ENSG00000185163

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000329073 ENSG00000185163 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX51 protein_coding retained_intron 38.98385 54.96632 31.07176 2.692492 0.871579 -0.8227421 2.393240 2.1536134 2.306401 0.16732023 0.1097081 0.09844205 0.06590417 0.039066667 0.07433333 0.03526667 1.759523e-04 1.089884e-11 FALSE FALSE
ENST00000397333 ENSG00000185163 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX51 protein_coding protein_coding 38.98385 54.96632 31.07176 2.692492 0.871579 -0.8227421 11.135564 14.0788387 9.440019 1.55565397 0.6524129 -0.57616359 0.29019167 0.255366667 0.30516667 0.04980000 5.580064e-01 1.089884e-11 FALSE TRUE
ENST00000462829 ENSG00000185163 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX51 protein_coding retained_intron 38.98385 54.96632 31.07176 2.692492 0.871579 -0.8227421 1.717806 0.4133131 1.307495 0.21809752 0.4402919 1.63800011 0.05045000 0.007233333 0.04253333 0.03530000 3.015179e-02 1.089884e-11   FALSE
ENST00000541489 ENSG00000185163 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX51 protein_coding retained_intron 38.98385 54.96632 31.07176 2.692492 0.871579 -0.8227421 10.466539 20.0471337 5.942815 0.56828627 0.2697499 -1.75247154 0.25332917 0.365933333 0.19106667 -0.17486667 1.089884e-11 1.089884e-11 FALSE TRUE
ENST00000545991 ENSG00000185163 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX51 protein_coding retained_intron 38.98385 54.96632 31.07176 2.692492 0.871579 -0.8227421 2.777054 1.3156708 2.658897 0.08731759 0.2309279 1.00952115 0.07935000 0.024066667 0.08536667 0.06130000 2.244969e-11 1.089884e-11 FALSE FALSE
ENST00000546058 ENSG00000185163 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX51 protein_coding retained_intron 38.98385 54.96632 31.07176 2.692492 0.871579 -0.8227421 3.249531 2.5674451 3.139903 0.74175941 0.2366015 0.28936561 0.08877500 0.045666667 0.10093333 0.05526667 2.164425e-02 1.089884e-11 TRUE FALSE
MSTRG.8340.4 ENSG00000185163 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX51 protein_coding   38.98385 54.96632 31.07176 2.692492 0.871579 -0.8227421 2.179195 3.9727085 2.958168 0.36938965 0.2122308 -0.42417734 0.05478750 0.072400000 0.09500000 0.02260000 2.345480e-01 1.089884e-11 TRUE TRUE
MSTRG.8340.7 ENSG00000185163 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX51 protein_coding   38.98385 54.96632 31.07176 2.692492 0.871579 -0.8227421 2.834370 6.0013700 1.998693 0.44330037 0.2431035 -1.58143665 0.06871250 0.109600000 0.06400000 -0.04560000 1.535248e-02 1.089884e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185163 E001 0.000000       12 132135329 132135424 96 -      
ENSG00000185163 E002 0.000000       12 132135566 132135635 70 -      
ENSG00000185163 E003 0.000000       12 132135949 132135983 35 -      
ENSG00000185163 E004 0.000000       12 132136232 132136369 138 -      
ENSG00000185163 E005 0.000000       12 132136370 132136593 224 -      
ENSG00000185163 E006 30.558304 0.0026927363 4.352573e-01 5.774909e-01 12 132136594 132136691 98 - 1.419 1.465 0.159
ENSG00000185163 E007 234.922788 0.0042530593 3.479265e-03 1.166468e-02 12 132136692 132137320 629 - 2.410 2.289 -0.403
ENSG00000185163 E008 106.474498 0.0044942362 3.742111e-06 2.742229e-05 12 132137321 132137434 114 - 2.135 1.908 -0.761
ENSG00000185163 E009 486.216691 0.0005936947 1.024140e-13 2.908849e-12 12 132137435 132138400 966 - 2.747 2.592 -0.516
ENSG00000185163 E010 125.970663 0.0002480194 7.517648e-02 1.518189e-01 12 132138401 132138579 179 - 2.107 2.036 -0.238
