Isoforom Switch Analysis
WANG Ziyi
Date: 19 5月 2022
ENSG00000185624
1 Isoform Switch
Summary Plot
Only the isoforms with fraction > 5% are shown below.
Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.
For the function of any other domain, please Click HERE to search it through Pfam database.
Results
| isoform_id | gene_id | condition_1 | condition_2 | gene_name | gene_biotype | iso_biotype | gene_overall_mean | gene_value_1 | gene_value_2 | gene_stderr_1 | gene_stderr_2 | gene_log2_fold_change | iso_overall_mean | iso_value_1 | iso_value_2 | iso_stderr_1 | iso_stderr_2 | iso_log2_fold_change | IF_overall | IF1 | IF2 | dIF | isoform_switch_q_value | gene_switch_q_value | PTC | codingPotential |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ENST00000331483 | ENSG00000185624 | HEK293_OSMI2_2hA | HEK293_TMG_2hB | P4HB | protein_coding | protein_coding | 493.1442 | 676.751 | 423.2354 | 19.48368 | 1.01717 | -0.6771522 | 430.2582 | 567.85445 | 379.264134 | 42.44844 | 3.155169 | -0.5823056 | 0.8689458 | 0.83696667 | 0.896066667 | 0.0591 | 0.450473190 | 3.54573e-11 | FALSE | |
| ENST00000680400 | ENSG00000185624 | HEK293_OSMI2_2hA | HEK293_TMG_2hB | P4HB | protein_coding | protein_coding | 493.1442 | 676.751 | 423.2354 | 19.48368 | 1.01717 | -0.6771522 | 17.6602 | 53.93464 | 3.582904 | 21.09938 | 1.102765 | -3.9082575 | 0.0347500 | 0.08146667 | 0.008466667 | -0.0730 | 0.004158064 | 3.54573e-11 | FALSE |
- Note:
- The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.
2 Differential Exon Usage
Counts
All exons whithin this gene region are shown and numbering below.
Expression
All exons whithin this gene region are shown and numbering below.
Splicing
All exons whithin this gene region are shown and numbering below.
Transcripts
All isoforms whithin this gene region are shown below.
Results
| groupID | featureID | exonBaseMean | dispersion | pvalue | padj | seqnames | start | end | width | strand | HEK293_TMG_2hB | HEK293_OSMI2_2hA | log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ENSG00000185624 | E001 | 6.9812872 | 2.332365e-03 | 2.414474e-01 | 3.758761e-01 | 17 | 81843159 | 81843160 | 2 | - | 0.962 | 0.820 | -0.544 |
| ENSG00000185624 | E002 | 22.0672088 | 3.207113e-03 | 4.372888e-01 | 5.792613e-01 | 17 | 81843161 | 81843162 | 2 | - | 1.375 | 1.313 | -0.214 |
| ENSG00000185624 | E003 | 108.3653545 | 3.721358e-03 | 2.595496e-02 | 6.385499e-02 | 17 | 81843163 | 81843165 | 3 | - | 2.072 | 1.971 | -0.340 |
| ENSG00000185624 | E004 | 205.4947666 | 1.176874e-03 | 9.879567e-05 | 5.162330e-04 | 17 | 81843166 | 81843167 | 2 | - | 2.187 | 2.312 | 0.417 |
| ENSG00000185624 | E005 | 206.5923561 | 1.149645e-03 | 1.145406e-04 | 5.886813e-04 | 17 | 81843168 | 81843168 | 1 | - | 2.191 | 2.314 | 0.411 |
| ENSG00000185624 | E006 | 207.3318329 | 1.141484e-03 | 8.645568e-05 | 4.584423e-04 | 17 | 81843169 | 81843169 | 1 | - | 2.191 | 2.316 | 0.417 |
