ENSG00000183853

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000359209 ENSG00000183853 HEK293_OSMI2_2hA HEK293_TMG_2hB KIRREL1 protein_coding protein_coding 7.521964 7.657245 7.973783 0.4862714 0.1754318 0.05836425 1.8763242 2.32613528 1.481637 0.32089086 0.30538943 -0.647227 0.25160417 0.301300000 0.18650000 -0.11480000 1.748968e-01 3.762146e-09 FALSE TRUE
ENST00000368172 ENSG00000183853 HEK293_OSMI2_2hA HEK293_TMG_2hB KIRREL1 protein_coding protein_coding 7.521964 7.657245 7.973783 0.4862714 0.1754318 0.05836425 1.9136319 4.07226772 0.565700 0.13722646 0.56570002 -2.825982 0.25190833 0.536866667 0.06796667 -0.46890000 3.436896e-02 3.762146e-09 FALSE TRUE
ENST00000368173 ENSG00000183853 HEK293_OSMI2_2hA HEK293_TMG_2hB KIRREL1 protein_coding protein_coding 7.521964 7.657245 7.973783 0.4862714 0.1754318 0.05836425 0.2190702 0.01510135 0.516188 0.01510135 0.08865718 4.389741 0.02842083 0.001766667 0.06460000 0.06283333 3.912892e-07 3.762146e-09 FALSE TRUE
MSTRG.2402.3 ENSG00000183853 HEK293_OSMI2_2hA HEK293_TMG_2hB KIRREL1 protein_coding   7.521964 7.657245 7.973783 0.4862714 0.1754318 0.05836425 3.3969533 1.17082231 5.410257 0.26195902 0.35943972 2.198569 0.45091667 0.149833333 0.68093333 0.53110000 3.762146e-09 3.762146e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000183853 E001 2.0316491 0.0071087640 1.374042e-02 3.763101e-02 1 157993273 157993574 302 + 0.590 0.257 -1.840
ENSG00000183853 E002 6.3294407 0.0049026928 6.451477e-03 1.979709e-02 1 157993575 157993644 70 + 0.948 0.735 -0.829
ENSG00000183853 E003 17.1169470 0.0220135783 1.304483e-03 4.992030e-03 1 157993645 157993728 84 + 1.332 1.132 -0.707
ENSG00000183853 E004 0.2998086 0.0295755746 9.601130e-01   1 157993729 157993732 4 + 0.101 0.147 0.616
ENSG00000183853 E005 31.5855006 0.0079893762 6.376436e-03 1.960089e-02 1 158076113 158076262 150 + 1.529 1.479 -0.171
ENSG00000183853 E006 35.4666303 0.0224442455 9.270372e-02 1.793513e-01 1 158077991 158078140 150 + 1.555 1.554 -0.003
ENSG00000183853 E007 48.0435798 0.0022500435 6.671392e-06 4.617166e-05 1 158084422 158084579 158 + 1.724 1.629 -0.322
ENSG00000183853 E008 42.4834117 0.0005049101 3.674802e-06 2.697746e-05 1 158086596 158086679 84 + 1.674 1.576 -0.333
ENSG00000183853 E009 41.4946431 0.0005469442 4.074452e-09 5.393326e-08 1 158086680 158086746 67 + 1.691 1.517 -0.593
ENSG00000183853 E010 50.4619203 0.0006612471 1.195434e-07 1.197058e-06 1 158087755 158087860 106 + 1.751 1.637 -0.387
ENSG00000183853 E011 53.1398591 0.0011904448 6.523250e-06 4.524457e-05 1 158088006 158088154 149 + 1.759 1.687 -0.246
ENSG00000183853 E012 50.1049078 0.0004637364 3.327789e-03 1.122838e-02 1 158088327 158088454 128 + 1.700 1.708 0.026
ENSG00000183853 E013 21.3120125 0.0010807658 2.481842e-01 3.837870e-01 1 158089454 158089501 48 + 1.310 1.373 0.217
ENSG00000183853 E014 43.5102655 0.0042305623 9.808741e-02 1.876040e-01 1 158089502 158089628 127 + 1.620 1.672 0.176
ENSG00000183853 E015 29.3149745 0.0065424855 1.953172e-01 3.203687e-01 1 158089718 158089776 59 + 1.452 1.506 0.185
ENSG00000183853 E016 28.3731340 0.0019658492 6.250013e-03 1.926971e-02 1 158089777 158089818 42 + 1.476 1.441 -0.117
ENSG00000183853 E017 63.3148481 0.0093705951 1.690342e-03 6.252419e-03 1 158091358 158091556 199 + 1.823 1.777 -0.156
ENSG00000183853 E018 38.9726884 0.0092337946 3.023338e-02 7.242914e-02 1 158093339 158093446 108 + 1.598 1.599 0.005
ENSG00000183853 E019 52.3783676 0.0184762171 1.483651e-02 4.012122e-02 1 158093623 158093762 140 + 1.737 1.704 -0.110
ENSG00000183853 E020 36.2231055 0.0285806106 4.158892e-02 9.397986e-02 1 158094313 158094390 78 + 1.580 1.548 -0.110
ENSG00000183853 E021 1266.8470472 0.0084394287 5.882715e-11 1.077921e-09 1 158094644 158100262 5619 + 2.960 3.230 0.898