ENSG00000185532

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373980 ENSG00000185532 HEK293_OSMI2_2hA HEK293_TMG_2hB PRKG1 protein_coding protein_coding 2.577085 0.8903135 5.853287 0.1347083 0.1307777 2.70321 0.43803803 0.14339842 0.877759764 0.08617926 0.109967563 2.532886 0.22082500 0.1757000 0.149966667 -0.02573333 1.000000e+00 4.469102e-05 FALSE TRUE
ENST00000401604 ENSG00000185532 HEK293_OSMI2_2hA HEK293_TMG_2hB PRKG1 protein_coding protein_coding 2.577085 0.8903135 5.853287 0.1347083 0.1307777 2.70321 0.90828205 0.09893751 2.191538937 0.06562606 0.047439166 4.336940 0.28304583 0.1059333 0.374433333 0.26850000 1.188533e-03 4.469102e-05 FALSE TRUE
ENST00000645324 ENSG00000185532 HEK293_OSMI2_2hA HEK293_TMG_2hB PRKG1 protein_coding protein_coding 2.577085 0.8903135 5.853287 0.1347083 0.1307777 2.70321 0.22263095 0.13825888 0.382771371 0.13825888 0.121222898 1.405571 0.12117083 0.1255667 0.066333333 -0.05923333 9.358234e-01 4.469102e-05 FALSE TRUE
ENST00000645790 ENSG00000185532 HEK293_OSMI2_2hA HEK293_TMG_2hB PRKG1 protein_coding processed_transcript 2.577085 0.8903135 5.853287 0.1347083 0.1307777 2.70321 0.45351213 0.27078096 1.268312464 0.08764713 0.155396200 2.186723 0.16345417 0.2861333 0.215833333 -0.07030000 6.244505e-01 4.469102e-05   FALSE
MSTRG.3986.1 ENSG00000185532 HEK293_OSMI2_2hA HEK293_TMG_2hB PRKG1 protein_coding   2.577085 0.8903135 5.853287 0.1347083 0.1307777 2.70321 0.09419132 0.19122603 0.008323883 0.07129529 0.008323883 -3.457020 0.07765417 0.2411000 0.001366667 -0.23973333 5.939240e-05 4.469102e-05 TRUE TRUE
MSTRG.3986.7 ENSG00000185532 HEK293_OSMI2_2hA HEK293_TMG_2hB PRKG1 protein_coding   2.577085 0.8903135 5.853287 0.1347083 0.1307777 2.70321 0.40030708 0.00000000 1.028984953 0.00000000 0.072876703 6.699031 0.09009167 0.0000000 0.176133333 0.17613333 4.469102e-05 4.469102e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185532 E001 0.1451727 0.0431463527 6.492988e-01   10 50990536 50990887 352 + 0.042 0.000 -6.531
ENSG00000185532 E002 21.2858899 0.0014040914 2.123076e-01 3.412566e-01 10 50990888 50991644 757 + 1.122 1.317 0.688
ENSG00000185532 E003 1.5373620 0.0428976704 3.744207e-01 5.188353e-01 10 51074304 51074486 183 + 0.259 0.488 1.350
ENSG00000185532 E004 2.1295923 0.0079017671 2.276927e-01 3.596500e-01 10 51074487 51074509 23 + 0.327 0.614 1.473
ENSG00000185532 E005 4.6437735 0.0036418396 1.188141e-01 2.182528e-01 10 51074510 51074569 60 + 0.538 0.859 1.348
ENSG00000185532 E006 10.2517200 0.0295718584 4.350864e-02 9.748420e-02 10 51074570 51074901 332 + 0.807 1.163 1.328
ENSG00000185532 E007 25.8359855 0.0008653067 3.632144e-02 8.419221e-02 10 51153164 51153330 167 + 1.190 1.447 0.898
ENSG00000185532 E008 13.9930048 0.0014071534 4.070884e-01 5.507006e-01 10 51467723 51467836 114 + 0.962 1.129 0.610
ENSG00000185532 E009 8.4119203 0.0027441721 3.003056e-02 7.201975e-02 10 51568426 51568789 364 + 0.731 1.094 1.381
