ENSG00000183666

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000652767 ENSG00000183666 HEK293_OSMI2_2hA HEK293_TMG_2hB GUSBP1 transcribed_unprocessed_pseudogene processed_transcript 9.388033 8.427955 11.73255 1.253503 0.5521839 0.4767803 0.3903205 0.60857019 0.05663322 0.26145708 0.0283567 -3.2146238 0.04685833 0.06963333 0.00460000 -0.06503333 0.002208536 0.00177511    
ENST00000685140 ENSG00000183666 HEK293_OSMI2_2hA HEK293_TMG_2hB GUSBP1 transcribed_unprocessed_pseudogene processed_transcript 9.388033 8.427955 11.73255 1.253503 0.5521839 0.4767803 2.3439799 2.18935093 3.24167916 0.30334715 0.1045713 0.5641071 0.24275417 0.26066667 0.27766667 0.01700000 0.855302006 0.00177511 FALSE  
ENST00000685932 ENSG00000183666 HEK293_OSMI2_2hA HEK293_TMG_2hB GUSBP1 transcribed_unprocessed_pseudogene processed_transcript 9.388033 8.427955 11.73255 1.253503 0.5521839 0.4767803 1.2098512 0.88257860 1.18031363 0.44394919 0.1397896 0.4152906 0.13797917 0.12383333 0.10023333 -0.02360000 0.945775247 0.00177511    
ENST00000686303 ENSG00000183666 HEK293_OSMI2_2hA HEK293_TMG_2hB GUSBP1 transcribed_unprocessed_pseudogene processed_transcript 9.388033 8.427955 11.73255 1.253503 0.5521839 0.4767803 0.5168776 0.67720962 0.47028414 0.12182753 0.1061487 -0.5168620 0.05757083 0.08000000 0.04103333 -0.03896667 0.166084780 0.00177511    
ENST00000691699 ENSG00000183666 HEK293_OSMI2_2hA HEK293_TMG_2hB GUSBP1 transcribed_unprocessed_pseudogene processed_transcript 9.388033 8.427955 11.73255 1.253503 0.5521839 0.4767803 1.9682896 2.52532918 2.49747909 0.79954850 0.1837203 -0.0159354 0.20875833 0.28460000 0.21383333 -0.07076667 0.560120525 0.00177511    
MSTRG.25978.10 ENSG00000183666 HEK293_OSMI2_2hA HEK293_TMG_2hB GUSBP1 transcribed_unprocessed_pseudogene   9.388033 8.427955 11.73255 1.253503 0.5521839 0.4767803 0.5527779 0.17876964 0.74885876 0.02195880 0.0463787 2.0072046 0.05445417 0.02283333 0.06440000 0.04156667 0.006647975 0.00177511 TRUE  
MSTRG.25978.12 ENSG00000183666 HEK293_OSMI2_2hA HEK293_TMG_2hB GUSBP1 transcribed_unprocessed_pseudogene   9.388033 8.427955 11.73255 1.253503 0.5521839 0.4767803 0.4661168 0.05845576 0.66972835 0.05845576 0.3450469 3.3117144 0.04767917 0.00900000 0.05963333 0.05063333 0.541932790 0.00177511 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000183666 E001 0.0000000       5 21341833 21341976 144 +      
ENSG00000183666 E002 0.0000000       5 21405685 21405760 76 +      
ENSG00000183666 E003 0.1482932 0.0411597534 3.864489e-01   5 21459452 21459479 28 + 0.000 0.133 9.371
ENSG00000183666 E004 0.1482932 0.0411597534 3.864489e-01   5 21459480 21459491 12 + 0.000 0.133 11.432
ENSG00000183666 E005 2.3561461 0.4500277145 8.421471e-01 9.015975e-01 5 21459492 21459496 5 + 0.523 0.559 0.167
ENSG00000183666 E006 2.3561461 0.4500277145 8.421471e-01 9.015975e-01 5 21459497 21459498 2 + 0.523 0.559 0.167
ENSG00000183666 E007 8.1371853 0.0751707040 9.746233e-01 9.881853e-01 5 21459499 21459512 14 + 0.978 0.970 -0.027
ENSG00000183666 E008 8.1371853 0.0751707040 9.746233e-01 9.881853e-01 5 21459513 21459514 2 + 0.978 0.970 -0.027
ENSG00000183666 E009 8.4306512 0.0908742719 9.719070e-01 9.864343e-01 5 21459515 21459519 5 + 0.991 0.983 -0.026
