ENSG00000185418

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000335968 ENSG00000185418 HEK293_OSMI2_2hA HEK293_TMG_2hB TARS3 protein_coding protein_coding 14.43203 9.451805 21.16505 0.3091572 0.9341183 1.162178 7.697469 6.632454 11.101125 0.3279872 0.7152424 0.7422168 0.5593292 0.7012333 0.5280 -0.1732333 4.279612e-02 1.613301e-16 FALSE TRUE
ENST00000558533 ENSG00000185418 HEK293_OSMI2_2hA HEK293_TMG_2hB TARS3 protein_coding nonsense_mediated_decay 14.43203 9.451805 21.16505 0.3091572 0.9341183 1.162178 2.714072 2.128204 2.479849 0.3838854 0.1917935 0.2196590 0.1979500 0.2259000 0.1184 -0.1075000 1.116184e-01 1.613301e-16 TRUE TRUE
ENST00000615656 ENSG00000185418 HEK293_OSMI2_2hA HEK293_TMG_2hB TARS3 protein_coding protein_coding 14.43203 9.451805 21.16505 0.3091572 0.9341183 1.162178 2.672018 0.000000 5.897588 0.0000000 1.3237963 9.2064253 0.1407458 0.0000000 0.2745 0.2745000 1.613301e-16 1.613301e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185418 E001 1.0404339 0.0116728233 2.115624e-01 3.403376e-01 15 101653503 101653595 93 - 0.387 0.159 -1.700
ENSG00000185418 E002 64.6903451 0.0061846862 4.371800e-05 2.498038e-04 15 101653596 101654212 617 - 1.700 1.945 0.828
ENSG00000185418 E003 137.4920805 0.0030011960 5.517817e-04 2.353561e-03 15 101654213 101654393 181 - 2.071 2.215 0.480
ENSG00000185418 E004 123.3431394 0.0005844912 3.051476e-04 1.400351e-03 15 101654394 101654468 75 - 2.032 2.160 0.428
ENSG00000185418 E005 227.6792728 0.0039655863 8.406993e-06 5.682203e-05 15 101654469 101654730 262 - 2.275 2.442 0.558
ENSG00000185418 E006 1.1352540 0.0680408768 2.727417e-01 4.116973e-01 15 101655867 101655979 113 - 0.235 0.442 1.300
ENSG00000185418 E007 1.1352540 0.0680408768 2.727417e-01 4.116973e-01 15 101655980 101656020 41 - 0.235 0.442 1.300
ENSG00000185418 E008 123.0449415 0.0005558745 2.038379e-04 9.803130e-04 15 101656922 101657036 115 - 2.029 2.159 0.438
ENSG00000185418 E009 85.3735117 0.0003663748 1.158042e-01 2.139532e-01 15 101657785 101657857 73 - 1.899 1.969 0.233
ENSG00000185418 E010 106.3694146 0.0006769607 5.373778e-02 1.157937e-01 15 101661712 101661816 105 - 1.994 2.072 0.261
ENSG00000185418 E011 2.5079548 0.0060400001 2.364272e-04 1.118363e-03 15 101664170 101664442 273 - 0.705 0.000 -12.607
ENSG00000185418 E012 90.2260963 0.0048992518 4.967398e-01 6.333543e-01 15 101671486 101671586 101 - 1.937 1.981 0.150
ENSG00000185418 E013 97.6888105 0.0029349598 6.616997e-01 7.701387e-01 15 101671671 101671748 78 - 1.978 2.009 0.103
ENSG00000185418 E014 127.7219537 0.0002882234 1.948885e-01 3.198566e-01 15 101675600 101675737 138 - 2.116 2.084 -0.109
ENSG00000185418 E015 148.9746127 0.0002897893 1.359189e-02 3.728721e-02 15 101684075 101684237 163 - 2.194 2.129 -0.218
ENSG00000185418 E016 164.4414669 0.0002191244 1.457740e-01 2.559092e-01 15 101685896 101686062 167 - 2.224 2.191 -0.108
ENSG00000185418 E017 0.8417882 0.0163310428 2.720402e-01 4.108777e-01 15 101695760 101696039 280 - 0.170 0.367 1.472
ENSG00000185418 E018 7.7167778 0.0327996007 3.340531e-01 4.778164e-01 15 101699331 101699435 105 - 0.859 0.999 0.527
ENSG00000185418 E019 98.4780246 0.0003414383 6.835752e-02 1.406522e-01 15 101701086 101701184 99 - 2.011 1.952 -0.198
ENSG00000185418 E020 122.0746778 0.0009795111 6.560977e-02 1.360594e-01 15 101702239 101702385 147 - 2.103 2.046 -0.193
ENSG00000185418 E021 83.1733162 0.0026172548 7.546351e-02 1.522581e-01 15 101703859 101703937 79 - 1.944 1.867 -0.258
ENSG00000185418 E022 72.7491977 0.0017494963 2.861224e-02 6.923267e-02 15 101705683 101705747 65 - 1.894 1.799 -0.319
ENSG00000185418 E023 91.4559241 0.0040903659 1.600060e-01 2.751294e-01 15 101708793 101708910 118 - 1.980 1.917 -0.213
ENSG00000185418 E024 94.5612399 0.0028396010 1.734959e-01 2.927401e-01 15 101711880 101712001 122 - 1.992 1.937 -0.185
ENSG00000185418 E025 1.4371521 0.0114181221 2.304796e-01 3.630259e-01 15 101714678 101714839 162 - 0.292 0.507 1.207
ENSG00000185418 E026 93.3628582 0.0065012295 8.185126e-02 1.624616e-01 15 101714840 101714963 124 - 1.998 1.908 -0.304
ENSG00000185418 E027 117.6925634 0.0003189779 1.529006e-04 7.599383e-04 15 101721126 101721322 197 - 2.110 1.989 -0.406
ENSG00000185418 E028 53.7504814 0.0026910016 8.318153e-05 4.430650e-04 15 101723093 101723164 72 - 1.803 1.586 -0.733
ENSG00000185418 E029 75.9442207 0.0015418580 6.615765e-13 1.672677e-11 15 101724091 101724473 383 - 1.981 1.638 -1.158