ENSG00000185973

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000334398 ENSG00000185973 HEK293_OSMI2_2hA HEK293_TMG_2hB TMLHE protein_coding protein_coding 12.15913 14.49261 12.35581 2.474672 0.5240951 -0.2299556 3.0324306 2.9611720 3.5610947 0.6547367 0.1469539 0.2653342 0.24631250 0.2027333 0.28840000 0.085666667 0.04414117 0.04414117 FALSE  
ENST00000369439 ENSG00000185973 HEK293_OSMI2_2hA HEK293_TMG_2hB TMLHE protein_coding protein_coding 12.15913 14.49261 12.35581 2.474672 0.5240951 -0.2299556 6.5489157 6.7639178 7.4913550 1.5414870 0.6885388 0.1471607 0.53495833 0.4574667 0.60406667 0.146600000 0.07100968 0.04414117 FALSE  
ENST00000487422 ENSG00000185973 HEK293_OSMI2_2hA HEK293_TMG_2hB TMLHE protein_coding processed_transcript 12.15913 14.49261 12.35581 2.474672 0.5240951 -0.2299556 1.5830868 3.5825252 0.5861149 0.1182631 0.3891817 -2.5913359 0.13862083 0.2598000 0.04663333 -0.213166667 0.13878949 0.04414117 FALSE  
ENST00000675642 ENSG00000185973 HEK293_OSMI2_2hA HEK293_TMG_2hB TMLHE protein_coding protein_coding 12.15913 14.49261 12.35581 2.474672 0.5240951 -0.2299556 0.6586456 0.8872925 0.6027913 0.4696341 0.3824510 -0.5501826 0.05225833 0.0558000 0.05163333 -0.004166667 0.98768028 0.04414117 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185973 E001 2.5476175 0.0131538773 5.671971e-02 1.209820e-01 X 155489011 155490787 1777 - 0.700 0.390 -1.467
ENSG00000185973 E002 0.3289534 0.0329487976 8.800775e-01   X 155490788 155491448 661 - 0.135 0.111 -0.329
ENSG00000185973 E003 12.6294034 0.0120203009 1.084045e-01 2.031801e-01 X 155491449 155491666 218 - 1.021 1.196 0.632
ENSG00000185973 E004 1.1351654 0.0142605466 9.643154e-02 1.850010e-01 X 155492282 155492356 75 - 0.135 0.437 2.252
ENSG00000185973 E005 52.0687037 0.0004539205 3.946522e-02 9.003791e-02 X 155492357 155492495 139 - 1.665 1.768 0.350
ENSG00000185973 E006 3.4038516 0.0051374373 5.711352e-01 6.974830e-01 X 155492535 155492700 166 - 0.700 0.622 -0.333
ENSG00000185973 E007 132.5308615 0.0016889821 1.026300e-02 2.941382e-02 X 155506898 155507134 237 - 2.074 2.163 0.300
ENSG00000185973 E008 111.7226780 0.0051764229 2.112005e-01 3.399228e-01 X 155511673 155511792 120 - 2.082 2.034 -0.160
ENSG00000185973 E009 157.4499582 0.0003960299 4.877754e-04 2.113478e-03 X 155513986 155514265 280 - 2.251 2.164 -0.293
ENSG00000185973 E010 12.5847303 0.0329280098 5.404537e-07 4.749389e-06 X 155524271 155524455 185 - 0.593 1.295 2.681
ENSG00000185973 E011 136.2217303 0.0028370772 7.429314e-01 8.317296e-01 X 155524456 155524632 177 - 2.145 2.127 -0.061
ENSG00000185973 E012 121.9744853 0.0006777006 5.438824e-01 6.744134e-01 X 155545096 155545277 182 - 2.100 2.082 -0.059
ENSG00000185973 E013 1.8487222 0.0077480128 6.763873e-01 7.813768e-01 X 155612128 155612604 477 - 0.504 0.438 -0.332
ENSG00000185973 E014 68.9715743 0.0004253535 8.927547e-01 9.354674e-01 X 155612792 155612952 161 - 1.849 1.848 -0.003
ENSG00000185973 E015 0.0000000       X 155669758 155669941 184 -      
ENSG00000185973 E016 0.0000000       X 155719027 155719098 72 -