ENSG00000185947

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000300870 ENSG00000185947 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF267 protein_coding protein_coding 5.135658 1.693923 8.371331 0.06864261 0.123099 2.298319 4.6427551 1.6172006 7.061352 0.04812407 0.05247471 2.119594 0.92045417 0.95576667 0.8440667 -0.1117 0.01445650 0.0144565 FALSE TRUE
ENST00000394846 ENSG00000185947 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF267 protein_coding protein_coding 5.135658 1.693923 8.371331 0.06864261 0.123099 2.298319 0.4157063 0.0671145 1.074166 0.04154968 0.10615756 3.813440 0.06430417 0.03836667 0.1280667 0.0897 0.03054046 0.0144565 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185947 E001 15.7611348 0.0017933811 5.449225e-02 1.171090e-01 16 31873807 31873830 24 + 1.117 1.289 0.609
ENSG00000185947 E002 16.5886417 0.0012005954 6.307940e-02 1.318381e-01 16 31873831 31873832 2 + 1.140 1.302 0.572
ENSG00000185947 E003 46.2793797 0.0004661728 5.611762e-05 3.122672e-04 16 31873833 31873969 137 + 1.555 1.764 0.712
ENSG00000185947 E004 0.1472490 0.0434674010 1.000000e+00   16 31874066 31874264 199 + 0.071 0.000 -9.874
ENSG00000185947 E005 0.0000000       16 31875152 31875202 51 +      
ENSG00000185947 E006 1.0436561 0.0170934134 1.164724e-01 2.149194e-01 16 31875203 31875302 100 + 0.351 0.000 -12.866
ENSG00000185947 E007 65.7925683 0.0004412659 3.223670e-09 4.349200e-08 16 31884498 31884624 127 + 1.687 1.941 0.856
ENSG00000185947 E008 56.1841173 0.0004953331 3.222491e-07 2.962256e-06 16 31885161 31885256 96 + 1.628 1.867 0.808
ENSG00000185947 E009 4.1676729 0.0037865764 1.182445e-01 2.174121e-01 16 31890207 31890250 44 + 0.722 0.445 -1.257
ENSG00000185947 E010 0.4782907 0.0222208470 5.816965e-03 1.813107e-02 16 31911854 31914475 2622 + 0.000 0.444 13.418
ENSG00000185947 E011 323.7154385 0.0002838071 2.113261e-27 2.843401e-25 16 31914476 31917357 2882 + 2.498 2.320 -0.596