ENSG00000183530

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000327423 ENSG00000183530 HEK293_OSMI2_2hA HEK293_TMG_2hB PRR14L protein_coding protein_coding 11.9335 10.46003 13.3037 0.8133796 0.2750968 0.3466455 8.3520559 5.469765290 10.4920257 0.712276928 0.17197130 0.9384816 0.69134583 0.5189666667 0.7889 0.26993333 1.040944e-08 6.252683e-38 FALSE TRUE
ENST00000431684 ENSG00000183530 HEK293_OSMI2_2hA HEK293_TMG_2hB PRR14L protein_coding nonsense_mediated_decay 11.9335 10.46003 13.3037 0.8133796 0.2750968 0.3466455 0.2331804 0.006307987 0.7336529 0.003477554 0.09747443 5.5109789 0.01805417 0.0006333333 0.0555 0.05486667 4.077331e-09 6.252683e-38 TRUE TRUE
ENST00000432485 ENSG00000183530 HEK293_OSMI2_2hA HEK293_TMG_2hB PRR14L protein_coding nonsense_mediated_decay 11.9335 10.46003 13.3037 0.8133796 0.2750968 0.3466455 2.1270054 4.712799169 0.0000000 0.152071358 0.00000000 -8.8834984 0.19333333 0.4558000000 0.0000 -0.45580000 6.252683e-38 6.252683e-38 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000183530 E001 0.9566832 0.0137071527 2.112551e-01 3.399818e-01 22 31676256 31677018 763 - 0.193 0.400 1.433
ENSG00000183530 E002 0.8460400 0.2592023082 3.576550e-02 8.313090e-02 22 31681347 31681349 3 - 0.000 0.412 12.453
ENSG00000183530 E003 319.4665826 0.0209002839 3.607654e-17 1.636636e-15 22 31681350 31682014 665 - 2.155 2.715 1.865
ENSG00000183530 E004 127.4204139 0.0122922456 6.303944e-13 1.598532e-11 22 31682015 31682134 120 - 1.861 2.281 1.407
ENSG00000183530 E005 650.3230376 0.0025085728 1.090224e-32 2.232862e-30 22 31682135 31683628 1494 - 2.676 2.927 0.837
ENSG00000183530 E006 417.3562346 0.0006085070 1.768340e-09 2.502665e-08 22 31683629 31685041 1413 - 2.575 2.664 0.296
ENSG00000183530 E007 246.7140954 0.0052586638 3.765966e-01 5.209225e-01 22 31685042 31685803 762 - 2.418 2.359 -0.195
ENSG00000183530 E008 43.1955361 0.0007492078 2.111653e-01 3.398888e-01 22 31688156 31688227 72 - 1.681 1.585 -0.326
ENSG00000183530 E009 54.3324683 0.0012258500 1.592196e-01 2.740782e-01 22 31701656 31701762 107 - 1.783 1.684 -0.332
ENSG00000183530 E010 53.7253969 0.0103725760 2.928434e-01 4.338615e-01 22 31703550 31703721 172 - 1.776 1.679 -0.330
ENSG00000183530 E011 0.1451727 0.0426345628 6.812447e-01   22 31704444 31704654 211 - 0.107 0.000 -9.791
ENSG00000183530 E012 40.0791657 0.0043287924 1.343014e-02 3.691671e-02 22 31704655 31704726 72 - 1.689 1.502 -0.638
ENSG00000183530 E013 865.6078417 0.0220729847 1.198686e-10 2.088737e-09 22 31712083 31716726 4644 - 3.077 2.697 -1.262
ENSG00000183530 E014 63.4855237 0.0132212927 9.335479e-15 3.083296e-13 22 31716727 31716985 259 - 2.003 1.382 -2.109
ENSG00000183530 E015 53.8631645 0.0482354588 1.704742e-07 1.656428e-06 22 31716986 31717181 196 - 1.935 1.291 -2.198
ENSG00000183530 E016 39.6239626 0.0091841670 7.385146e-15 2.482423e-13 22 31717182 31717291 110 - 1.808 1.143 -2.293
ENSG00000183530 E017 20.1567612 0.0011155804 4.539232e-11 8.483218e-10 22 31725538 31725543 6 - 1.517 0.913 -2.150
ENSG00000183530 E018 30.8074175 0.0033315152 2.472985e-15 8.886564e-14 22 31725544 31725610 67 - 1.699 1.050 -2.259
ENSG00000183530 E019 66.7632092 0.0291886512 2.586597e-08 2.950879e-07 22 31738387 31738911 525 - 2.010 1.449 -1.903
ENSG00000183530 E020 3.7479727 0.0129593599 1.177847e-01 2.167688e-01 22 31748124 31748247 124 - 0.781 0.515 -1.151
ENSG00000183530 E021 4.0664162 0.0043957237 3.666554e-01 5.110000e-01 22 31749391 31749474 84 - 0.760 0.605 -0.651
ENSG00000183530 E022 9.6440469 0.0018252689 3.554542e-03 1.188236e-02 22 31749475 31749770 296 - 1.151 0.799 -1.311
ENSG00000183530 E023 12.0055744 0.0036380188 2.107769e-08 2.446756e-07 22 31749993 31750071 79 - 1.314 0.644 -2.524
ENSG00000183530 E024 6.4709319 0.0025767058 4.530254e-06 3.257337e-05 22 31750072 31750165 94 - 1.076 0.400 -2.850