ENSG00000183873

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000327956 ENSG00000183873 HEK293_OSMI2_2hA HEK293_TMG_2hB SCN5A protein_coding protein_coding 4.399748 8.828731 2.801557 0.7400972 0.153133 -1.65247 0.15731175 0.5517150 0.00000000 0.5517150 0.00000000 -5.8117664 0.01599167 0.05396667 0.00000000 -0.05396667 9.630765e-01 8.010708e-12 FALSE FALSE
ENST00000413689 ENSG00000183873 HEK293_OSMI2_2hA HEK293_TMG_2hB SCN5A protein_coding protein_coding 4.399748 8.828731 2.801557 0.7400972 0.153133 -1.65247 0.26350757 0.1859452 0.34195500 0.1140891 0.26579862 0.8449407 0.09825000 0.02070000 0.13183333 0.11113333 7.185396e-01 8.010708e-12 FALSE TRUE
ENST00000476683 ENSG00000183873 HEK293_OSMI2_2hA HEK293_TMG_2hB SCN5A protein_coding retained_intron 4.399748 8.828731 2.801557 0.7400972 0.153133 -1.65247 1.24400317 3.9482398 0.61534306 0.2002831 0.08869086 -2.6621392 0.23310000 0.45490000 0.22120000 -0.23370000 1.380860e-02 8.010708e-12 FALSE TRUE
ENST00000491944 ENSG00000183873 HEK293_OSMI2_2hA HEK293_TMG_2hB SCN5A protein_coding retained_intron 4.399748 8.828731 2.801557 0.7400972 0.153133 -1.65247 0.91270623 2.0926657 0.02494604 0.1349395 0.02494604 -5.9109466 0.12105417 0.23870000 0.00970000 -0.22900000 8.010708e-12 8.010708e-12 FALSE TRUE
MSTRG.22738.1 ENSG00000183873 HEK293_OSMI2_2hA HEK293_TMG_2hB SCN5A protein_coding   4.399748 8.828731 2.801557 0.7400972 0.153133 -1.65247 1.41384945 1.5545498 1.34543636 0.2562351 0.28040833 -0.2069902 0.39617083 0.17373333 0.47540000 0.30166667 5.792093e-03 8.010708e-12 FALSE TRUE
MSTRG.22738.11 ENSG00000183873 HEK293_OSMI2_2hA HEK293_TMG_2hB SCN5A protein_coding   4.399748 8.828731 2.801557 0.7400972 0.153133 -1.65247 0.05517362 0.0000000 0.20076780 0.0000000 0.10400280 4.3975825 0.02154583 0.00000000 0.07053333 0.07053333 5.418281e-02 8.010708e-12 FALSE TRUE
MSTRG.22738.8 ENSG00000183873 HEK293_OSMI2_2hA HEK293_TMG_2hB SCN5A protein_coding   4.399748 8.828731 2.801557 0.7400972 0.153133 -1.65247 0.06008859 0.0000000 0.21364062 0.0000000 0.21364062 4.4831103 0.02005833 0.00000000 0.06913333 0.06913333 6.371597e-01 8.010708e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000183873 E001 0.0000000       3 38547939 38548056 118 -      
ENSG00000183873 E002 0.1817044 0.0396818385 7.242065e-01   3 38548057 38548061 5 - 0.000 0.103 8.616
ENSG00000183873 E003 81.9861645 0.0006180874 2.378915e-04 1.124559e-03 3 38548062 38549127 1066 - 1.777 1.947 0.572
ENSG00000183873 E004 66.3445133 0.0010392880 9.401698e-01 9.664157e-01 3 38549128 38549967 840 - 1.797 1.811 0.048
ENSG00000183873 E005 29.7048838 0.0007194454 3.886085e-01 5.326883e-01 3 38549968 38550320 353 - 1.416 1.489 0.252
ENSG00000183873 E006 80.7507372 0.0004563934 1.775542e-03 6.520967e-03 3 38550321 38551501 1181 - 1.955 1.850 -0.352
ENSG00000183873 E007 12.1350567 0.0015358835 5.728617e-01 6.989067e-01 3 38551502 38551558 57 - 1.125 1.086 -0.140
ENSG00000183873 E008 1.2910213 0.0120242662 5.610462e-01 6.889494e-01 3 38552981 38553300 320 - 0.254 0.368 0.746
ENSG00000183873 E009 16.6057156 0.0013372383 4.086681e-02 9.264758e-02 3 38554279 38554374 96 - 1.311 1.156 -0.548
ENSG00000183873 E010 21.4260087 0.0011933904 2.377589e-04 1.124073e-03 3 38554375 38554549 175 - 1.472 1.216 -0.889
ENSG00000183873 E011 15.7548066 0.0203434109 2.977553e-02 7.152131e-02 3 38555656 38555760 105 - 1.320 1.110 -0.741
ENSG00000183873 E012 14.6955092 0.0013006203 4.248119e-02 9.559365e-02 3 38556441 38556578 138 - 1.267 1.105 -0.577
