ENSG00000185246

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000355765 ENSG00000185246 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF39 protein_coding protein_coding 20.3731 4.667238 33.14339 0.4358627 0.5267264 2.825428 2.7608357 1.2562954 4.4596211 0.3132668 0.6278631 1.8195386 0.15194167 0.26053333 0.13410000 -0.12643333 1.227224e-01 1.776251e-08 FALSE TRUE
ENST00000424478 ENSG00000185246 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF39 protein_coding nonsense_mediated_decay 20.3731 4.667238 33.14339 0.4358627 0.5267264 2.825428 1.7643362 0.1831184 3.2621085 0.1051614 0.4957626 4.0826628 0.08248333 0.03586667 0.09890000 0.06303333 2.335856e-01 1.776251e-08 FALSE TRUE
ENST00000477626 ENSG00000185246 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF39 protein_coding retained_intron 20.3731 4.667238 33.14339 0.4358627 0.5267264 2.825428 4.4814205 0.5378773 7.1992378 0.1640296 0.3463010 3.7179219 0.20469167 0.11416667 0.21730000 0.10313333 6.219066e-02 1.776251e-08 FALSE TRUE
ENST00000553605 ENSG00000185246 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF39 protein_coding protein_coding 20.3731 4.667238 33.14339 0.4358627 0.5267264 2.825428 1.8966790 0.0000000 3.9107911 0.0000000 1.1046509 8.6150010 0.05429583 0.00000000 0.11716667 0.11716667 1.067377e-05 1.776251e-08 FALSE FALSE
ENST00000554081 ENSG00000185246 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF39 protein_coding nonsense_mediated_decay 20.3731 4.667238 33.14339 0.4358627 0.5267264 2.825428 0.2703865 0.4768947 0.0000000 0.2523103 0.0000000 -5.6055378 0.05905417 0.09340000 0.00000000 -0.09340000 2.791048e-02 1.776251e-08 FALSE TRUE
ENST00000554785 ENSG00000185246 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF39 protein_coding retained_intron 20.3731 4.667238 33.14339 0.4358627 0.5267264 2.825428 1.6833945 0.5129230 2.3578355 0.1292671 0.2268300 2.1788984 0.10270000 0.11716667 0.07113333 -0.04603333 5.886715e-01 1.776251e-08 FALSE TRUE
ENST00000556718 ENSG00000185246 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF39 protein_coding retained_intron 20.3731 4.667238 33.14339 0.4358627 0.5267264 2.825428 1.8470949 0.4687998 3.3794576 0.4687998 0.9844362 2.8235600 0.07180000 0.09160000 0.10276667 0.01116667 4.311978e-01 1.776251e-08 FALSE FALSE
MSTRG.9459.1 ENSG00000185246 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF39 protein_coding   20.3731 4.667238 33.14339 0.4358627 0.5267264 2.825428 2.5156143 0.0000000 3.8967130 0.0000000 0.7816201 8.6098115 0.12061250 0.00000000 0.11783333 0.11783333 7.043411e-08 1.776251e-08 TRUE TRUE
MSTRG.9459.7 ENSG00000185246 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF39 protein_coding   20.3731 4.667238 33.14339 0.4358627 0.5267264 2.825428 0.3194730 0.6620925 0.4795721 0.3328009 0.1579774 -0.4571385 0.02657917 0.15096667 0.01433333 -0.13663333 3.878218e-01 1.776251e-08 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185246 E001 0.4439371 0.0215539909 1.000000e+00 1.000000e+00 14 45084076 45084088 13 + 0.135 0.000 -9.748
ENSG00000185246 E002 1.6158141 0.0159700351 1.622074e-01 2.779786e-01 14 45084089 45084106 18 + 0.369 0.000 -11.605
ENSG00000185246 E003 1.6158141 0.0159700351 1.622074e-01 2.779786e-01 14 45084107 45084115 9 + 0.369 0.000 -11.605
ENSG00000185246 E004 1.6158141 0.0159700351 1.622074e-01 2.779786e-01 14 45084116 45084117 2 + 0.369 0.000 -11.605
ENSG00000185246 E005 4.5740487 0.0043707940 5.978109e-03 1.854972e-02 14 45084118 45084127 10 + 0.679 0.000 -13.100
ENSG00000185246 E006 65.0852840 0.0003563435 4.946090e-04 2.139369e-03 14 45084128 45084249 122 + 1.694 1.486 -0.711
ENSG00000185246 E007 57.6505776 0.0011044691 6.527154e-03 1.999304e-02 14 45095221 45095238 18 + 1.638 1.473 -0.564
ENSG00000185246 E008 77.7913395 0.0022059591 3.756098e-05 2.179690e-04 14 45095239 45095305 67 + 1.773 1.522 -0.854
ENSG00000185246 E009 38.8406018 0.0014772492 1.432219e-04 7.175185e-04 14 45095306 45095308 3 + 1.485 1.148 -1.178
