ENSG00000182827

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366812 ENSG00000182827 HEK293_OSMI2_2hA HEK293_TMG_2hB ACBD3 protein_coding protein_coding 18.30494 10.30573 29.29319 1.628044 0.9337274 1.506211 15.685592 10.16520405 23.646258 1.60343979 0.5585528 1.217164 0.90142083 0.986400000 0.8077000 -0.1787 7.421579e-23 1.304889e-28 FALSE TRUE
MSTRG.3158.2 ENSG00000182827 HEK293_OSMI2_2hA HEK293_TMG_2hB ACBD3 protein_coding   18.30494 10.30573 29.29319 1.628044 0.9337274 1.506211 2.432483 0.02042296 5.302402 0.02042296 0.3433190 7.448060 0.08833333 0.002266667 0.1807667 0.1785 1.304889e-28 1.304889e-28 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000182827 E001 771.655049 0.0109442787 1.421055e-10 2.444073e-09 1 226144679 226146390 1712 - 2.719 3.057 1.126
ENSG00000182827 E002 70.726596 0.0004050090 5.265761e-01 6.597812e-01 1 226146391 226146407 17 - 1.792 1.866 0.249
ENSG00000182827 E003 92.977333 0.0002857576 1.764381e-01 2.966108e-01 1 226146408 226146469 62 - 1.918 1.967 0.167
ENSG00000182827 E004 299.827430 0.0001867145 1.039717e-07 1.052647e-06 1 226146470 226146821 352 - 2.439 2.425 -0.048
ENSG00000182827 E005 294.091493 0.0048829644 4.814827e-05 2.724150e-04 1 226152335 226152607 273 - 2.443 2.376 -0.221
ENSG00000182827 E006 84.889633 0.0026227443 5.409851e-03 1.704072e-02 1 226152608 226152619 12 - 1.901 1.862 -0.129
ENSG00000182827 E007 1.485403 0.0090452964 1.747831e-02 4.599739e-02 1 226154642 226154646 5 - 0.456 0.001 -9.908
ENSG00000182827 E008 184.913766 0.0003347834 1.114161e-05 7.323274e-05 1 226154647 226154833 187 - 2.234 2.209 -0.084
ENSG00000182827 E009 144.936291 0.0029405594 4.998612e-01 6.361195e-01 1 226159184 226159358 175 - 2.100 2.168 0.229
ENSG00000182827 E010 175.270705 0.0002873813 8.230774e-03 2.436894e-02 1 226161531 226161689 159 - 2.195 2.221 0.085
ENSG00000182827 E011 147.159623 0.0002834844 1.121227e-08 1.369612e-07 1 226164789 226164929 141 - 2.150 2.065 -0.286
ENSG00000182827 E012 117.116315 0.0013301026 2.282807e-09 3.166602e-08 1 226165859 226166000 142 - 2.065 1.922 -0.481
ENSG00000182827 E013 85.758975 0.0112746894 2.221587e-04 1.058640e-03 1 226186390 226186741 352 - 1.932 1.769 -0.549