ENSG00000185088

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000411926 ENSG00000185088 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS27L protein_coding protein_coding 45.31288 51.19364 53.54014 10.96157 2.302026 0.06464372 31.16683 36.53402 33.71462 7.894867 1.2455753 -0.1158332 0.6855875 0.7131000 0.6302333 -0.08286667 0.001794905 0.001794905 FALSE  
ENST00000439025 ENSG00000185088 HEK293_OSMI2_2hA HEK293_TMG_2hB RPS27L protein_coding protein_coding 45.31288 51.19364 53.54014 10.96157 2.302026 0.06464372 10.97034 11.55852 14.83097 2.256314 0.2975923 0.3593812 0.2453250 0.2274333 0.2776000 0.05016667 0.033519479 0.001794905    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185088 E001 0.0000000       15 63125872 63126020 149 -      
ENSG00000185088 E002 26.0315132 0.0007599827 0.0005402770 0.002310888 15 63148249 63153852 5604 - 1.555 1.328 -0.784
ENSG00000185088 E003 21.2061196 0.0009380816 0.0647901034 0.134701726 15 63153853 63153858 6 - 1.422 1.288 -0.466
ENSG00000185088 E004 328.7859227 0.0002024109 0.0003014982 0.001385651 15 63153859 63154060 202 - 2.486 2.553 0.221
ENSG00000185088 E005 8.1206677 0.0076939104 0.4919743617 0.629193531 15 63154061 63154970 910 - 0.910 0.989 0.296
ENSG00000185088 E006 4.1810972 0.0037114916 0.1188066289 0.218242424 15 63154971 63155620 650 - 0.830 0.615 -0.885
ENSG00000185088 E007 519.5010804 0.0001337943 0.7847783503 0.862002633 15 63155621 63155731 111 - 2.721 2.725 0.013
ENSG00000185088 E008 504.5981763 0.0001402390 0.5220928367 0.655959954 15 63156413 63156521 109 - 2.715 2.706 -0.028
ENSG00000185088 E009 1.1145414 0.2748320360 0.7522176243 0.838484920 15 63156522 63156786 265 - 0.306 0.347 0.261
ENSG00000185088 E010 3.3641228 0.0180289941 0.5868898335 0.710329789 15 63156787 63156973 187 - 0.608 0.701 0.398
ENSG00000185088 E011 4.6437089 0.0045230474 0.1928136476 0.317300641 15 63156974 63157399 426 - 0.851 0.676 -0.705
ENSG00000185088 E012 294.8576436 0.0002280572 0.1433743269 0.252607147 15 63157400 63157499 100 - 2.493 2.464 -0.096
ENSG00000185088 E013 0.9900693 0.1846163209 0.5072263112 0.642810814 15 63157916 63158021 106 - 0.372 0.213 -1.095