| ENSG00000185624 | E007 | 218.7177487 | 1.921679e-03 | 2.493650e-05 | 1.512501e-04 | 17 | 81843170 | 81843172 | 3 | - | 2.199 | 2.344 | 0.486 |
| ENSG00000185624 | E008 | 219.4561813 | 1.993403e-03 | 2.756565e-05 | 1.654070e-04 | 17 | 81843173 | 81843173 | 1 | - | 2.200 | 2.346 | 0.486 |
| ENSG00000185624 | E009 | 223.5847715 | 2.075326e-03 | 3.199933e-05 | 1.890858e-04 | 17 | 81843174 | 81843174 | 1 | - | 2.209 | 2.353 | 0.484 |
| ENSG00000185624 | E010 | 223.8062167 | 2.007117e-03 | 2.611777e-05 | 1.576277e-04 | 17 | 81843175 | 81843175 | 1 | - | 2.209 | 2.354 | 0.486 |
| ENSG00000185624 | E011 | 223.9545099 | 2.070998e-03 | 2.820245e-05 | 1.688111e-04 | 17 | 81843176 | 81843176 | 1 | - | 2.209 | 2.354 | 0.487 |
| ENSG00000185624 | E012 | 573.0721175 | 4.194618e-04 | 4.064779e-06 | 2.951326e-05 | 17 | 81843177 | 81843189 | 13 | - | 2.658 | 2.746 | 0.291 |
| ENSG00000185624 | E013 | 580.0728933 | 3.902028e-04 | 2.436068e-06 | 1.859861e-05 | 17 | 81843190 | 81843191 | 2 | - | 2.663 | 2.751 | 0.293 |
| ENSG00000185624 | E014 | 595.8632419 | 4.204028e-04 | 2.159907e-07 | 2.056944e-06 | 17 | 81843192 | 81843193 | 2 | - | 2.668 | 2.765 | 0.324 |
| ENSG00000185624 | E015 | 719.4826011 | 1.974327e-03 | 4.353773e-06 | 3.141054e-05 | 17 | 81843194 | 81843194 | 1 | - | 2.731 | 2.853 | 0.409 |
| ENSG00000185624 | E016 | 790.3300807 | 2.413509e-03 | 7.268639e-07 | 6.220735e-06 | 17 | 81843195 | 81843198 | 4 | - | 2.757 | 2.898 | 0.470 |
| ENSG00000185624 | E017 | 830.7242253 | 2.526944e-03 | 1.373483e-07 | 1.358971e-06 | 17 | 81843199 | 81843201 | 3 | - | 2.770 | 2.923 | 0.506 |
| ENSG00000185624 | E018 | 867.5070585 | 2.432854e-03 | 5.879553e-08 | 6.241881e-07 | 17 | 81843202 | 81843203 | 2 | - | 2.788 | 2.942 | 0.511 |
| ENSG00000185624 | E019 | 883.1143151 | 2.308944e-03 | 4.234974e-08 | 4.635179e-07 | 17 | 81843204 | 81843206 | 3 | - | 2.797 | 2.949 | 0.504 |
| ENSG00000185624 | E020 | 970.3177249 | 2.401477e-03 | 2.304959e-09 | 3.195987e-08 | 17 | 81843207 | 81843208 | 2 | - | 2.827 | 2.993 | 0.553 |
| ENSG00000185624 | E021 | 992.0568994 | 2.667860e-03 | 6.036916e-09 | 7.765842e-08 | 17 | 81843209 | 81843211 | 3 | - | 2.835 | 3.003 | 0.560 |
| ENSG00000185624 | E022 | 1027.5451789 | 2.379335e-03 | 5.626538e-11 | 1.035268e-09 | 17 | 81843212 | 81843216 | 5 | - | 2.840 | 3.021 | 0.601 |
| ENSG00000185624 | E023 | 1431.0128812 | 1.682366e-03 | 5.215666e-13 | 1.338516e-11 | 17 | 81843217 | 81843230 | 14 | - | 2.995 | 3.162 | 0.553 |
| ENSG00000185624 | E024 | 1568.5363651 | 1.363631e-03 | 2.476345e-11 | 4.838950e-10 | 17 | 81843231 | 81843237 | 7 | - | 3.056 | 3.195 | 0.464 |
| ENSG00000185624 | E025 | 2445.4334533 | 2.217230e-03 | 1.017628e-09 | 1.507440e-08 | 17 | 81843238 | 81843266 | 29 | - | 3.239 | 3.390 | 0.501 |
| ENSG00000185624 | E026 | 3594.4395505 | 2.513806e-03 | 1.506880e-08 | 1.797241e-07 | 17 | 81843267 | 81843330 | 64 | - | 3.411 | 3.556 | 0.481 |
| ENSG00000185624 | E027 | 2401.1732008 | 2.894558e-03 | 8.958739e-06 | 6.023161e-05 | 17 | 81843331 | 81843333 | 3 | - | 3.252 | 3.376 | 0.413 |
