1.976218e-03 5.658137e-07 FALSE TRUE
ENST00000399697 ENSG00000186184 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR1D protein_coding protein_coding 151.4816 197.9663 145.8525 19.33552 2.003481 -0.4407187 25.668547 33.276101 23.65861 1.588319 0.9728771 -0.4919450 0.17154167 0.16970000 0.16230000 -0.0074000 8.763036e-01 5.658137e-07 FALSE FALSE
ENST00000636117 ENSG00000186184 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR1D protein_coding processed_transcript 151.4816 197.9663 145.8525 19.33552 2.003481 -0.4407187 5.222426 2.793246 10.49193 1.400122 3.4863759 1.9054836 0.03539167 0.01556667 0.07176667 0.0562000 4.884144e-01 5.658137e-07   FALSE
MSTRG.8526.6 ENSG00000186184 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR1D protein_coding   151.4816 197.9663 145.8525 19.33552 2.003481 -0.4407187 14.456819 15.883166 19.06257 1.575267 0.6485676 0.2630928 0.09784167 0.08033333 0.13073333 0.0504000 5.757596e-06 5.658137e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000186184 E001 0.1482932 4.064565e-02 6.526625e-01   13 27620742 27620742 1 + 0.000 0.109 9.107
ENSG00000186184 E002 2.3710081 6.829492e-03 3.607944e-01 5.051543e-01 13 27620743 27620757 15 + 0.599 0.476 -0.576
ENSG00000186184 E003 4.1769536 5.119465e-03 8.062866e-01 8.768630e-01 13 27620758 27620764 7 + 0.706 0.698 -0.034
ENSG00000186184 E004 7.9641826 8.614612e-03 7.063034e-01 8.044652e-01 13 27620765 27620771 7 + 0.945 0.926 -0.070
ENSG00000186184 E005 51.0071146 4.681301e-04 6.116613e-06 4.269774e-05 13 27620772 27620918 147 + 1.812 1.624 -0.638
ENSG00000186184 E006 56.7015231 1.983552e-03 6.150893e-05 3.389280e-04 13 27620919 27620946 28 + 1.852 1.685 -0.565
ENSG00000186184 E007 57.6080573 2.002059e-03 7.754843e-05 4.164551e-04 13 27620947 27620962 16 + 1.857 1.693 -0.554
ENSG00000186184 E008 76.1421101 8.287507e-04 2.902264e-07 2.694297e-06 13 27620963 27620994 32 + 1.983 1.802 -0.608
ENSG00000186184 E009 144.1814943 2.583073e-04 2.943503e-07 2.728708e-06 13 27620995 27621104 110 + 2.223 2.104 -0.399
ENSG00000186184 E010 83.9716347 3.213858e-04 2.717155e-05 1.633164e-04 13 27621105 27621140 36 + 1.994 1.866 -0.432
ENSG00000186184 E011 14.0551526 2.162146e-02 1.902573e-02 4.939182e-02 13 27621141 27621192 52 + 1.292 1.054 -0.850
ENSG00000186184 E012 16.2429593 1.188689e-03 8.028692e-04 3.269516e-03 13 27621193 27621263 71 + 1.360 1.113 -0.872
ENSG00000186184 E013 18.7238180 1.074575e-03 3.532379e-06 2.603481e-05 13 27621264 27621336 73 + 1.455 1.123 -1.165
ENSG00000186184 E014 160.5334543 1.676837e-03 1.785270e-07 1.727874e-06 13 27621337 27621437 101 + 2.284 2.138 -0.488
ENSG00000186184 E015 203.0013775 2.294562e-04 1.970379e-25 2.245983e-23 13 27621438 27621778 341 + 2.427 2.198 -0.765
ENSG00000186184 E016 70.9863676 3.613443e-04 4.699821e-09 6.162562e-08 13 27621779 27621869 91 + 1.966 1.755 -0.710
ENSG00000186184 E017 122.7786332 6.775477e-04 5.729782e-06 4.023761e-05 13 27621870 27621872 3 + 2.154 2.034 -0.402
ENSG00000186184 E018 128.3619703 9.100256e-04 1.070252e-05 7.065408e-05 13 27621873 27621874 2 + 2.172 2.055 -0.391
ENSG00000186184 E019 310.6462154 2.573593e-04 6.329931e-04 2.654770e-03 13 27621875 27621891 17 + 2.510 2.472 -0.129
ENSG00000186184 E020 329.8046483 5.204926e-04 1.448877e-02 3.933525e-02 13 27621892 27621892 1 + 2.526 2.505 -0.070
