ENSG00000183765

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000348295 ENSG00000183765 HEK293_OSMI2_2hA HEK293_TMG_2hB CHEK2 protein_coding protein_coding 33.46245 28.00778 37.2854 4.770907 1.1979 0.4126552 3.195540 2.935141 2.786208 0.2678622 0.2923509 -0.07486504 0.09639583 0.1074333 0.07473333 -0.03270000 1.952879e-01 1.830306e-64 FALSE TRUE
ENST00000404276 ENSG00000183765 HEK293_OSMI2_2hA HEK293_TMG_2hB CHEK2 protein_coding protein_coding 33.46245 28.00778 37.2854 4.770907 1.1979 0.4126552 1.719087 0.000000 4.655345 0.0000000 0.6491810 8.86583994 0.04631667 0.0000000 0.12596667 0.12596667 6.183280e-21 1.830306e-64 FALSE TRUE
ENST00000417588 ENSG00000183765 HEK293_OSMI2_2hA HEK293_TMG_2hB CHEK2 protein_coding nonsense_mediated_decay 33.46245 28.00778 37.2854 4.770907 1.1979 0.4126552 2.948907 1.271200 4.001470 0.7253669 1.0853208 1.64663514 0.08175417 0.0404000 0.10840000 0.06800000 5.215691e-01 1.830306e-64 FALSE TRUE
ENST00000433728 ENSG00000183765 HEK293_OSMI2_2hA HEK293_TMG_2hB CHEK2 protein_coding nonsense_mediated_decay 33.46245 28.00778 37.2854 4.770907 1.1979 0.4126552 13.028443 10.200919 14.815176 1.3542210 1.2935825 0.53793660 0.38641250 0.3687667 0.39693333 0.02816667 8.094611e-01 1.830306e-64 TRUE TRUE
ENST00000434810 ENSG00000183765 HEK293_OSMI2_2hA HEK293_TMG_2hB CHEK2 protein_coding protein_coding 33.46245 28.00778 37.2854 4.770907 1.1979 0.4126552 2.117603 3.007613 1.732981 0.9536655 0.4837472 -0.79185091 0.06436250 0.1042667 0.04580000 -0.05846667 1.286812e-01 1.830306e-64 FALSE TRUE
ENST00000464581 ENSG00000183765 HEK293_OSMI2_2hA HEK293_TMG_2hB CHEK2 protein_coding protein_coding 33.46245 28.00778 37.2854 4.770907 1.1979 0.4126552 2.800291 5.274418 0.000000 0.7131275 0.0000000 -9.04560081 0.09400417 0.1903667 0.00000000 -0.19036667 1.830306e-64 1.830306e-64 FALSE TRUE
ENST00000472807 ENSG00000183765 HEK293_OSMI2_2hA HEK293_TMG_2hB CHEK2 protein_coding retained_intron 33.46245 28.00778 37.2854 4.770907 1.1979 0.4126552 2.390493 2.522234 2.483306 0.9585478 0.2759093 -0.02235062 0.07731667 0.0848000 0.06630000 -0.01850000 7.315189e-01 1.830306e-64   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000183765 E001 4.7797523 0.1243021116 3.942669e-01 5.381240e-01 22 28687743 28687743 1 - 0.658 0.835 0.718
ENSG00000183765 E002 7.1098726 0.0528979520 1.536419e-01 2.666257e-01 22 28687744 28687748 5 - 0.779 1.012 0.886
ENSG00000183765 E003 7.4430924 0.0352013692 1.177342e-01 2.166990e-01 22 28687749 28687750 2 - 0.800 1.027 0.861
ENSG00000183765 E004 14.7050572 0.0185438529 8.597663e-03 2.528253e-02 22 28687751 28687761 11 - 1.031 1.322 1.038
ENSG00000183765 E005 14.7050572 0.0185438529 8.597663e-03 2.528253e-02 22 28687762 28687762 1 - 1.031 1.322 1.038
ENSG00000183765 E006 14.7050572 0.0185438529 8.597663e-03 2.528253e-02 22 28687763 28687765 3 - 1.031 1.322 1.038
ENSG00000183765 E007 82.7201858 0.0003598151 6.466114e-07 5.593288e-06 22 28687766 28687886 121 - 1.822 2.021 0.669
ENSG00000183765 E008 74.4909421 0.0004447634 1.600235e-05 1.014795e-04 22 28687887 28687896 10 - 1.788 1.971 0.615
ENSG00000183765 E009 159.5473965 0.0063695410 5.617636e-04 2.391238e-03 22 28687897 28687986 90 - 2.119 2.289 0.568
ENSG00000183765 E010 187.5410947 0.0041765885 4.842851e-05 2.738371e-04 22 28689135 28689215 81 - 2.190 2.359 0.565
ENSG00000183765 E011 18.0022541 0.0169835892 6.594544e-01 7.682712e-01 22 28689216 28689410 195 - 1.258 1.324 0.231
ENSG00000183765 E012 213.3996166 0.0014221327 1.325438e-04 6.700654e-04 22 28694032 28694117 86 - 2.277 2.395 0.394
ENSG00000183765 E013 230.5668344 0.0001808819 1.119627e-06 9.200571e-06 22 28695127 28695209 83 - 2.310 2.427 0.392
ENSG00000183765 E014 156.7661339 0.0002200316 9.763821e-03 2.818164e-02 22 28695210 28695242 33 - 2.166 2.244 0.260
