ENSG00000185495

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000540997 ENSG00000185495 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene retained_intron 6.078335 6.386285 5.781712 0.4662119 0.6656108 -0.1432442 1.21180023 0.25628680 1.27331150 0.085701291 0.15688029 2.268819 0.19920417 0.04103333 0.22420000 0.18316667 5.898863e-05 2.304016e-10 FALSE FALSE
ENST00000572124 ENSG00000185495 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene retained_intron 6.078335 6.386285 5.781712 0.4662119 0.6656108 -0.1432442 0.54173002 0.07783107 0.85407629 0.009989989 0.14786517 3.298355 0.09307083 0.01230000 0.14560000 0.13330000 2.304016e-10 2.304016e-10   FALSE
ENST00000622033 ENSG00000185495 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 6.078335 6.386285 5.781712 0.4662119 0.6656108 -0.1432442 0.34243493 0.00000000 0.52189478 0.000000000 0.19208001 5.733069 0.05710417 0.00000000 0.08430000 0.08430000 1.865009e-05 2.304016e-10   FALSE
MSTRG.3121.1 ENSG00000185495 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene   6.078335 6.386285 5.781712 0.4662119 0.6656108 -0.1432442 0.25662687 0.13562426 0.62008323 0.089196738 0.27598463 2.113292 0.04284167 0.02286667 0.09986667 0.07700000 2.935633e-01 2.304016e-10   FALSE
MSTRG.3121.10 ENSG00000185495 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene   6.078335 6.386285 5.781712 0.4662119 0.6656108 -0.1432442 0.09358149 0.00000000 0.40559936 0.000000000 0.40559936 5.377122 0.01568333 0.00000000 0.06526667 0.06526667 8.148542e-01 2.304016e-10   FALSE
MSTRG.3121.2 ENSG00000185495 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene   6.078335 6.386285 5.781712 0.4662119 0.6656108 -0.1432442 2.15428513 3.96124469 0.77461224 0.536007440 0.39059924 -2.339540 0.35275833 0.62753333 0.14263333 -0.48490000 1.792979e-01 2.304016e-10   FALSE
MSTRG.3121.5 ENSG00000185495 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene   6.078335 6.386285 5.781712 0.4662119 0.6656108 -0.1432442 0.54181378 0.30432571 0.88859985 0.061653236 0.06147633 1.515419 0.09206667 0.04726667 0.15790000 0.11063333 6.382790e-04 2.304016e-10   FALSE
MSTRG.3121.7 ENSG00000185495 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene   6.078335 6.386285 5.781712 0.4662119 0.6656108 -0.1432442 0.51577973 1.12445636 0.04669967 0.676465015 0.04669967 -4.322517 0.07864583 0.16470000 0.01043333 -0.15426667 3.847117e-01 2.304016e-10   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185495 E001 1.296320 0.1025171444 6.725297e-01 7.784775e-01 1 223992657 223992742 86 + 0.293 0.371 0.482
ENSG00000185495 E002 1.626317 0.0112705698 2.893435e-01 4.300316e-01 1 223992743 223992754 12 + 0.293 0.475 1.041
ENSG00000185495 E003 2.332498 0.1051134139 3.875139e-01 5.316572e-01 1 223992755 223992763 9 + 0.417 0.568 0.745
ENSG00000185495 E004 13.703753 0.0022665259 2.380853e-01 3.719165e-01 1 223992764 223992962 199 + 1.099 1.200 0.362
ENSG00000185495 E005 16.468300 0.0052839318 2.259407e-01 3.575206e-01 1 223992963 223993047 85 + 1.189 1.288 0.351
ENSG00000185495 E006 4.146630 0.0383155253 2.825872e-02 6.851993e-02 1 223995895 223995956 62 + 0.871 0.518 -1.484
ENSG00000185495 E007 5.835607 0.0033147541 6.546824e-03 2.004681e-02 1 223995957 223996001 45 + 0.985 0.633 -1.394
ENSG00000185495 E008 5.647584 0.0030256479 2.473422e-02 6.132956e-02 1 223996002 223996094 93 + 0.956 0.665 -1.146
ENSG00000185495 E009 1.978671 0.0506756135 3.425904e-03 1.151338e-02 1 223998106 223998158 53 + 0.687 0.124 -3.548
ENSG00000185495 E010 9.103195 0.0046727827 2.817723e-15 1.006048e-13 1 224001907 224002521 615 + 1.300 0.220 -4.844
ENSG00000185495 E011 10.188381 0.0018541300 1.581700e-04 7.835788e-04 1 224002522 224002622 101 + 1.215 0.820 -1.459
ENSG00000185495 E012 5.738748 0.0030702020 2.200863e-03 7.844573e-03 1 224002623 224003519 897 + 1.000 0.599 -1.599
ENSG00000185495 E013 35.965649 0.0007144416 1.792300e-01 3.001599e-01 1 224003520 224003654 135 + 1.528 1.597 0.236
ENSG00000185495 E014 1.678761 0.0105039756 3.617129e-02 8.391657e-02 1 224004707 224004724 18 + 0.590 0.220 -2.133
ENSG00000185495 E015 2.535399 0.0157086847 1.549466e-02 4.161705e-02 1 224004725 224004792 68 + 0.714 0.299 -2.076
ENSG00000185495 E016 7.910716 0.0022323679 9.949129e-10 1.476447e-08 1 224007294 224007417 124 + 1.207 0.423 -3.194
ENSG00000185495 E017 13.110288 0.0014698155 7.637545e-12 1.621006e-10 1 224008727 224009319 593 + 1.388 0.695 -2.566
ENSG00000185495 E018 38.799307 0.0006154720 4.779182e-01 6.163870e-01 1 224009320 224009446 127 + 1.579 1.612 0.112
ENSG00000185495 E019 79.041664 0.0003885287 2.656848e-09 3.643108e-08 1 224009447 224010288 842 + 1.781 1.984 0.682
ENSG00000185495 E020 27.036209 0.0159269368 5.282188e-11 9.766686e-10 1 224010289 224010615 327 + 1.027 1.633 2.122