ENSG00000185033

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000332496 ENSG00000185033 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA4B protein_coding protein_coding 6.80397 8.426408 5.0417 0.4045511 0.2349694 -0.73986 1.2892721 2.1915659 0.8601609 0.9761203 0.55395899 -1.3391758 0.1873167 0.2716667 0.1699000 -0.10176667 7.928244e-01 3.900305e-12 FALSE TRUE
ENST00000411539 ENSG00000185033 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA4B protein_coding protein_coding 6.80397 8.426408 5.0417 0.4045511 0.2349694 -0.73986 3.5685134 3.9870768 2.4340654 1.6300646 0.70351264 -0.7096624 0.5259083 0.4593000 0.4858333 0.02653333 9.765740e-01 3.900305e-12 FALSE TRUE
ENST00000559247 ENSG00000185033 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA4B protein_coding protein_coding 6.80397 8.426408 5.0417 0.4045511 0.2349694 -0.73986 0.1800389 0.9093133 0.2264363 0.4592694 0.22643635 -1.9591048 0.0248375 0.1086667 0.0430000 -0.06566667 6.102304e-01 3.900305e-12 FALSE TRUE
ENST00000561085 ENSG00000185033 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA4B protein_coding protein_coding 6.80397 8.426408 5.0417 0.4045511 0.2349694 -0.73986 0.2186044 0.0000000 0.5116802 0.0000000 0.04749358 5.7050939 0.0370000 0.0000000 0.1023000 0.10230000 3.900305e-12 3.900305e-12   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000185033 E001 0.0000000       15 90160604 90160700 97 +      
ENSG00000185033 E002 0.2214452 0.0385673197 1.000000e+00   15 90184918 90184919 2 + 0.000 0.091 8.376
ENSG00000185033 E003 0.6696487 0.0276296537 9.280046e-02 0.1795050670 15 90184920 90184931 12 + 0.374 0.091 -2.550
ENSG00000185033 E004 3.0633823 0.0289956824 7.396934e-01 0.8293445710 15 90184932 90184962 31 + 0.622 0.577 -0.199
ENSG00000185033 E005 4.5673437 0.0123441712 5.086135e-01 0.6440034347 15 90184963 90185081 119 + 0.779 0.694 -0.342
ENSG00000185033 E006 0.0000000       15 90190344 90190482 139 +      
ENSG00000185033 E007 0.1482932 0.0424923653 1.000000e+00   15 90191922 90191927 6 + 0.000 0.091 10.503
ENSG00000185033 E008 0.3299976 0.0274424043 4.663953e-01   15 90191928 90192026 99 + 0.000 0.166 11.564
ENSG00000185033 E009 0.0000000       15 90193129 90193563 435 +      
ENSG00000185033 E010 4.2570011 0.1015126207 2.505938e-01 0.3866436883 15 90201330 90201420 91 + 0.810 0.612 -0.817
ENSG00000185033 E011 5.2009974 0.2414017383 6.392903e-01 0.7527020184 15 90201421 90201457 37 + 0.809 0.730 -0.318
ENSG00000185033 E012 9.7117607 0.0945879465 5.863915e-01 0.7099680008 15 90201458 90201535 78 + 1.023 0.979 -0.162
ENSG00000185033 E013 17.3468933 0.0244336024 4.672794e-02 0.1033394268 15 90201536 90201735 200 + 1.350 1.157 -0.677
ENSG00000185033 E014 0.0000000       15 90214937 90214964 28 +      
ENSG00000185033 E015 0.1482932 0.0424923653 1.000000e+00   15 90217056 90217438 383 + 0.000 0.091 10.503
ENSG00000185033 E016 31.6977389 0.0200504058 3.307829e-01 0.4743565675 15 90217439 90217602 164 + 1.523 1.455 -0.233
ENSG00000185033 E017 0.6986918 0.0172671820 7.306684e-01 0.8225359925 15 90217617 90217766 150 + 0.163 0.230 0.618
ENSG00000185033 E018 28.7178943 0.0140823370 9.844377e-02 0.1881336422 15 90217767 90217829 63 + 1.517 1.394 -0.421
