ENSG00000183648

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000605997 ENSG00000183648 HEK293_OSMI2_2hA HEK293_TMG_2hB NDUFB1 protein_coding protein_coding 189.972 288.9252 167.3742 42.14548 0.8230427 -0.7875826 115.46997 241.45140 22.89817 73.06729 5.922755 -3.397859 0.5585917 0.7980333 0.1369667 -0.6610667 0.003325479 0.0003397884 FALSE FALSE
ENST00000617122 ENSG00000183648 HEK293_OSMI2_2hA HEK293_TMG_2hB NDUFB1 protein_coding protein_coding 189.972 288.9252 167.3742 42.14548 0.8230427 -0.7875826 64.62203 36.10522 132.49276 36.10522 6.937805 1.875344 0.3866667 0.1620000 0.7914000 0.6294000 0.042176009 0.0003397884 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000183648 E001 0.5953508 1.985354e-02 4.689994e-01 6.083153e-01 14 92116059 92116121 63 - 0.285 0.163 -1.023
ENSG00000183648 E002 0.2987644 2.732023e-02 7.003129e-02   14 92116122 92116122 1 - 0.285 0.000 -10.903
ENSG00000183648 E003 16.5540175 2.781435e-02 2.233488e-05 1.369345e-04 14 92116123 92116125 3 - 0.714 1.326 2.276
ENSG00000183648 E004 1238.3096541 9.957842e-04 1.667596e-05 1.052859e-04 14 92116126 92116229 104 - 3.005 3.075 0.231
ENSG00000183648 E005 2178.4101564 9.190075e-05 6.456191e-01 7.577005e-01 14 92117498 92117642 145 - 3.298 3.308 0.034
ENSG00000183648 E006 45.7986189 7.115259e-03 1.316484e-09 1.908508e-08 14 92117643 92118726 1084 - 1.871 1.478 -1.333
ENSG00000183648 E007 63.9972992 4.243388e-04 3.495464e-07 3.189967e-06 14 92118849 92118966 118 - 1.922 1.704 -0.735
ENSG00000183648 E008 3.8341452 6.903444e-02 7.618533e-02 1.534145e-01 14 92120331 92120416 86 - 0.846 0.544 -1.268
ENSG00000183648 E009 21.6297586 8.835024e-04 1.643157e-03 6.098724e-03 14 92121196 92121211 16 - 1.466 1.242 -0.781
ENSG00000183648 E010 37.4932790 1.235104e-02 8.101282e-04 3.294898e-03 14 92121212 92121399 188 - 1.715 1.454 -0.891
ENSG00000183648 E011 14.4147523 4.459483e-02 2.189906e-05 1.345063e-04 14 92121400 92121641 242 - 1.466 0.904 -2.012
ENSG00000183648 E012 1304.4218190 8.936510e-04 1.468856e-01 2.574432e-01 14 92121642 92121917 276 - 3.093 3.071 -0.073