ENSG00000182141

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000356929 ENSG00000182141 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF708 protein_coding protein_coding 2.429766 0.5354244 3.986605 0.1562637 0.3045851 2.873324 1.2200091 0.27535883 2.0476093 0.01241497 0.0828507 2.8501199 0.55099167 0.5903 0.51733333 -0.07296667 0.8791565855 0.0005502187 FALSE TRUE
ENST00000598046 ENSG00000182141 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF708 protein_coding nonsense_mediated_decay 2.429766 0.5354244 3.986605 0.1562637 0.3045851 2.873324 0.4870173 0.00000000 0.9985383 0.00000000 0.1833300 6.6561220 0.14487083 0.0000 0.24936667 0.24936667 0.0005502187 0.0005502187 FALSE TRUE
ENST00000601295 ENSG00000182141 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF708 protein_coding protein_coding 2.429766 0.5354244 3.986605 0.1562637 0.3045851 2.873324 0.2804955 0.09101915 0.3213818 0.09101915 0.3213818 1.7138654 0.08644583 0.1086 0.07180000 -0.03680000 0.7651412972 0.0005502187 FALSE FALSE
ENST00000602023 ENSG00000182141 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF708 protein_coding protein_coding 2.429766 0.5354244 3.986605 0.1562637 0.3045851 2.873324 0.2081930 0.05289645 0.3795408 0.02755376 0.1037189 2.6307239 0.10319167 0.1379 0.09963333 -0.03826667 0.9097766695 0.0005502187   FALSE
MSTRG.16834.4 ENSG00000182141 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF708 protein_coding   2.429766 0.5354244 3.986605 0.1562637 0.3045851 2.873324 0.2340510 0.11614995 0.2395350 0.08267647 0.1561294 0.9841024 0.11448333 0.1632 0.06183333 -0.10136667 0.6851700155 0.0005502187 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000182141 E001 38.504191 0.0008023995 2.619246e-05 0.0001580483 19 21291160 21292603 1444 - 1.398 1.635 0.811
ENSG00000182141 E002 78.551634 0.0145654199 4.328193e-03 0.0140640674 19 21292604 21294395 1792 - 1.766 1.545 -0.752
ENSG00000182141 E003 13.809844 0.0013172731 9.174349e-04 0.0036758515 19 21294396 21294542 147 - 1.070 0.446 -2.586
ENSG00000182141 E004 16.017120 0.0013043955 1.784151e-02 0.0467965000 19 21294543 21294739 197 - 1.116 0.739 -1.429
ENSG00000182141 E005 1.920818 0.0656787544 1.943155e-01 0.3191732449 19 21306818 21306941 124 - 0.315 0.569 1.345
ENSG00000182141 E006 10.285183 0.0040492879 7.801812e-01 0.8587056105 19 21309246 21309341 96 - 0.902 0.912 0.041
ENSG00000182141 E007 9.315761 0.0021189409 8.046602e-02 0.1602831193 19 21310501 21310627 127 - 0.832 1.036 0.767
ENSG00000182141 E008 3.845614 0.1179538220 8.100168e-01 0.8794149799 19 21327960 21328141 182 - 0.555 0.572 0.079
ENSG00000182141 E009 12.114423 0.0016211006 2.254619e-02 0.0568174411 19 21329210 21329425 216 - 0.921 1.160 0.876