| ENSG00000185624 | E028 | 3369.0722497 | 2.443950e-03 | 8.424964e-08 | 8.679088e-07 | 17 | 81843334 | 81843363 | 30 | - | 3.390 | 3.526 | 0.451 |
| ENSG00000185624 | E029 | 4271.7591186 | 2.643780e-03 | 1.841380e-08 | 2.162434e-07 | 17 | 81843364 | 81843421 | 58 | - | 3.485 | 3.631 | 0.485 |
| ENSG00000185624 | E030 | 2927.0825904 | 2.436245e-03 | 2.766370e-08 | 3.136517e-07 | 17 | 81843422 | 81843430 | 9 | - | 3.324 | 3.466 | 0.471 |
| ENSG00000185624 | E031 | 4701.8371981 | 1.512536e-03 | 1.399993e-12 | 3.347949e-11 | 17 | 81843431 | 81843513 | 83 | - | 3.531 | 3.671 | 0.465 |
| ENSG00000185624 | E032 | 2578.0954933 | 1.199793e-03 | 6.007731e-12 | 1.296295e-10 | 17 | 81843514 | 81843521 | 8 | - | 3.279 | 3.408 | 0.428 |
| ENSG00000185624 | E033 | 9322.7116441 | 1.232003e-04 | 3.237502e-49 | 1.869924e-46 | 17 | 81843522 | 81843878 | 357 | - | 3.862 | 3.957 | 0.316 |
| ENSG00000185624 | E034 | 5173.5454415 | 5.819787e-04 | 3.548067e-03 | 1.186281e-02 | 17 | 81843879 | 81844040 | 162 | - | 3.650 | 3.685 | 0.119 |
| ENSG00000185624 | E035 | 3539.9884758 | 6.127884e-04 | 1.180749e-01 | 2.171750e-01 | 17 | 81844041 | 81844092 | 52 | - | 3.496 | 3.516 | 0.066 |
| ENSG00000185624 | E036 | 107.4242472 | 1.137642e-03 | 1.959672e-01 | 3.211701e-01 | 17 | 81844093 | 81845143 | 1051 | - | 2.032 | 1.980 | -0.174 |
| ENSG00000185624 | E037 | 4680.3031121 | 2.076131e-04 | 2.372292e-01 | 3.709738e-01 | 17 | 81845144 | 81845230 | 87 | - | 3.640 | 3.628 | -0.039 |
| ENSG00000185624 | E038 | 399.4010637 | 1.341900e-04 | 7.176607e-01 | 8.129651e-01 | 17 | 81845231 | 81845303 | 73 | - | 2.570 | 2.562 | -0.027 |
| ENSG00000185624 | E039 | 98.0913652 | 1.582463e-03 | 5.404452e-05 | 3.019735e-04 | 17 | 81845304 | 81845459 | 156 | - | 2.065 | 1.898 | -0.562 |
| ENSG00000185624 | E040 | 105.8603193 | 2.932995e-04 | 1.136804e-02 | 3.204442e-02 | 17 | 81845460 | 81845560 | 101 | - | 2.053 | 1.961 | -0.312 |
| ENSG00000185624 | E041 | 3687.4051767 | 1.116270e-04 | 2.216963e-03 | 7.896313e-03 | 17 | 81845561 | 81845626 | 66 | - | 3.546 | 3.520 | -0.085 |
| ENSG00000185624 | E042 | 4763.9090388 | 8.704284e-05 | 7.208064e-05 | 3.902898e-04 | 17 | 81845627 | 81845742 | 116 | - | 3.659 | 3.630 | -0.096 |
| ENSG00000185624 | E043 | 20.8789828 | 1.857575e-02 | 2.360035e-01 | 3.695451e-01 | 17 | 81845743 | 81845817 | 75 | - | 1.375 | 1.256 | -0.415 |
| ENSG00000185624 | E044 | 19.9365266 | 6.716215e-03 | 5.580137e-01 | 6.863210e-01 | 17 | 81845818 | 81845870 | 53 | - | 1.239 | 1.291 | 0.181 |
| ENSG00000185624 | E045 | 3576.6660330 | 4.211981e-04 | 9.808913e-01 | 9.920557e-01 | 17 | 81845871 | 81845946 | 76 | - | 3.516 | 3.514 | -0.006 |
| ENSG00000185624 | E046 | 3779.0622480 | 3.525638e-04 | 4.622193e-03 | 1.488080e-02 | 17 | 81845947 | 81845991 | 45 | - | 3.561 | 3.529 | -0.108 |
| ENSG00000185624 | E047 | 20.2538065 | 1.044217e-03 | 9.244245e-01 | 9.563957e-01 | 17 | 81845992 | 81846010 | 19 | - | 1.282 | 1.289 | 0.023 |
