ENSG00000184481

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000341558 ENSG00000184481 HEK293_OSMI2_2hA HEK293_TMG_2hB FOXO4 protein_coding protein_coding 27.14955 53.26486 15.19946 0.7713899 0.1440634 -1.808486 3.8691713 12.4477340 0.00000 2.2863867 0.0000000 -10.282826 0.08897917 0.23396667 0.0000000 -0.2339667 2.752358e-18 2.752358e-18 FALSE TRUE
ENST00000374259 ENSG00000184481 HEK293_OSMI2_2hA HEK293_TMG_2hB FOXO4 protein_coding protein_coding 27.14955 53.26486 15.19946 0.7713899 0.1440634 -1.808486 22.2056770 39.9385526 13.11875 2.1250311 0.6174168 -1.605413 0.85230417 0.74966667 0.8632333 0.1135667 2.243075e-01 2.752358e-18 FALSE TRUE
ENST00000466874 ENSG00000184481 HEK293_OSMI2_2hA HEK293_TMG_2hB FOXO4 protein_coding processed_transcript 27.14955 53.26486 15.19946 0.7713899 0.1440634 -1.808486 0.7480887 0.7480958 1.91632 0.3481554 0.5895121 1.345396 0.03990000 0.01396667 0.1259000 0.1119333 2.288368e-02 2.752358e-18 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000184481 E001 147.590189 0.0005244010 6.999231e-06 4.821056e-05 X 71095851 71096252 402 + 2.151 2.040 -0.371
ENSG00000184481 E002 473.791582 0.0007649443 7.124894e-06 4.896575e-05 X 71096253 71096528 276 + 2.611 2.559 -0.171
ENSG00000184481 E003 357.066457 0.0009169915 2.019807e-02 5.189975e-02 X 71096529 71096699 171 + 2.457 2.445 -0.041
ENSG00000184481 E004 249.035794 0.0003955819 3.983523e-02 9.072147e-02 X 71096700 71096860 161 + 2.299 2.289 -0.035
ENSG00000184481 E005 109.745795 0.0005055495 7.924965e-01 8.673272e-01 X 71096861 71096864 4 + 1.909 1.945 0.122
ENSG00000184481 E006 245.015470 0.0002460770 6.854549e-02 1.409563e-01 X 71096865 71096981 117 + 2.287 2.284 -0.011
ENSG00000184481 E007 1.798559 0.0523578462 1.858599e-01 3.085833e-01 X 71099308 71099453 146 + 0.537 0.312 -1.216
ENSG00000184481 E008 1252.798123 0.0004746054 3.472301e-05 2.032055e-04 X 71100684 71101740 1057 + 2.996 2.991 -0.018
ENSG00000184481 E009 1657.520581 0.0043308049 3.691552e-08 4.082862e-07 X 71102077 71103532 1456 + 2.962 3.143 0.604