ENSG00000184178

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000388940 ENSG00000184178 HEK293_OSMI2_2hA HEK293_TMG_2hB SCFD2 protein_coding protein_coding 12.40235 15.57172 8.335103 1.304565 0.2397924 -0.9008524 0.5825075 1.219198 0.0000000 0.3880986 0.0000000 -6.9415731 0.04425417 0.08223333 0.00000000 -0.08223333 5.425894e-05 1.409493e-14 FALSE TRUE
ENST00000401642 ENSG00000184178 HEK293_OSMI2_2hA HEK293_TMG_2hB SCFD2 protein_coding protein_coding 12.40235 15.57172 8.335103 1.304565 0.2397924 -0.9008524 6.8862830 9.081354 5.6247001 0.9126465 0.7165124 -0.6901564 0.55765000 0.58140000 0.67156667 0.09016667 5.788264e-01 1.409493e-14 FALSE TRUE
MSTRG.24881.1 ENSG00000184178 HEK293_OSMI2_2hA HEK293_TMG_2hB SCFD2 protein_coding   12.40235 15.57172 8.335103 1.304565 0.2397924 -0.9008524 2.4487013 0.000000 2.3541374 0.0000000 0.5142515 7.8851701 0.22687917 0.00000000 0.28656667 0.28656667 1.409493e-14 1.409493e-14 TRUE TRUE
MSTRG.24881.3 ENSG00000184178 HEK293_OSMI2_2hA HEK293_TMG_2hB SCFD2 protein_coding   12.40235 15.57172 8.335103 1.304565 0.2397924 -0.9008524 2.4848580 5.271164 0.3562653 0.7178419 0.3562653 -3.8498950 0.17122917 0.33636667 0.04186667 -0.29450000 4.492591e-02 1.409493e-14 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000184178 E001 0.147249 0.0449188286 2.404419e-01   4 52872432 52872584 153 - 0.168 0.000 -10.075
ENSG00000184178 E002 148.436930 0.0027750517 8.331230e-01 8.954485e-01 4 52872982 52873787 806 - 2.147 2.129 -0.060
ENSG00000184178 E003 40.007786 0.0005353163 5.018845e-06 3.571636e-05 4 52873788 52873815 28 - 1.347 1.639 1.003
ENSG00000184178 E004 166.587216 0.0003318457 2.470454e-07 2.325913e-06 4 52873816 52874061 246 - 2.074 2.224 0.502
ENSG00000184178 E005 133.762352 0.0020396323 1.271369e-03 4.881663e-03 4 52885747 52885866 120 - 2.003 2.121 0.395
ENSG00000184178 E006 130.838461 0.0002511654 1.752384e-02 4.609222e-02 4 52907457 52907591 135 - 2.028 2.100 0.241
ENSG00000184178 E007 136.542239 0.0003233000 9.285482e-01 9.590332e-01 4 52920725 52920870 146 - 2.101 2.097 -0.014
ENSG00000184178 E008 158.266496 0.0015156477 1.100112e-01 2.055537e-01 4 53145333 53145582 250 - 2.128 2.174 0.154
ENSG00000184178 E009 125.116960 0.0002764688 1.164952e-01 2.149489e-01 4 53273826 53274001 176 - 2.102 2.044 -0.194
ENSG00000184178 E010 113.520213 0.0038221596 5.857610e-02 1.241866e-01 4 53313636 53313763 128 - 2.083 1.989 -0.313
ENSG00000184178 E011 115.389366 0.0047309344 1.249321e-01 2.270312e-01 4 53352598 53352766 169 - 2.081 1.998 -0.279
ENSG00000184178 E012 133.024544 0.0002848339 3.881676e-02 8.884415e-02 4 53365104 53365395 292 - 2.137 2.064 -0.242
ENSG00000184178 E013 188.796111 0.0032977303 1.705029e-04 8.372539e-04 4 53365396 53365998 603 - 2.333 2.188 -0.485
ENSG00000184178 E014 37.997476 0.0005761156 6.736473e-03 2.053924e-02 4 53365999 53366137 139 - 1.661 1.498 -0.557