ENSG00000185716

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000542527 ENSG00000185716 HEK293_OSMI2_2hA HEK293_TMG_2hB MOSMO protein_coding protein_coding 7.914327 5.243504 10.29844 0.5112219 0.05996724 0.9724749 2.6770548 0.8549886 4.6454756 0.2152156 0.11955335 2.4281755 0.29682500 0.15870000 0.4510667 0.29236667 2.204123e-08 7.483193e-11 FALSE TRUE
ENST00000562695 ENSG00000185716 HEK293_OSMI2_2hA HEK293_TMG_2hB MOSMO protein_coding protein_coding 7.914327 5.243504 10.29844 0.5112219 0.05996724 0.9724749 0.6762805 0.3019605 0.9589066 0.1528418 0.02802028 1.6349942 0.07881667 0.05523333 0.0931000 0.03786667 6.174822e-01 7.483193e-11 FALSE TRUE
MSTRG.12254.6 ENSG00000185716 HEK293_OSMI2_2hA HEK293_TMG_2hB MOSMO protein_coding   7.914327 5.243504 10.29844 0.5112219 0.05996724 0.9724749 3.9548223 3.8372345 4.2838691 0.2521534 0.10306045 0.1584565 0.55479167 0.73606667 0.4159000 -0.32016667 7.483193e-11 7.483193e-11 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185716 E001 1.4706388 0.0672680487 7.404722e-01 8.298889e-01 16 22007638 22007696 59 + 0.346 0.410 0.371
ENSG00000185716 E002 0.4783925 0.0216620095 8.490454e-01 9.063096e-01 16 22008111 22008208 98 + 0.148 0.182 0.362
ENSG00000185716 E003 0.1451727 0.0442257143 1.000000e+00   16 22008209 22008282 74 + 0.080 0.000 -9.762
ENSG00000185716 E004 3.2367338 0.0049593758 9.922540e-01 9.992533e-01 16 22008283 22008310 28 + 0.607 0.616 0.039
ENSG00000185716 E005 38.5021470 0.0006363068 2.963993e-01 4.375966e-01 16 22008311 22008407 97 + 1.545 1.614 0.235
ENSG00000185716 E006 0.2965864 0.0396022264 5.016420e-02   16 22064338 22064438 101 + 0.000 0.309 11.946
ENSG00000185716 E007 0.1515154 0.0441261465 1.000000e+00   16 22075294 22075486 193 + 0.080 0.000 -9.763
ENSG00000185716 E008 72.5572119 0.0004785036 5.119527e-02 1.112808e-01 16 22075487 22075699 213 + 1.810 1.899 0.299
ENSG00000185716 E009 12.3979553 0.0014071061 2.716223e-01 4.103937e-01 16 22075700 22076378 679 + 1.063 1.178 0.415
ENSG00000185716 E010 214.3621173 0.0002902490 1.260725e-41 4.844638e-39 16 22080696 22083675 2980 + 2.383 2.075 -1.029
ENSG00000185716 E011 30.8118094 0.0104141189 8.989776e-01 9.395534e-01 16 22083676 22083868 193 + 1.472 1.503 0.108
ENSG00000185716 E012 55.8102045 0.0320927321 9.148504e-01 9.500705e-01 16 22083869 22084654 786 + 1.712 1.766 0.183
ENSG00000185716 E013 1.2317032 0.0159194586 2.918862e-01 4.327991e-01 16 22085131 22085207 77 + 0.384 0.182 -1.447
ENSG00000185716 E014 2.4054530 0.0061057533 8.175177e-01 8.846231e-01 16 22085208 22085275 68 + 0.510 0.557 0.222
ENSG00000185716 E015 29.6575582 0.0046288639 5.904378e-10 9.138617e-09 16 22085644 22085750 107 + 1.271 1.699 1.472
ENSG00000185716 E016 55.1859279 0.0005295380 1.246876e-25 1.450963e-23 16 22087057 22087534 478 + 1.506 1.989 1.634