ENSG00000185033 E019 0.4439371 0.0215742449 1.998605e-02 0.0514561301 15 90217830 90217928 99 + 0.374 0.000 -14.246
ENSG00000185033 E020 1.7650733 0.0778529383 8.150681e-01 0.8828470126 15 90219208 90219792 585 + 0.450 0.418 -0.173
ENSG00000185033 E021 38.0519217 0.0124556873 1.750092e-01 0.2946819866 15 90219793 90219891 99 + 1.609 1.523 -0.291
ENSG00000185033 E022 0.7718438 0.0295431796 7.308511e-01 0.8226537107 15 90219892 90220218 327 + 0.163 0.231 0.624
ENSG00000185033 E023 41.7284416 0.0061728530 1.094243e-01 0.2047155198 15 90220982 90221093 112 + 1.646 1.556 -0.305
ENSG00000185033 E024 42.2205219 0.0012054974 1.713643e-01 0.2899672742 15 90221367 90221480 114 + 1.632 1.567 -0.221
ENSG00000185033 E025 20.8440847 0.0021880101 2.037986e-01 0.3309648954 15 90221614 90221617 4 + 1.350 1.262 -0.309
ENSG00000185033 E026 39.2492936 0.0101543024 2.955333e-01 0.4366642762 15 90221618 90221749 132 + 1.598 1.539 -0.204
ENSG00000185033 E027 16.4895954 0.0013388909 1.004863e-01 0.1911922476 15 90221750 90221765 16 + 1.283 1.155 -0.455
ENSG00000185033 E028 40.1770244 0.0008065076 1.320621e-01 0.2369807602 15 90223559 90223740 182 + 1.618 1.544 -0.252
ENSG00000185033 E029 1.2553328 0.2331700669 7.938637e-01 0.8682438873 15 90223741 90223792 52 + 0.281 0.371 0.569
ENSG00000185033 E030 25.2455303 0.0007826010 1.924534e-01 0.3168482489 15 90223838 90223876 39 + 1.430 1.351 -0.276
ENSG00000185033 E031 33.3953539 0.0079036438 6.085755e-02 0.1280831912 15 90223877 90223988 112 + 1.573 1.451 -0.418
ENSG00000185033 E032 24.2291911 0.0105877468 5.629987e-01 0.6905379082 15 90224968 90225001 34 + 1.376 1.342 -0.116
ENSG00000185033 E033 28.1792131 0.0007698788 3.571311e-01 0.5015487550 15 90225002 90225071 70 + 1.452 1.402 -0.173
ENSG00000185033 E034 30.7359729 0.0007139657 9.178514e-01 0.9520833614 15 90225072 90225178 107 + 1.452 1.458 0.021
ENSG00000185033 E035 0.2924217 0.0272910412 7.001426e-02   15 90225179 90225281 103 + 0.281 0.000 -13.634
ENSG00000185033 E036 22.5847888 0.0013998730 4.900237e-01 0.6273777397 15 90225282 90225323 42 + 1.283 1.351 0.235
ENSG00000185033 E037 24.6937546 0.0010165804 8.709273e-01 0.9210215868 15 90225324 90225397 74 + 1.367 1.368 0.003
ENSG00000185033 E038 1.5573195 0.0530537527 3.009389e-01 0.4427151379 15 90225398 90225427 30 + 0.515 0.333 -0.980
ENSG00000185033 E039 1.5468252 0.0091320969 1.890550e-02 0.0491260103 15 90225428 90225608 181 + 0.622 0.230 -2.190
ENSG00000185033 E040 47.0051590 0.0004599110 3.594200e-01 0.5037511306 15 90225661 90225827 167 + 1.663 1.628 -0.121
ENSG00000185033 E041 2.1734807 0.0527757855 5.683194e-01 0.6950120884 15 90227239 90227537 299 + 0.374 0.493 0.631
ENSG00000185033 E042 0.7384326 0.0283842613 7.309491e-01 0.8227052792 15 90227538 90227556 19 + 0.163 0.230 0.621
ENSG00000185033 E043 46.9938161 0.0005354997 6.554374e-01 0.7653199017 15 90227557 90227642 86 + 1.613 1.651 0.128
ENSG00000185033 E044 94.1238985 0.0203155466 2.124294e-01 0.3413989102 15 90227904 90228264 361 + 1.864 1.967 0.345
ENSG00000185033 E045 226.7365596 0.0018887879 3.573309e-05 0.0002084085 15 90228265 90229253 989 + 2.217 2.353 0.455
ENSG00000185033 E046 99.7387393 0.0149373936 4.443380e-03 0.0143813744 15 90229254 90229679 426 + 1.808 2.018 0.707