ENSG00000183579

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000406323 ENSG00000183579 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNRF3 protein_coding protein_coding 9.48572 8.151203 10.5045 0.4590652 0.1725305 0.365527 5.597376 6.001447 4.347673 0.5158172 0.2283801 -0.4641545 0.5983917 0.7360667 0.4135667 -0.3225 2.704071e-05 2.704071e-05 FALSE TRUE
ENST00000544604 ENSG00000183579 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNRF3 protein_coding protein_coding 9.48572 8.151203 10.5045 0.4590652 0.1725305 0.365527 3.764260 2.149756 6.156831 0.3709937 0.1563523 1.5136609 0.3838250 0.2639333 0.5864333 0.3225 2.706345e-05 2.704071e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000183579 E001 50.2034882 0.0005504818 1.576581e-09 2.254008e-08 22 28883572 28884066 495 + 1.818 1.542 -0.936
ENSG00000183579 E002 0.1472490 0.0425096170 5.773589e-01   22 28890987 28891034 48 + 0.109 0.000 -9.398
ENSG00000183579 E003 0.0000000       22 28917389 28917481 93 +      
ENSG00000183579 E004 0.1451727 0.0427011227 5.778721e-01   22 28987052 28987075 24 + 0.109 0.000 -9.398
ENSG00000183579 E005 76.9353012 0.0035693500 1.524876e-03 5.717288e-03 22 28987076 28987201 126 + 1.943 1.823 -0.403
ENSG00000183579 E006 77.6682655 0.0028066587 3.712547e-03 1.233351e-02 22 29042495 29042569 75 + 1.939 1.838 -0.340
ENSG00000183579 E007 102.0709503 0.0078718920 1.183501e-01 2.175720e-01 22 29043299 29043430 132 + 2.033 1.986 -0.157
ENSG00000183579 E008 90.1803906 0.0028376068 1.681270e-02 4.452090e-02 22 29044780 29044890 111 + 1.992 1.920 -0.241
ENSG00000183579 E009 83.9399510 0.0014570950 1.400447e-01 2.480868e-01 22 29046716 29046883 168 + 1.940 1.912 -0.093
ENSG00000183579 E010 52.8853449 0.0004284233 8.607538e-03 2.530646e-02 22 29048389 29048491 103 + 1.770 1.676 -0.317
ENSG00000183579 E011 419.9471235 0.0002987083 1.446443e-01 2.543433e-01 22 29049197 29050948 1752 + 2.592 2.651 0.196
ENSG00000183579 E012 1024.5459695 0.0038535938 9.545659e-04 3.803467e-03 22 29053579 29057488 3910 + 2.962 3.056 0.312