ENSG00000183666 E010 11.9863209 0.0294836332 7.973793e-01 8.707044e-01 5 21459520 21459524 5 + 1.143 1.104 -0.143
ENSG00000183666 E011 17.3493175 0.0012031437 7.152433e-01 8.112017e-01 5 21459525 21459530 6 + 1.263 1.279 0.056
ENSG00000183666 E012 30.6427895 0.0007370554 5.306656e-01 6.632253e-01 5 21459531 21459555 25 + 1.495 1.519 0.084
ENSG00000183666 E013 37.5685089 0.0006363068 8.025360e-01 8.742246e-01 5 21459556 21459584 29 + 1.593 1.593 0.001
ENSG00000183666 E014 1.0351233 0.0119934522 8.995957e-03 2.626905e-02 5 21459585 21459677 93 + 0.485 0.000 -14.762
ENSG00000183666 E015 53.2922405 0.0005141382 5.527169e-01 6.819536e-01 5 21459678 21459743 66 + 1.759 1.717 -0.140
ENSG00000183666 E016 0.1515154 0.0454745477 6.270157e-01   5 21459744 21459849 106 + 0.112 0.000 -11.961
ENSG00000183666 E017 65.6783541 0.0005151827 4.651513e-01 6.048646e-01 5 21461783 21461866 84 + 1.852 1.809 -0.147
ENSG00000183666 E018 0.8491631 0.0165869916 8.602524e-01 9.138581e-01 5 21461867 21461990 124 + 0.274 0.236 -0.287
ENSG00000183666 E019 16.9540078 0.0479384094 6.928679e-01 7.939758e-01 5 21466619 21466748 130 + 1.234 1.295 0.214
ENSG00000183666 E020 8.6751871 0.0022823109 2.127291e-01 3.417559e-01 5 21466749 21466977 229 + 0.936 1.058 0.451
ENSG00000183666 E021 9.8091789 0.0019121334 1.081215e-02 3.071418e-02 5 21470510 21470662 153 + 0.906 1.156 0.919
ENSG00000183666 E022 4.1400138 0.0606685072 4.646906e-05 2.638362e-04 5 21470982 21471038 57 + 0.201 0.959 3.787
ENSG00000183666 E023 4.5023653 0.0641259698 1.288547e-04 6.538812e-04 5 21471039 21471172 134 + 0.274 0.977 3.268
ENSG00000183666 E024 18.3360323 0.0047439799 1.139219e-01 2.112398e-01 5 21471462 21471601 140 + 1.227 1.341 0.399
ENSG00000183666 E025 10.3546688 0.0020602169 2.873818e-02 6.947973e-02 5 21471602 21471646 45 + 0.936 1.146 0.766
ENSG00000183666 E026 3.3129020 0.0049786031 1.596126e-01 2.746013e-01 5 21473283 21473356 74 + 0.525 0.726 0.881
ENSG00000183666 E027 176.6661409 0.0002376464 3.471064e-08 3.861092e-07 5 21473357 21474717 1361 + 2.198 2.309 0.370
ENSG00000183666 E028 7.4609961 0.0028125231 9.595581e-03 2.776891e-02 5 21491321 21491508 188 + 1.052 0.727 -1.247
ENSG00000183666 E029 7.2290173 0.0954243448 2.188455e-02 5.543545e-02 5 21495098 21495182 85 + 1.074 0.635 -1.712
ENSG00000183666 E030 10.1568711 0.0018717067 5.321112e-05 2.978046e-04 5 21497022 21497196 175 + 1.211 0.755 -1.703
ENSG00000183666 E031 11.0408829 0.0243192886 1.042501e-03 4.105832e-03 5 21501692 21501826 135 + 1.242 0.807 -1.604
ENSG00000183666 E032 4.7225157 0.0874268524 2.998946e-02 7.193848e-02 5 21502111 21502203 93 + 0.923 0.497 -1.784
ENSG00000183666 E033 1.3307647 0.0140275119 2.743879e-01 4.135300e-01 5 21502204 21502309 106 + 0.441 0.236 -1.286
ENSG00000183666 E034 0.5943067 0.0212004040 4.374405e-01 5.794014e-01 5 21505408 21505500 93 + 0.274 0.134 -1.291
ENSG00000183666 E035 1.6596189 0.0098756801 7.271292e-02 1.478633e-01 5 21510177 21510907 731 + 0.561 0.236 -1.875
ENSG00000183666 E036 6.1775641 0.0046004184 1.164128e-04 5.974278e-04 5 21522338 21523805 1468 + 1.041 0.500 -2.206
ENSG00000183666 E037 0.0000000       5 21544719 21544777 59 +      
ENSG00000183666 E038 42.6148789 0.0196193212 4.087209e-07 3.678954e-06 5 21585530 21589372 3843 + 1.809 1.343 -1.593