ENSG00000185532 E010 17.6413558 0.0095141392 9.827047e-02 1.879010e-01 10 51804585 51804690 106 + 1.041 1.294 0.903
ENSG00000185532 E011 13.2740948 0.0361641618 1.468401e-01 2.573801e-01 10 51907507 51907570 64 + 0.926 1.192 0.971
ENSG00000185532 E012 0.0000000       10 51907571 51908754 1184 +      
ENSG00000185532 E013 0.5085815 0.3487352723 4.070392e-02 9.232802e-02 10 51908755 51911205 2451 + 0.042 0.490 4.360
ENSG00000185532 E014 0.0000000       10 52046748 52046866 119 +      
ENSG00000185532 E015 0.0000000       10 52046867 52046926 60 +      
ENSG00000185532 E016 16.4717627 0.0065679471 6.767342e-01 7.816202e-01 10 52054484 52054561 78 + 1.049 1.056 0.025
ENSG00000185532 E017 14.7942510 0.0283493252 3.858098e-02 8.841642e-02 10 52062537 52062631 95 + 1.032 0.711 -1.238
ENSG00000185532 E018 0.2934659 0.0293548378 1.205312e-01   10 52062632 52062787 156 + 0.042 0.309 3.345
ENSG00000185532 E019 0.2924217 0.0275451944 1.000000e+00   10 52062788 52062862 75 + 0.081 0.000 -9.413
ENSG00000185532 E020 0.0000000       10 52081597 52081657 61 +      
ENSG00000185532 E021 0.2987644 0.0276636484 1.000000e+00   10 52125734 52125983 250 + 0.081 0.000 -9.413
ENSG00000185532 E022 0.0000000       10 52128346 52128369 24 +      
ENSG00000185532 E023 0.1472490 0.0443755766 6.509903e-01   10 52128370 52128541 172 + 0.042 0.000 -8.399
ENSG00000185532 E024 11.5975456 0.0015507385 3.223816e-02 7.630941e-02 10 52133840 52133905 66 + 0.937 0.614 -1.297
ENSG00000185532 E025 11.6090732 0.0015049039 7.640239e-03 2.287399e-02 10 52161889 52161963 75 + 0.942 0.488 -1.901
ENSG00000185532 E026 0.0000000       10 52184688 52185074 387 +      
ENSG00000185532 E027 11.1460618 0.0183502444 2.414914e-03 8.506690e-03 10 52251570 52251666 97 + 0.932 0.309 -2.866
ENSG00000185532 E028 13.4832575 0.0014062777 1.067133e-02 3.038569e-02 10 52271350 52271489 140 + 0.999 0.614 -1.528
ENSG00000185532 E029 11.1151649 0.0046664515 1.076710e-02 3.060823e-02 10 52272392 52272481 90 + 0.927 0.488 -1.844
ENSG00000185532 E030 13.0296271 0.0078238924 1.337824e-01 2.394124e-01 10 52280789 52280930 142 + 0.972 0.792 -0.689
ENSG00000185532 E031 16.5645016 0.0010660360 1.796600e-03 6.588600e-03 10 52282153 52282316 164 + 1.080 0.614 -1.823
ENSG00000185532 E032 16.6961722 0.0012413312 2.852045e-04 1.319667e-03 10 52288726 52288848 123 + 1.087 0.488 -2.434
ENSG00000185532 E033 10.4468750 0.0017051020 2.485571e-03 8.721203e-03 10 52288931 52288993 63 + 0.905 0.309 -2.761
ENSG00000185532 E034 10.1514716 0.0138922025 2.531748e-02 6.252557e-02 10 52290224 52290290 67 + 0.889 0.488 -1.697
ENSG00000185532 E035 41.7044397 0.0005667172 1.413210e-05 9.065706e-05 10 52293802 52294568 767 + 1.454 1.056 -1.402
ENSG00000185532 E036 93.8481216 0.0003420697 6.900459e-04 2.862867e-03 10 52294569 52296704 2136 + 1.780 1.649 -0.443
ENSG00000185532 E037 87.7140186 0.0207594936 2.703882e-08 3.071550e-07 10 52296705 52298423 1719 + 1.661 2.119 1.545