ENSG00000182158

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000330387 ENSG00000182158 HEK293_OSMI2_2hA HEK293_TMG_2hB CREB3L2 protein_coding protein_coding 17.71425 18.64324 16.61349 0.5466343 0.1815217 -0.1662029 16.5884446 16.317031 15.9556127 0.3845253 0.18851605 -0.03229452 0.9365167 0.8755333 0.96040000 0.08486667 5.095747e-08 3.240977e-10 FALSE  
ENST00000458726 ENSG00000182158 HEK293_OSMI2_2hA HEK293_TMG_2hB CREB3L2 protein_coding protein_coding 17.71425 18.64324 16.61349 0.5466343 0.1815217 -0.1662029 0.9477669 2.247537 0.4328544 0.1601429 0.07670371 -2.34984528 0.0530375 0.1203000 0.02596667 -0.09433333 3.240977e-10 3.240977e-10 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000182158 E001 2676.9544986 0.0073170173 1.150925e-14 3.750891e-13 7 137874979 137879940 4962 - 3.267 3.558 0.966
ENSG00000182158 E002 214.5434238 0.0151204543 1.098766e-05 7.231725e-05 7 137879941 137880551 611 - 2.373 2.278 -0.317
ENSG00000182158 E003 106.4092514 0.0131854609 3.837078e-06 2.802865e-05 7 137882412 137882628 217 - 2.081 1.962 -0.398
ENSG00000182158 E004 0.2924217 0.0294001893 1.787742e-01   7 137884267 137884994 728 - 0.191 0.000 -14.368
ENSG00000182158 E005 77.5526089 0.0059860441 1.926049e-06 1.503375e-05 7 137884995 137885121 127 - 1.930 1.848 -0.278
ENSG00000182158 E006 69.3740522 0.0045076643 6.114681e-10 9.439438e-09 7 137885403 137885502 100 - 1.910 1.756 -0.517
ENSG00000182158 E007 55.8263872 0.0046713654 1.941295e-09 2.725654e-08 7 137901354 137901422 69 - 1.823 1.654 -0.572
ENSG00000182158 E008 60.3703823 0.0091270363 8.970542e-08 9.185182e-07 7 137903959 137904017 59 - 1.858 1.689 -0.572
ENSG00000182158 E009 75.8440239 0.0004553980 3.546858e-15 1.247571e-13 7 137905702 137905848 147 - 1.945 1.797 -0.499
ENSG00000182158 E010 75.9901028 0.0055162657 1.127809e-07 1.134799e-06 7 137908252 137908436 185 - 1.929 1.819 -0.370
ENSG00000182158 E011 0.0000000       7 137912811 137912823 13 -      
ENSG00000182158 E012 0.2965864 0.2072056661 2.768808e-01   7 137912824 137912833 10 - 0.000 0.243 14.997
ENSG00000182158 E013 16.3281131 0.0011511959 3.110526e-14 9.519869e-13 7 137912834 137912990 157 - 0.576 1.516 3.522
ENSG00000182158 E014 85.7403280 0.0041316792 6.137131e-02 1.289424e-01 7 137912991 137913078 88 - 1.892 1.977 0.288
ENSG00000182158 E015 134.3063566 0.0003100383 7.106523e-06 4.885721e-05 7 137915837 137916012 176 - 2.110 2.150 0.133
ENSG00000182158 E016 0.1817044 0.0391588248 5.245810e-01   7 137922590 137922767 178 - 0.000 0.141 14.031
ENSG00000182158 E017 114.1139865 0.0002940104 4.252829e-10 6.746091e-09 7 137928150 137928366 217 - 2.072 2.042 -0.101
ENSG00000182158 E018 0.0000000       7 137933763 137933890 128 -      
ENSG00000182158 E019 0.7061804 0.0939095176 9.502952e-01 9.728963e-01 7 137999659 137999810 152 - 0.191 0.248 0.477
ENSG00000182158 E020 141.3046061 0.0004624347 3.159775e-10 5.126401e-09 7 138001604 138002262 659 - 2.157 2.142 -0.051