ENSG00000185246 E010 82.6600006 0.0003704251 5.463076e-06 3.855330e-05 14 45095309 45095405 97 + 1.800 1.543 -0.870
ENSG00000185246 E011 72.8019478 0.0003417229 4.125222e-04 1.822341e-03 14 45095406 45095445 40 + 1.741 1.543 -0.671
ENSG00000185246 E012 87.3340367 0.0003424840 1.708225e-03 6.307483e-03 14 45095446 45095533 88 + 1.814 1.665 -0.503
ENSG00000185246 E013 47.0495333 0.0005001149 1.268271e-01 2.297301e-01 14 45095534 45095563 30 + 1.542 1.460 -0.280
ENSG00000185246 E014 0.8438645 0.0143033365 1.499005e-02 4.046654e-02 14 45095564 45095733 170 + 0.095 0.558 3.424
ENSG00000185246 E015 82.5495900 0.0003571437 5.745364e-01 7.003285e-01 14 45096103 45096197 95 + 1.769 1.773 0.013
ENSG00000185246 E016 69.1760033 0.0006518933 1.272273e-01 2.302722e-01 14 45096198 45096228 31 + 1.701 1.640 -0.208
ENSG00000185246 E017 34.9719862 0.0267827383 7.347789e-02 1.490908e-01 14 45096229 45096423 195 + 1.427 1.248 -0.624
ENSG00000185246 E018 28.2704663 0.0057990090 1.639051e-01 2.802390e-01 14 45096424 45096492 69 + 1.334 1.222 -0.393
ENSG00000185246 E019 38.5482347 0.0005347235 5.077923e-05 2.856965e-04 14 45096493 45096595 103 + 1.484 1.120 -1.273
ENSG00000185246 E020 40.9206578 0.0011221578 9.426272e-05 4.950459e-04 14 45096596 45096648 53 + 1.509 1.174 -1.168
ENSG00000185246 E021 51.8399281 0.0004638432 8.989746e-04 3.610516e-03 14 45096649 45096758 110 + 1.600 1.373 -0.779
ENSG00000185246 E022 26.5093944 0.0007534978 2.975047e-03 1.018881e-02 14 45096759 45096886 128 + 1.324 1.024 -1.068
ENSG00000185246 E023 57.3628863 0.0003834179 4.321836e-02 9.695190e-02 14 45096887 45096905 19 + 1.628 1.521 -0.366
ENSG00000185246 E024 88.3112371 0.0003401332 1.190897e-01 2.186403e-01 14 45096906 45097005 100 + 1.805 1.754 -0.173
ENSG00000185246 E025 113.4501798 0.0013647290 2.032618e-01 3.303055e-01 14 45102529 45102696 168 + 1.910 1.880 -0.098
ENSG00000185246 E026 135.7177349 0.0002779555 2.276667e-02 5.726186e-02 14 45107451 45107616 166 + 1.990 1.923 -0.226
ENSG00000185246 E027 133.9411387 0.0002876479 2.545724e-03 8.899837e-03 14 45108415 45108522 108 + 1.990 1.885 -0.351
ENSG00000185246 E028 125.1243806 0.0003890818 5.429960e-01 6.736847e-01 14 45109616 45109731 116 + 1.946 1.953 0.026
ENSG00000185246 E029 61.7427313 0.0003793121 4.632763e-01 6.030947e-01 14 45109732 45109742 11 + 1.632 1.712 0.270
ENSG00000185246 E030 81.7072305 0.0003361837 7.094913e-01 8.068614e-01 14 45109743 45109780 38 + 1.755 1.810 0.186
ENSG00000185246 E031 21.3818982 0.0010275030 9.150406e-01 9.502098e-01 14 45109781 45109893 113 + 1.200 1.244 0.155
ENSG00000185246 E032 58.8213998 0.0004257406 5.103793e-01 6.456017e-01 14 45109894 45109993 100 + 1.613 1.689 0.258
ENSG00000185246 E033 24.8774731 0.0037001022 1.160441e-01 2.143044e-01 14 45109994 45110070 77 + 1.285 1.148 -0.484
ENSG00000185246 E034 18.4206081 0.0009920277 3.999957e-01 5.437181e-01 14 45110071 45110093 23 + 1.154 1.090 -0.230
ENSG00000185246 E035 144.0074105 0.0004930587 9.965615e-01 1.000000e+00 14 45110094 45110220 127 + 2.002 2.037 0.117
ENSG00000185246 E036 109.0600504 0.0002753742 1.140550e-01 2.114286e-01 14 45110549 45110602 54 + 1.871 1.978 0.360
ENSG00000185246 E037 86.2046702 0.0004522665 8.075777e-02 1.607291e-01 14 45110603 45110628 26 + 1.766 1.890 0.419
ENSG00000185246 E038 201.2547110 0.0007101176 8.916214e-01 9.347814e-01 14 45110629 45110817 189 + 2.146 2.186 0.134
ENSG00000185246 E039 210.6436883 0.0010022614 2.148659e-01 3.442867e-01 14 45112318 45112502 185 + 2.160 2.239 0.264
ENSG00000185246 E040 120.9056794 0.0003117276 1.543752e-04 7.665835e-04 14 45114183 45114257 75 + 1.900 2.092 0.645
ENSG00000185246 E041 164.3285445 0.0002377775 1.465412e-03 5.524403e-03 14 45114494 45114614 121 + 2.040 2.191 0.504
ENSG00000185246 E042 9.8332534 0.0017641032 2.353198e-02 5.884609e-02 14 45114615 45114856 242 + 0.835 1.148 1.160
ENSG00000185246 E043 282.1831558 0.0097028275 2.025412e-15 7.346511e-14 14 45114857 45116282 1426 + 2.205 2.634 1.433