ENSG00000185163 E011 104.997912 0.0003179611 2.776430e-01 4.172474e-01 12 132138580 132138707 128 - 2.016 1.965 -0.172
ENSG00000185163 E012 211.987634 0.0046685345 5.097479e-01 6.450310e-01 12 132138708 132138872 165 - 2.309 2.272 -0.124
ENSG00000185163 E013 470.534263 0.0003250827 2.778051e-02 6.757921e-02 12 132138873 132139112 240 - 2.605 2.636 0.105
ENSG00000185163 E014 513.071471 0.0011011331 9.326533e-06 6.241861e-05 12 132139113 132139298 186 - 2.599 2.691 0.306
ENSG00000185163 E015 354.735764 0.0001458699 7.485687e-08 7.789843e-07 12 132139635 132139769 135 - 2.428 2.531 0.343
ENSG00000185163 E016 10.044298 0.0476220581 1.531049e-02 4.119072e-02 12 132139770 132139854 85 - 1.229 0.865 -1.331
ENSG00000185163 E017 4.931298 0.0177208110 3.250855e-03 1.100387e-02 12 132139855 132139860 6 - 0.995 0.570 -1.709
ENSG00000185163 E018 332.900316 0.0001445394 4.986880e-06 3.550222e-05 12 132139861 132139924 64 - 2.412 2.500 0.293
ENSG00000185163 E019 3.072810 0.0608922552 3.510517e-02 8.184510e-02 12 132139925 132139973 49 - 0.820 0.445 -1.649
ENSG00000185163 E020 389.338822 0.0001774787 4.680856e-04 2.038093e-03 12 132140098 132140199 102 - 2.503 2.562 0.197
ENSG00000185163 E021 1.369387 0.0097484668 6.921684e-03 2.101996e-02 12 132140422 132140422 1 - 0.630 0.162 -2.849
ENSG00000185163 E022 412.781677 0.0006252674 3.212170e-04 1.465822e-03 12 132140423 132140539 117 - 2.521 2.590 0.230
ENSG00000185163 E023 340.181573 0.0001678217 1.005631e-02 2.889902e-02 12 132140620 132140735 116 - 2.455 2.498 0.145
ENSG00000185163 E024 2.050108 0.0750414924 1.826423e-01 3.045092e-01 12 132140736 132140821 86 - 0.630 0.372 -1.270
ENSG00000185163 E025 386.422878 0.0016559159 8.123479e-05 4.338300e-04 12 132140831 132141005 175 - 2.471 2.569 0.328
ENSG00000185163 E026 163.020631 0.0002354019 3.590595e-05 2.092943e-04 12 132141006 132141020 15 - 2.078 2.197 0.398
ENSG00000185163 E027 18.807269 0.0011082936 5.137354e-06 3.646520e-05 12 132141021 132141102 82 - 1.483 1.115 -1.292
ENSG00000185163 E028 41.991303 0.0008556428 1.014333e-11 2.109820e-10 12 132141103 132141274 172 - 1.822 1.437 -1.313
ENSG00000185163 E029 347.877643 0.0002126547 1.548381e-06 1.233749e-05 12 132141275 132141420 146 - 2.427 2.520 0.310
ENSG00000185163 E030 299.969369 0.0001661182 3.543693e-03 1.185091e-02 12 132141498 132141606 109 - 2.391 2.446 0.183
ENSG00000185163 E031 56.116266 0.0059510280 1.028412e-14 3.377181e-13 12 132141607 132141849 243 - 1.984 1.513 -1.592
ENSG00000185163 E032 303.078919 0.0002113337 1.305062e-02 3.603075e-02 12 132141850 132141956 107 - 2.404 2.450 0.151
ENSG00000185163 E033 59.292997 0.0054502464 1.351161e-07 1.338563e-06 12 132141957 132142118 162 - 1.939 1.626 -1.057
ENSG00000185163 E034 262.172787 0.0001636691 7.705008e-01 8.518070e-01 12 132142119 132142190 72 - 2.376 2.372 -0.015
ENSG00000185163 E035 220.956353 0.0003568997 1.885753e-01 3.119464e-01 12 132142277 132142317 41 - 2.280 2.304 0.081
ENSG00000185163 E036 280.829748 0.0002888167 9.992652e-01 1.000000e+00 12 132142318 132142422 105 - 2.410 2.399 -0.038
ENSG00000185163 E037 275.533795 0.0019352848 9.983983e-01 1.000000e+00 12 132142728 132142878 151 - 2.400 2.391 -0.031
ENSG00000185163 E038 49.820241 0.0195321965 3.335371e-12 7.515177e-11 12 132142879 132143639 761 - 1.993 1.391 -2.046
ENSG00000185163 E039 169.121995 0.0042660416 3.305325e-04 1.502901e-03 12 132143695 132143909 215 - 2.290 2.131 -0.531
ENSG00000185163 E040 91.731617 0.0058980985 9.980064e-27 1.254607e-24 12 132143993 132144319 327 - 2.241 1.670 -1.922