| ENSG00000185624 | E048 | 13.7739638 | 6.504054e-02 | 8.955276e-01 | 9.372532e-01 | 17 | 81846011 | 81846359 | 349 | - | 1.106 | 1.133 | 0.096 |
| ENSG00000185624 | E049 | 4.9986680 | 1.742470e-02 | 4.565856e-01 | 5.969812e-01 | 17 | 81846360 | 81846428 | 69 | - | 0.809 | 0.698 | -0.448 |
| ENSG00000185624 | E050 | 2841.5628622 | 3.265143e-04 | 1.705798e-04 | 8.375664e-04 | 17 | 81846429 | 81846430 | 2 | - | 3.445 | 3.401 | -0.146 |
| ENSG00000185624 | E051 | 3010.1853363 | 5.390341e-04 | 1.027152e-03 | 4.052373e-03 | 17 | 81846431 | 81846442 | 12 | - | 3.471 | 3.426 | -0.150 |
| ENSG00000185624 | E052 | 3208.7251145 | 3.632649e-04 | 5.685304e-05 | 3.158175e-04 | 17 | 81846443 | 81846469 | 27 | - | 3.500 | 3.453 | -0.157 |
| ENSG00000185624 | E053 | 3955.1876146 | 6.030936e-04 | 2.463630e-07 | 2.320410e-06 | 17 | 81846470 | 81846509 | 40 | - | 3.606 | 3.536 | -0.233 |
| ENSG00000185624 | E054 | 5910.2636474 | 4.972475e-04 | 4.544015e-14 | 1.355898e-12 | 17 | 81846510 | 81846629 | 120 | - | 3.794 | 3.704 | -0.297 |
| ENSG00000185624 | E055 | 20.1578302 | 9.320958e-04 | 3.642791e-01 | 5.086461e-01 | 17 | 81846630 | 81846946 | 317 | - | 1.340 | 1.267 | -0.254 |
| ENSG00000185624 | E056 | 4289.6887010 | 8.042660e-04 | 1.122855e-09 | 1.649260e-08 | 17 | 81846947 | 81847012 | 66 | - | 3.656 | 3.564 | -0.305 |
| ENSG00000185624 | E057 | 3821.8893248 | 8.054224e-04 | 1.602490e-12 | 3.794837e-11 | 17 | 81847013 | 81847068 | 56 | - | 3.616 | 3.509 | -0.357 |
| ENSG00000185624 | E058 | 2424.3926574 | 3.817062e-04 | 7.701377e-16 | 2.953447e-14 | 17 | 81847069 | 81847072 | 4 | - | 3.413 | 3.314 | -0.329 |
| ENSG00000185624 | E059 | 4768.8397144 | 7.224276e-04 | 9.653446e-15 | 3.182764e-13 | 17 | 81847243 | 81847347 | 105 | - | 3.714 | 3.604 | -0.365 |
| ENSG00000185624 | E060 | 8.1529701 | 4.597634e-03 | 8.523575e-01 | 9.085090e-01 | 17 | 81847348 | 81847371 | 24 | - | 0.917 | 0.939 | 0.083 |
| ENSG00000185624 | E061 | 8.9903861 | 1.954453e-03 | 1.992482e-02 | 5.132920e-02 | 17 | 81847372 | 81848528 | 1157 | - | 0.743 | 1.033 | 1.111 |
| ENSG00000185624 | E062 | 0.6966155 | 1.726718e-02 | 7.033284e-01 | 8.020191e-01 | 17 | 81853247 | 81853280 | 34 | - | 0.162 | 0.231 | 0.626 |
| ENSG00000185624 | E063 | 0.9900813 | 1.399636e-02 | 9.725492e-01 | 9.868732e-01 | 17 | 81853281 | 81853403 | 123 | - | 0.280 | 0.286 | 0.040 |
| ENSG00000185624 | E064 | 1.3920981 | 1.605581e-02 | 1.700228e-01 | 2.882200e-01 | 17 | 81854894 | 81855027 | 134 | - | 0.163 | 0.421 | 1.848 |
| ENSG00000185624 | E065 | 4363.2860710 | 1.607997e-03 | 3.044896e-07 | 2.813610e-06 | 17 | 81855142 | 81855279 | 138 | - | 3.672 | 3.567 | -0.348 |
| ENSG00000185624 | E066 | 14.9864387 | 1.185287e-03 | 7.788525e-01 | 8.577415e-01 | 17 | 81855280 | 81855452 | 173 | - | 1.151 | 1.176 | 0.089 |
| ENSG00000185624 | E067 | 2370.2691302 | 2.455043e-03 | 3.681686e-03 | 1.224323e-02 | 17 | 81855453 | 81855505 | 53 | - | 3.388 | 3.312 | -0.251 |