ENSG00000186184 E021 419.6682903 3.438852e-04 2.821963e-01 4.221688e-01 13 27621893 27621904 12 + 2.610 2.623 0.042
ENSG00000186184 E022 702.8558519 1.648673e-04 2.608007e-01 3.983893e-01 13 27621905 27621946 42 + 2.830 2.848 0.058
ENSG00000186184 E023 686.0366575 1.120732e-04 8.724615e-01 9.219755e-01 13 27621947 27621953 7 + 2.809 2.845 0.121
ENSG00000186184 E024 811.6699400 1.023472e-04 4.395695e-01 5.813151e-01 13 27621954 27621983 30 + 2.876 2.920 0.146
ENSG00000186184 E025 783.3281784 8.845787e-05 5.502034e-01 6.798530e-01 13 27621984 27622009 26 + 2.862 2.904 0.139
ENSG00000186184 E026 6.8662839 5.732763e-03 2.294299e-02 5.762653e-02 13 27622159 27622222 64 + 1.028 0.787 -0.915
ENSG00000186184 E027 4.0275927 4.025051e-03 2.600750e-02 6.396016e-02 13 27622354 27622390 37 + 0.842 0.552 -1.211
ENSG00000186184 E028 8.2589643 1.145586e-02 1.583571e-02 4.237088e-02 13 27622841 27622874 34 + 1.098 0.843 -0.948
ENSG00000186184 E029 1981.2328641 3.056731e-03 1.811217e-04 8.828077e-04 13 27622875 27623452 578 + 3.209 3.330 0.400
ENSG00000186184 E030 2.6131765 5.813506e-03 2.360472e-01 3.695953e-01 13 27646194 27646218 25 + 0.395 0.617 1.079
ENSG00000186184 E031 0.3268771 2.906830e-02 8.056865e-01   13 27648010 27648378 369 + 0.137 0.109 -0.381
ENSG00000186184 E032 215.9028921 1.666231e-03 1.941361e-02 5.023081e-02 13 27648379 27648386 8 + 2.352 2.309 -0.143
ENSG00000186184 E033 316.0897666 2.595790e-03 1.618953e-01 2.776019e-01 13 27648387 27648449 63 + 2.501 2.485 -0.053
ENSG00000186184 E034 164.5340694 4.290287e-03 2.658369e-01 4.039366e-01 13 27648450 27648453 4 + 2.161 2.238 0.257
ENSG00000186184 E035 1.9367246 7.258851e-02 1.276024e-01 2.308078e-01 13 27648454 27649606 1153 + 0.600 0.331 -1.382
ENSG00000186184 E036 16.9573988 5.564549e-02 3.576966e-01 5.021150e-01 13 27650042 27650241 200 + 1.301 1.193 -0.381
ENSG00000186184 E037 25.2632055 2.117957e-02 1.475200e-05 9.430162e-05 13 27650242 27651352 1111 + 1.607 1.202 -1.403
ENSG00000186184 E038 3.3203786 5.211988e-03 2.343905e-01 3.676211e-01 13 27651353 27651549 197 + 0.706 0.552 -0.670
ENSG00000186184 E039 0.6976476 2.024795e-02 7.396573e-01 8.293090e-01 13 27660779 27660811 33 + 0.241 0.196 -0.379
ENSG00000186184 E040 1.1813256 1.314108e-01 1.756782e-01 2.955721e-01 13 27660868 27660915 48 + 0.456 0.199 -1.678
ENSG00000186184 E041 3.1636795 8.503022e-03 1.662210e-01 2.832835e-01 13 27664627 27665112 486 + 0.706 0.517 -0.839
ENSG00000186184 E042 0.9201370 1.504956e-02 3.114033e-01 4.538521e-01 13 27665113 27665144 32 + 0.137 0.331 1.620
ENSG00000186184 E043 3.6503867 7.153696e-03 4.464482e-01 5.879329e-01 13 27665145 27665685 541 + 0.556 0.699 0.620
ENSG00000186184 E044 532.6351999 9.289524e-04 2.869700e-01 4.274261e-01 13 27665686 27665949 264 + 2.685 2.738 0.178
ENSG00000186184 E045 749.7185988 2.804835e-03 1.410078e-03 5.340030e-03 13 27665950 27666856 907 + 2.792 2.905 0.378
ENSG00000186184 E046 523.7393033 3.142338e-03 6.677194e-08 7.017888e-07 13 27666857 27667415 559 + 2.579 2.777 0.661
ENSG00000186184 E047 4.0846545 4.560106e-03 3.222675e-03 1.092332e-02 13 27738707 27738767 61 + 0.325 0.826 2.356
ENSG00000186184 E048 4.4888483 4.126442e-03 1.930900e-04 9.342438e-04 13 27739917 27744237 4321 + 0.241 0.879 3.143