ENSG00000182518

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000332132 ENSG00000182518 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM104B protein_coding protein_coding 15.87894 22.75306 15.09633 2.849555 0.2424369 -0.5915409 0.9253657 1.6563247 0.2705636 0.44926645 0.13824143 -2.57026988 0.05968750 0.07310000 0.01803333 -0.05506667 2.586440e-01 5.19369e-10 FALSE TRUE
ENST00000358460 ENSG00000182518 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM104B protein_coding protein_coding 15.87894 22.75306 15.09633 2.849555 0.2424369 -0.5915409 5.1830255 8.6939632 4.6333636 0.89318191 0.16805040 -0.90650219 0.32045000 0.38603333 0.30673333 -0.07930000 2.364793e-01 5.19369e-10 FALSE TRUE
ENST00000425133 ENSG00000182518 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM104B protein_coding protein_coding 15.87894 22.75306 15.09633 2.849555 0.2424369 -0.5915409 0.6659557 0.7235397 1.2634228 0.03281076 0.08612836 0.79576447 0.04200000 0.03290000 0.08366667 0.05076667 3.384972e-04 5.19369e-10 FALSE TRUE
ENST00000472571 ENSG00000182518 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM104B protein_coding protein_coding 15.87894 22.75306 15.09633 2.849555 0.2424369 -0.5915409 1.2342685 1.7587296 0.9401964 0.36589979 0.18828737 -0.89641593 0.07762083 0.07683333 0.06256667 -0.01426667 7.509520e-01 5.19369e-10   FALSE
ENST00000477847 ENSG00000182518 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM104B protein_coding protein_coding 15.87894 22.75306 15.09633 2.849555 0.2424369 -0.5915409 1.6922195 2.1830357 2.0603281 0.32599888 0.14586104 -0.08306984 0.10882083 0.09650000 0.13646667 0.03996667 2.268340e-01 5.19369e-10 FALSE FALSE
ENST00000489298 ENSG00000182518 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM104B protein_coding protein_coding 15.87894 22.75306 15.09633 2.849555 0.2424369 -0.5915409 4.8660312 6.9315627 3.7767936 1.38363303 0.38395306 -0.87428367 0.30342083 0.29933333 0.25006667 -0.04926667 5.438412e-01 5.19369e-10 FALSE FALSE
ENST00000685693 ENSG00000182518 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM104B protein_coding protein_coding 15.87894 22.75306 15.09633 2.849555 0.2424369 -0.5915409 0.8594968 0.4741413 1.3720201 0.01444064 0.01876292 1.51327840 0.05781250 0.02146667 0.09090000 0.06943333 5.193690e-10 5.19369e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000182518 E001 156.302773 0.0023149100 1.115589e-07 1.123578e-06 X 55143102 55143875 774 - 2.049 2.241 0.642
ENSG00000182518 E002 39.469706 0.0005894535 7.726103e-24 7.540892e-22 X 55143876 55145244 1369 - 1.859 1.307 -1.887
ENSG00000182518 E003 6.422755 0.0026233528 1.103730e-07 1.112580e-06 X 55145245 55145355 111 - 1.153 0.493 -2.645
ENSG00000182518 E004 4.535221 0.0036432559 2.248091e-06 1.729413e-05 X 55145356 55145386 31 - 1.026 0.366 -2.863
ENSG00000182518 E005 30.397404 0.0007091967 9.195280e-10 1.372641e-08 X 55145387 55145937 551 - 1.680 1.307 -1.281
ENSG00000182518 E006 144.116978 0.0031014766 1.744161e-01 2.939289e-01 X 55145938 55146180 243 - 2.110 2.169 0.199
ENSG00000182518 E007 215.501932 0.0002164115 1.187453e-02 3.323034e-02 X 55146181 55146268 88 - 2.279 2.344 0.217
ENSG00000182518 E008 169.328591 0.0002442789 6.809139e-01 7.847398e-01 X 55146269 55146311 43 - 2.218 2.214 -0.015
ENSG00000182518 E009 11.011327 0.0016690624 4.556265e-01 5.961412e-01 X 55159122 55159125 4 - 1.009 1.092 0.301
ENSG00000182518 E010 26.626117 0.0012192338 9.991220e-01 1.000000e+00 X 55159126 55159128 3 - 1.419 1.425 0.021
ENSG00000182518 E011 188.990860 0.0002233836 6.740266e-01 7.795624e-01 X 55159129 55159228 100 - 2.253 2.270 0.057
ENSG00000182518 E012 7.068748 0.0023854891 7.422902e-01 8.312231e-01 X 55160743 55160864 122 - 0.915 0.881 -0.127
ENSG00000182518 E013 8.471445 0.0020988876 4.808022e-01 6.190250e-01 X 55160865 55160974 110 - 1.009 0.938 -0.267
ENSG00000182518 E014 28.270022 0.0007283695 4.133843e-01 5.568826e-01 X 55160975 55161136 162 - 1.481 1.434 -0.162
ENSG00000182518 E015 90.799921 0.0004375960 6.938898e-01 7.948379e-01 X 55161137 55161310 174 - 1.952 1.944 -0.028