ENSG00000183873 E013 9.2602805 0.0019019057 1.076191e-02 3.059487e-02 3 38557231 38557284 54 - 1.138 0.884 -0.934
ENSG00000183873 E014 20.8710814 0.0126804718 1.663449e-03 6.164740e-03 3 38560147 38560428 282 - 1.477 1.208 -0.939
ENSG00000183873 E015 13.1278486 0.0013919292 9.322106e-03 2.708824e-02 3 38562415 38562537 123 - 1.258 1.036 -0.795
ENSG00000183873 E016 16.5852975 0.0016383248 1.021668e-02 2.929897e-02 3 38566409 38566582 174 - 1.344 1.148 -0.692
ENSG00000183873 E017 14.8091869 0.0012490663 1.728413e-02 4.557453e-02 3 38575297 38575451 155 - 1.294 1.104 -0.674
ENSG00000183873 E018 11.4084692 0.0017900636 7.017890e-02 1.436587e-01 3 38576661 38576781 121 - 1.174 1.014 -0.578
ENSG00000183873 E019 3.9957268 0.0858327125 9.197109e-01 9.532788e-01 3 38579334 38579492 159 - 0.696 0.693 -0.013
ENSG00000183873 E020 0.6998377 0.0167083513 6.644182e-01 7.722379e-01 3 38579493 38579495 3 - 0.254 0.186 -0.576
ENSG00000183873 E021 20.5780953 0.0009077445 3.167477e-04 1.447653e-03 3 38580931 38581371 441 - 1.460 1.209 -0.875
ENSG00000183873 E022 25.0812897 0.0144995020 1.058778e-03 4.162493e-03 3 38585691 38586041 351 - 1.552 1.286 -0.919
ENSG00000183873 E023 15.3078724 0.0185256427 6.117278e-04 2.576135e-03 3 38587400 38587573 174 - 1.382 1.046 -1.191
ENSG00000183873 E024 18.9221507 0.0012648329 1.177771e-03 4.565970e-03 3 38597729 38597967 239 - 1.416 1.180 -0.827
ENSG00000183873 E025 11.1471178 0.0107209932 2.459583e-02 6.104475e-02 3 38598918 38599050 133 - 1.196 0.967 -0.829
ENSG00000183873 E026 28.9125556 0.0007881827 3.822890e-04 1.706210e-03 3 38603712 38604083 372 - 1.575 1.364 -0.726
ENSG00000183873 E027 19.7518305 0.0011419577 8.467254e-03 2.495404e-02 3 38604729 38604908 180 - 1.402 1.216 -0.650
ENSG00000183873 E028 22.3994178 0.0008512747 1.332212e-04 6.731844e-04 3 38605951 38606148 198 - 1.494 1.237 -0.895
ENSG00000183873 E029 18.2129253 0.0011064836 1.497985e-04 7.461648e-04 3 38606669 38606810 142 - 1.422 1.140 -0.993
ENSG00000183873 E030 8.6208495 0.0019722206 3.308781e-03 1.117320e-02 3 38608151 38608214 64 - 1.138 0.836 -1.119
ENSG00000183873 E031 16.2732581 0.0024617551 7.110998e-04 2.938504e-03 3 38609734 38609964 231 - 1.374 1.104 -0.952
ENSG00000183873 E032 0.1482932 0.0420038304 7.249465e-01   3 38613743 38613834 92 - 0.000 0.102 11.063
ENSG00000183873 E033 6.0378280 0.0061030390 7.733660e-01 8.537640e-01 3 38613975 38614027 53 - 0.843 0.819 -0.092
ENSG00000183873 E034 8.3168605 0.0021358204 1.208821e-02 3.374558e-02 3 38614028 38614066 39 - 1.098 0.836 -0.976
ENSG00000183873 E035 0.9566701 0.2427764635 8.246659e-01 8.895539e-01 3 38614067 38614603 537 - 0.254 0.311 0.398
ENSG00000183873 E036 11.8114549 0.0018427491 1.169412e-02 3.280592e-02 3 38620843 38620971 129 - 1.218 0.991 -0.819
ENSG00000183873 E037 11.9078434 0.0014907287 1.696939e-03 6.273513e-03 3 38622400 38622489 90 - 1.258 0.979 -1.005
ENSG00000183873 E038 11.0533076 0.0018148573 9.885313e-02 1.887275e-01 3 38629842 38630310 469 - 0.932 1.122 0.697
ENSG00000183873 E039 20.4273057 0.0010497411 2.384829e-02 5.949574e-02 3 38630311 38630429 119 - 1.409 1.256 -0.533
ENSG00000183873 E040 103.2935440 0.0090043185 2.667952e-17 1.230968e-15 3 38633035 38633110 76 - 1.593 2.127 1.802
ENSG00000183873 E041 135.4675714 0.0003909742 3.431567e-47 1.800831e-44 3 38633111 38633359 249 - 1.702 2.241 1.812
ENSG00000183873 E042 0.0000000       3 38645691 38645776 86 -      
ENSG00000183873 E043 45.5091749 0.0005752640 1.178606e-09 1.722733e-08 3 38649531 38649737 207 - 1.367 1.743 1.288