ENSG00000185049

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000382882 ENSG00000185049 HEK293_OSMI2_2hA HEK293_TMG_2hB NELFA protein_coding protein_coding 40.34841 57.87612 27.6722 2.428912 1.275313 -1.064259 21.201133 29.831903 20.93221 5.992489 0.4453866 -0.5109256 0.5521958 0.5154000 0.75826667 0.2428667 1.076157e-01 8.846354e-08 FALSE TRUE
ENST00000467661 ENSG00000185049 HEK293_OSMI2_2hA HEK293_TMG_2hB NELFA protein_coding retained_intron 40.34841 57.87612 27.6722 2.428912 1.275313 -1.064259 4.923521 8.365559 1.45010 2.790026 0.5768921 -2.5201187 0.1139250 0.1490667 0.05226667 -0.0968000 2.049178e-01 8.846354e-08 FALSE TRUE
ENST00000542778 ENSG00000185049 HEK293_OSMI2_2hA HEK293_TMG_2hB NELFA protein_coding protein_coding 40.34841 57.87612 27.6722 2.428912 1.275313 -1.064259 8.085628 12.314154 0.00000 5.924631 0.0000000 -10.2672729 0.1756750 0.2091667 0.00000000 -0.2091667 8.846354e-08 8.846354e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185049 E001 1.6887731 0.0080056125 9.374750e-01 9.646664e-01 4 1982717 1982722 6 - 0.403 0.390 -0.073
ENSG00000185049 E002 3.4172976 0.0045712887 5.466417e-02 1.174106e-01 4 1982723 1982729 7 - 0.305 0.656 1.793
ENSG00000185049 E003 59.6482471 0.0004383118 6.907308e-01 7.923559e-01 4 1982730 1982781 52 - 1.703 1.726 0.077
ENSG00000185049 E004 359.0808502 0.0001885724 3.869699e-13 1.012293e-11 4 1982782 1983049 268 - 2.361 2.528 0.558
ENSG00000185049 E005 851.8188860 0.0024374122 4.147132e-03 1.355524e-02 4 1983050 1983503 454 - 2.810 2.887 0.258
ENSG00000185049 E006 411.5859158 0.0066054891 7.989734e-01 8.717316e-01 4 1983596 1983695 100 - 2.558 2.553 -0.017
ENSG00000185049 E007 571.5095938 0.0013217471 9.432424e-01 9.684011e-01 4 1983848 1984113 266 - 2.692 2.696 0.012
ENSG00000185049 E008 335.2884079 0.0002279413 5.848061e-01 7.086348e-01 4 1984808 1984919 112 - 2.467 2.457 -0.032
ENSG00000185049 E009 313.7207726 0.0008591174 6.962976e-01 7.966873e-01 4 1985776 1985864 89 - 2.423 2.437 0.046
ENSG00000185049 E010 167.7788713 0.0012288700 9.376225e-01 9.647762e-01 4 1986114 1986114 1 - 2.165 2.165 0.001
ENSG00000185049 E011 307.9482862 0.0001452642 8.004114e-01 8.727828e-01 4 1986115 1986183 69 - 2.427 2.423 -0.011
ENSG00000185049 E012 193.0300896 0.0002293987 4.677380e-01 6.072345e-01 4 1986272 1986279 8 - 2.235 2.217 -0.062
ENSG00000185049 E013 331.9636528 0.0015887642 1.634088e-01 2.796049e-01 4 1986280 1986381 102 - 2.482 2.448 -0.113
ENSG00000185049 E014 166.1020307 0.0013807984 6.465240e-01 7.583980e-01 4 1986382 1986383 2 - 2.165 2.153 -0.041
ENSG00000185049 E015 185.2244562 0.0010872691 5.374247e-01 6.689939e-01 4 1986384 1986402 19 - 2.215 2.199 -0.055
ENSG00000185049 E016 7.2062036 0.0485965503 4.119840e-01 5.555375e-01 4 1986403 1986560 158 - 0.961 0.830 -0.500
ENSG00000185049 E017 14.1491604 0.0013007662 1.082651e-03 4.244809e-03 4 1987501 1987917 417 - 1.320 1.024 -1.057
ENSG00000185049 E018 172.2397498 0.0047849867 5.745360e-01 7.003285e-01 4 1987918 1987941 24 - 2.186 2.168 -0.059
ENSG00000185049 E019 221.6240934 0.0014514942 2.809129e-01 4.207730e-01 4 1987942 1988007 66 - 2.303 2.273 -0.099
ENSG00000185049 E020 320.8039520 0.0002193354 4.544546e-03 1.466571e-02 4 1989708 1989869 162 - 2.485 2.424 -0.201
ENSG00000185049 E021 2.7832553 0.0504967040 3.308141e-01 4.743834e-01 4 1989870 1990054 185 - 0.659 0.488 -0.781
ENSG00000185049 E022 0.2966881 0.0272583374 4.980116e-02   4 1990418 1990501 84 - 0.305 0.000 -10.773
ENSG00000185049 E023 220.6560540 0.0010179945 7.827285e-03 2.334728e-02 4 1991544 1991587 44 - 2.335 2.259 -0.251
ENSG00000185049 E024 345.1410638 0.0008212360 1.053580e-01 1.986421e-01 4 1991588 1991715 128 - 2.500 2.464 -0.119
ENSG00000185049 E025 3.5814905 0.0044187047 3.998739e-02 9.101219e-02 4 1991716 1991730 15 - 0.820 0.516 -1.296
ENSG00000185049 E026 4.7523036 0.0193067624 3.950198e-01 5.388642e-01 4 1991731 1992244 514 - 0.785 0.659 -0.519
ENSG00000185049 E027 3.2334308 0.0047775854 4.442053e-01 5.857672e-01 4 1992403 1992624 222 - 0.483 0.614 0.611
ENSG00000185049 E028 0.6287620 0.0181168372 3.791257e-01 5.234094e-01 4 2003838 2004150 313 - 0.305 0.151 -1.297
ENSG00000185049 E029 29.8535618 0.0007176167 2.183342e-02 5.533036e-02 4 2006998 2007199 202 - 1.287 1.463 0.612
ENSG00000185049 E030 2.6110987 0.0106786612 2.045020e-01 3.317943e-01 4 2007963 2007971 9 - 0.305 0.544 1.294
ENSG00000185049 E031 6.9173133 0.0102255715 1.545297e-01 2.677910e-01 4 2007972 2008046 75 - 0.660 0.874 0.860
ENSG00000185049 E032 364.3221936 0.0016673210 7.805122e-02 1.563426e-01 4 2008750 2009235 486 - 2.530 2.487 -0.145
ENSG00000185049 E033 1.3147814 0.2000734929 6.283529e-01 7.438997e-01 4 2017665 2017742 78 - 0.403 0.309 -0.559
ENSG00000185049 E034 1.9037895 0.1095595572 4.186368e-01 5.618729e-01 4 2023405 2023536 132 - 0.550 0.387 -0.824
ENSG00000185049 E035 7.6178940 0.0022469568 1.062066e-01 1.999337e-01 4 2041716 2041903 188 - 0.706 0.928 0.875