| ENSG00000185624 | E068 | 2764.1950678 | 1.976216e-03 | 8.819582e-04 | 3.551025e-03 | 17 | 81855506 | 81855579 | 74 | - | 3.456 | 3.378 | -0.257 |
| ENSG00000185624 | E069 | 1704.2044927 | 1.541497e-03 | 2.880211e-06 | 2.163464e-05 | 17 | 81855580 | 81855586 | 7 | - | 3.261 | 3.160 | -0.337 |
| ENSG00000185624 | E070 | 18.9937926 | 9.480699e-04 | 8.825286e-01 | 9.288002e-01 | 17 | 81855587 | 81855688 | 102 | - | 1.261 | 1.273 | 0.040 |
| ENSG00000185624 | E071 | 111.3950853 | 1.379057e-03 | 1.152008e-07 | 1.157304e-06 | 17 | 81855689 | 81858494 | 2806 | - | 2.143 | 1.937 | -0.689 |
| ENSG00000185624 | E072 | 28.3093098 | 2.611617e-02 | 3.735598e-02 | 8.614073e-02 | 17 | 81858495 | 81858934 | 440 | - | 1.562 | 1.350 | -0.729 |
| ENSG00000185624 | E073 | 33.4374228 | 6.069795e-04 | 8.998912e-04 | 3.613492e-03 | 17 | 81858935 | 81859178 | 244 | - | 1.631 | 1.426 | -0.701 |
| ENSG00000185624 | E074 | 26.8466142 | 4.569967e-03 | 7.185481e-01 | 8.136201e-01 | 17 | 81859179 | 81859180 | 2 | - | 1.422 | 1.392 | -0.103 |
| ENSG00000185624 | E075 | 3162.6504190 | 1.153385e-03 | 9.579075e-08 | 9.759746e-07 | 17 | 81859181 | 81859289 | 109 | - | 3.527 | 3.430 | -0.321 |
| ENSG00000185624 | E076 | 2440.5931825 | 1.508704e-03 | 3.028809e-08 | 3.406143e-07 | 17 | 81859290 | 81859346 | 57 | - | 3.426 | 3.311 | -0.381 |
| ENSG00000185624 | E077 | 1901.3922580 | 2.841125e-03 | 3.948750e-07 | 3.563807e-06 | 17 | 81859347 | 81859387 | 41 | - | 3.334 | 3.194 | -0.467 |
| ENSG00000185624 | E078 | 99.2211475 | 3.153449e-04 | 2.186316e-19 | 1.306184e-17 | 17 | 81859388 | 81859766 | 379 | - | 2.162 | 1.835 | -1.097 |
| ENSG00000185624 | E079 | 31.2754617 | 6.227854e-04 | 2.010476e-06 | 1.563284e-05 | 17 | 81859767 | 81859810 | 44 | - | 1.658 | 1.361 | -1.020 |
| ENSG00000185624 | E080 | 30.5703094 | 6.419331e-04 | 4.064562e-05 | 2.338565e-04 | 17 | 81859811 | 81859841 | 31 | - | 1.626 | 1.365 | -0.897 |
| ENSG00000185624 | E081 | 32.7084069 | 6.333166e-04 | 4.039478e-06 | 2.934981e-05 | 17 | 81859842 | 81859884 | 43 | - | 1.666 | 1.382 | -0.974 |
| ENSG00000185624 | E082 | 63.6605433 | 4.415531e-04 | 4.301091e-08 | 4.701569e-07 | 17 | 81859885 | 81860326 | 442 | - | 1.929 | 1.681 | -0.839 |
| ENSG00000185624 | E083 | 1239.5228261 | 4.521350e-03 | 2.566726e-05 | 1.551707e-04 | 17 | 81860327 | 81860331 | 5 | - | 3.152 | 3.006 | -0.488 |
| ENSG00000185624 | E084 | 1155.4680049 | 5.157004e-03 | 3.088664e-05 | 1.832221e-04 | 17 | 81860332 | 81860336 | 5 | - | 3.127 | 2.972 | -0.514 |
| ENSG00000185624 | E085 | 1270.8149372 | 4.781840e-03 | 2.278734e-06 | 1.750463e-05 | 17 | 81860337 | 81860346 | 10 | - | 3.177 | 3.008 | -0.561 |
| ENSG00000185624 | E086 | 2147.3413324 | 7.853544e-03 | 3.599777e-06 | 2.647696e-05 | 17 | 81860347 | 81860624 | 278 | - | 3.426 | 3.222 | -0.677 |
| ENSG00000185624 | E087 | 21.9991472 | 2.255663e-02 | 3.490573e-01 | 4.932890e-01 | 17 | 81860699 | 81860856 | 158 | - | 1.383 | 1.286 | -0.337 |