ENSG00000184220

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000421999 ENSG00000184220 HEK293_OSMI2_2hA HEK293_TMG_2hB CMSS1 protein_coding protein_coding 65.78046 43.92114 105.5302 4.899151 2.745311 1.264477 11.44631 9.479574 16.16256 1.099501 0.4358858 0.7691333 0.1830042 0.21560000 0.1532333 -0.06236667 1.223301e-05 1.223301e-05 FALSE  
ENST00000463526 ENSG00000184220 HEK293_OSMI2_2hA HEK293_TMG_2hB CMSS1 protein_coding protein_coding 65.78046 43.92114 105.5302 4.899151 2.745311 1.264477 11.98747 4.032304 23.75420 2.070063 1.8745785 2.5555401 0.1575875 0.08526667 0.2247667 0.13950000 5.618891e-01 1.223301e-05    
ENST00000491299 ENSG00000184220 HEK293_OSMI2_2hA HEK293_TMG_2hB CMSS1 protein_coding nonsense_mediated_decay 65.78046 43.92114 105.5302 4.899151 2.745311 1.264477 39.52068 28.656386 61.09750 2.184351 1.9610119 1.0919893 0.6154667 0.65846667 0.5790667 -0.07940000 3.260694e-01 1.223301e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000184220 E001 0.1515154 0.0439069853 8.292088e-01   3 99817620 99817706 87 + 0.096 0.000 -9.970
ENSG00000184220 E002 0.0000000       3 99817707 99817810 104 +      
ENSG00000184220 E003 2.4390546 0.0061614655 4.344697e-03 1.410874e-02 3 99817811 99817835 25 + 0.672 0.155 -3.106
ENSG00000184220 E004 6.2494710 0.0025673756 1.705490e-03 6.299965e-03 3 99817836 99817836 1 + 0.984 0.553 -1.744
ENSG00000184220 E005 10.0847621 0.0017962956 3.603276e-02 8.364638e-02 3 99817837 99817839 3 + 1.117 0.896 -0.815
ENSG00000184220 E006 48.9033367 0.0004652381 3.980297e-04 1.766682e-03 3 99817840 99817861 22 + 1.761 1.584 -0.603
ENSG00000184220 E007 173.2116029 0.0005543018 4.660056e-06 3.340777e-05 3 99817862 99817882 21 + 2.285 2.158 -0.426
ENSG00000184220 E008 320.5021592 0.0001370851 4.064366e-12 9.015127e-11 3 99817883 99817911 29 + 2.553 2.418 -0.453
ENSG00000184220 E009 662.3099434 0.0002644915 1.679954e-12 3.966378e-11 3 99817912 99818043 132 + 2.856 2.754 -0.338
ENSG00000184220 E010 23.5955380 0.0060447512 1.048046e-01 1.978113e-01 3 100010131 100010180 50 + 1.434 1.307 -0.438
ENSG00000184220 E011 1.4716830 0.1659515929 6.611873e-01 7.696936e-01 3 100011703 100011758 56 + 0.348 0.435 0.487
ENSG00000184220 E012 0.0000000       3 100023319 100023522 204 +      
ENSG00000184220 E013 0.3289534 0.0325814282 6.926078e-01   3 100025428 100025558 131 + 0.096 0.155 0.801
ENSG00000184220 E014 0.3686942 0.0336105479 6.918853e-01 7.932581e-01 3 100039775 100041396 1622 + 0.096 0.155 0.804
ENSG00000184220 E015 0.2965864 0.0618277532 9.973561e-02   3 100051232 100051306 75 + 0.000 0.268 12.278
ENSG00000184220 E016 0.2966881 0.0272925531 3.987736e-01   3 100114930 100114998 69 + 0.174 0.000 -10.963
ENSG00000184220 E017 9.8687038 0.0054859399 1.280957e-03 4.913412e-03 3 100141530 100141633 104 + 1.141 0.758 -1.438
ENSG00000184220 E018 1.3725078 0.0097982329 8.182158e-01 8.851106e-01 3 100141634 100141645 12 + 0.394 0.359 -0.201
ENSG00000184220 E019 358.3973535 0.0001414186 4.496593e-05 2.561849e-04 3 100146973 100146975 3 + 2.579 2.508 -0.236
ENSG00000184220 E020 665.5394698 0.0001716614 8.960566e-06 6.023961e-05 3 100146976 100147061 86 + 2.842 2.785 -0.189
ENSG00000184220 E021 0.0000000       3 100159818 100159916 99 +      
ENSG00000184220 E022 558.1185846 0.0007574520 4.814109e-03 1.541379e-02 3 100160430 100160501 72 + 2.762 2.714 -0.159
ENSG00000184220 E023 265.6220829 0.0031936863 9.922013e-01 9.992269e-01 3 100162303 100162305 3 + 2.419 2.419 0.002
ENSG00000184220 E024 424.4254280 0.0001333190 8.888431e-01 9.329063e-01 3 100162306 100162352 47 + 2.620 2.632 0.039
ENSG00000184220 E025 592.6303954 0.0001151788 3.895316e-01 5.336070e-01 3 100162353 100162432 80 + 2.761 2.783 0.073
ENSG00000184220 E026 0.7771569 0.0144804778 4.207261e-01 5.639180e-01 3 100166333 100166334 2 + 0.298 0.155 -1.200
ENSG00000184220 E027 527.2745476 0.0001189252 1.604057e-01 2.756791e-01 3 100166335 100166394 60 + 2.706 2.737 0.104
ENSG00000184220 E028 573.3060323 0.0003795838 2.295722e-03 8.136753e-03 3 100167738 100167840 103 + 2.730 2.789 0.196
ENSG00000184220 E029 483.3020084 0.0002227370 2.000371e-02 5.149601e-02 3 100171839 100171899 61 + 2.661 2.709 0.160
ENSG00000184220 E030 3.0042270 0.0070334967 3.336622e-01 4.774016e-01 3 100171900 100172315 416 + 0.649 0.498 -0.685
ENSG00000184220 E031 552.0903542 0.0001508829 6.672616e-07 5.755883e-06 3 100172316 100172403 88 + 2.702 2.788 0.288
ENSG00000184220 E032 0.4438354 0.0456141575 3.176701e-01 4.605620e-01 3 100172404 100172404 1 + 0.096 0.268 1.793
ENSG00000184220 E033 1.4466022 0.0100903181 7.714346e-02 1.549111e-01 3 100176238 100176326 89 + 0.241 0.553 1.798
ENSG00000184220 E034 558.7679077 0.0007294571 4.752856e-08 5.151406e-07 3 100176327 100176415 89 + 2.694 2.807 0.374
ENSG00000184220 E035 801.2816983 0.0016790654 1.014536e-06 8.404058e-06 3 100178305 100181732 3428 + 2.849 2.964 0.382