ENSG00000185236

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000328024 ENSG00000185236 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB11B protein_coding protein_coding 55.37635 91.56031 38.25585 5.799439 0.8273546 -1.258823 48.89429 85.737407 32.575028 4.423343 1.0914035 -1.39588383 0.8592667 0.9378667 0.8513667 -0.0865 0.01274889 0.01066466 FALSE  
ENST00000594216 ENSG00000185236 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB11B protein_coding protein_coding 55.37635 91.56031 38.25585 5.799439 0.8273546 -1.258823 6.07421 5.503737 5.622658 1.297602 0.7589792 0.03078545 0.1311417 0.0587000 0.1471000 0.0884 0.01066466 0.01066466 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185236 E001 0.1817044 3.989840e-02 1.000000e+00   19 8389981 8390056 76 + 0.001 0.081 7.031
ENSG00000185236 E002 29.2532849 9.443613e-03 6.516330e-01 7.624601e-01 19 8390360 8390362 3 + 1.422 1.406 -0.056
ENSG00000185236 E003 143.4239140 2.686106e-04 2.177731e-04 1.039939e-03 19 8390363 8390388 26 + 2.163 2.063 -0.333
ENSG00000185236 E004 188.1056950 3.815523e-04 1.184905e-03 4.589775e-03 19 8390389 8390392 4 + 2.260 2.186 -0.247
ENSG00000185236 E005 432.0415243 5.102006e-04 6.962717e-02 1.427391e-01 19 8390393 8390456 64 + 2.578 2.563 -0.053
ENSG00000185236 E006 850.4856957 2.046279e-03 2.257848e-01 3.573187e-01 19 8399863 8400058 196 + 2.864 2.863 -0.005
ENSG00000185236 E007 2.5411559 6.013351e-03 6.202857e-01 7.375037e-01 19 8400059 8400342 284 + 0.408 0.511 0.526
ENSG00000185236 E008 833.1199244 9.146139e-05 4.685864e-01 6.079919e-01 19 8402086 8402279 194 + 2.842 2.854 0.039
ENSG00000185236 E009 552.1143684 2.473605e-04 8.872529e-02 1.731771e-01 19 8402485 8402565 81 + 2.679 2.671 -0.026
ENSG00000185236 E010 61.4783190 6.025299e-03 5.111425e-07 4.513373e-06 19 8402566 8402924 359 + 1.911 1.639 -0.921
ENSG00000185236 E011 1490.2246437 2.178402e-03 2.478925e-06 1.890143e-05 19 8403413 8404434 1022 + 3.020 3.132 0.372