ENSG00000183765 E015 265.4449707 0.0001719567 2.821695e-04 1.307237e-03 22 28695710 28695873 164 - 2.390 2.473 0.276
ENSG00000183765 E016 74.3922792 0.0003555067 8.232158e-01 8.885964e-01 22 28696901 28696901 1 - 1.875 1.889 0.048
ENSG00000183765 E017 110.5772275 0.0002626382 2.656245e-01 4.036970e-01 22 28696902 28696943 42 - 2.032 2.075 0.142
ENSG00000183765 E018 120.2669867 0.0002676587 6.609547e-01 7.695459e-01 22 28696944 28696987 44 - 2.083 2.102 0.066
ENSG00000183765 E019 6.1944022 0.0052391470 7.336760e-05 3.963746e-04 22 28699764 28699837 74 - 1.042 0.499 -2.217
ENSG00000183765 E020 173.9807922 0.0011347976 8.179626e-01 8.849023e-01 22 28699838 28699937 100 - 2.251 2.244 -0.021
ENSG00000183765 E021 97.5357653 0.0030442245 5.127676e-01 6.476685e-01 22 28703505 28703566 62 - 2.013 1.984 -0.099
ENSG00000183765 E022 115.2436199 0.0087038450 3.914011e-01 5.353117e-01 22 28710006 28710059 54 - 2.095 2.042 -0.176
ENSG00000183765 E023 169.7420242 0.0027025271 4.410266e-02 9.857148e-02 22 28711909 28712017 109 - 2.272 2.196 -0.256
ENSG00000183765 E024 0.5954526 0.0172671820 5.870341e-02 1.244106e-01 22 28712018 28712111 94 - 0.338 0.000 -12.590
ENSG00000183765 E025 0.0000000       22 28712112 28712257 146 -      
ENSG00000183765 E026 0.0000000       22 28712261 28712495 235 -      
ENSG00000183765 E027 140.7768333 0.0047762897 4.394296e-02 9.828065e-02 22 28719395 28719485 91 - 2.199 2.104 -0.319
ENSG00000183765 E028 2.2195919 0.0075597509 2.475219e-01 3.830380e-01 22 28721518 28721631 114 - 0.393 0.588 0.964
ENSG00000183765 E029 1.8005608 0.1462836685 3.518159e-01 4.960219e-01 22 28724565 28724885 321 - 0.339 0.544 1.082
ENSG00000183765 E030 1.9971051 0.0076400761 1.001728e-01 1.907396e-01 22 28724886 28724923 38 - 0.596 0.318 -1.452
ENSG00000183765 E031 0.0000000       22 28724965 28724976 12 -      
ENSG00000183765 E032 152.1862645 0.0002456120 1.383634e-04 6.963825e-04 22 28724977 28725040 64 - 2.240 2.136 -0.349
ENSG00000183765 E033 141.9129094 0.0002831645 5.385495e-04 2.304279e-03 22 28725041 28725082 42 - 2.208 2.110 -0.328
ENSG00000183765 E034 132.3527117 0.0002849176 1.353029e-04 6.825564e-04 22 28725083 28725124 42 - 2.185 2.073 -0.375
ENSG00000183765 E035 242.6000276 0.0001838882 7.564571e-10 1.147662e-08 22 28725243 28725367 125 - 2.456 2.322 -0.446
ENSG00000183765 E036 0.1482932 0.0411597534 4.041902e-01   22 28726245 28726361 117 - 0.000 0.133 10.882
ENSG00000183765 E037 0.1451727 0.0444032056 6.033168e-01   22 28727980 28728122 143 - 0.112 0.000 -10.589
ENSG00000183765 E038 0.3697384 0.0248826798 1.526942e-01 2.653359e-01 22 28729282 28729376 95 - 0.000 0.235 11.886
ENSG00000183765 E039 6.4612851 0.0025073356 2.619182e-01 3.996425e-01 22 28730420 28730455 36 - 0.938 0.805 -0.509
ENSG00000183765 E040 13.0183713 0.0031547652 1.230609e-01 2.243195e-01 22 28730456 28730548 93 - 1.213 1.071 -0.509
ENSG00000183765 E041 276.1151106 0.0014984499 6.955967e-08 7.283006e-07 22 28734403 28734727 325 - 2.514 2.368 -0.488
ENSG00000183765 E042 2.1400852 0.0088374480 9.506924e-01 9.731400e-01 22 28737260 28737323 64 - 0.486 0.499 0.063
ENSG00000183765 E043 1.7036236 0.0262369819 7.683103e-01 8.503030e-01 22 28737885 28737915 31 - 0.393 0.447 0.289
ENSG00000183765 E044 3.4020529 0.0178850948 1.428323e-01 2.518958e-01 22 28737916 28738038 123 - 0.734 0.500 -1.032
ENSG00000183765 E045 3.5794934 0.0045104504 5.291208e-03 1.672484e-02 22 28738082 28738197 116 - 0.820 0.387 -1.962
ENSG00000183765 E046 82.5642041 0.0012192284 1.360646e-06 1.097923e-05 22 28741769 28741781 13 - 2.013 1.812 -0.675
ENSG00000183765 E047 73.5474383 0.0003746474 4.158992e-06 3.012344e-05 22 28741782 28742422 641 - 1.959 1.772 -0.628