ENSG00000182054

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000330062 ENSG00000182054 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH2 protein_coding protein_coding 89.19024 164.1227 55.79317 5.658272 0.3774685 -1.556444 12.429140 24.99045 7.040591 1.1581874 0.08607082 -1.826138 0.13320833 0.15230000 0.12620000 -0.02610000 0.083563236 0.007422959 FALSE  
ENST00000540499 ENSG00000182054 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH2 protein_coding protein_coding 89.19024 164.1227 55.79317 5.658272 0.3774685 -1.556444 44.745717 76.42920 31.371576 0.5427667 0.70668266 -1.284395 0.52433333 0.46680000 0.56250000 0.09570000 0.027697407 0.007422959 FALSE  
ENST00000560061 ENSG00000182054 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH2 protein_coding nonsense_mediated_decay 89.19024 164.1227 55.79317 5.658272 0.3774685 -1.556444 6.247973 17.25699 2.092616 1.8950227 0.49128355 -3.037759 0.07121667 0.10546667 0.03756667 -0.06790000 0.007422959 0.007422959 FALSE  
MSTRG.11521.2 ENSG00000182054 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH2 protein_coding   89.19024 164.1227 55.79317 5.658272 0.3774685 -1.556444 8.073680 9.12832 3.809666 2.1815126 0.60538119 -1.258483 0.07905833 0.05483333 0.06816667 0.01333333 0.728312807 0.007422959 FALSE  
MSTRG.11521.6 ENSG00000182054 HEK293_OSMI2_2hA HEK293_TMG_2hB IDH2 protein_coding   89.19024 164.1227 55.79317 5.658272 0.3774685 -1.556444 17.353615 35.68201 11.434862 3.1414161 0.90812414 -1.640901 0.18734583 0.21676667 0.20480000 -0.01196667 0.871671573 0.007422959 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000182054 E001 336.2277980 4.470243e-04 1.301368e-04 6.594276e-04 15 90083045 90083995 951 - 2.342 2.441 0.330
ENSG00000182054 E002 111.6817619 2.756876e-04 3.228353e-01 4.661741e-01 15 90083996 90084013 18 - 1.914 1.953 0.130
ENSG00000182054 E003 123.6980525 3.183297e-04 1.495070e-01 2.610014e-01 15 90084014 90084025 12 - 1.945 2.001 0.186
ENSG00000182054 E004 521.4033051 3.217985e-04 4.103746e-08 4.504041e-07 15 90084026 90084176 151 - 2.516 2.631 0.384
ENSG00000182054 E005 976.6437376 4.029582e-04 3.205743e-05 1.893492e-04 15 90084177 90084353 177 - 2.830 2.896 0.220
ENSG00000182054 E006 920.4822761 2.587335e-04 3.692408e-03 1.227371e-02 15 90084816 90084908 93 - 2.823 2.866 0.144
ENSG00000182054 E007 1003.0489042 1.647474e-04 9.873892e-03 2.845550e-02 15 90085001 90085098 98 - 2.867 2.902 0.115
ENSG00000182054 E008 911.3391402 1.134899e-03 3.040512e-02 7.275409e-02 15 90085275 90085387 113 - 2.819 2.862 0.144
ENSG00000182054 E009 1104.7225863 1.505567e-04 8.096334e-01 8.791460e-01 15 90087112 90087263 152 - 2.937 2.937 0.001
ENSG00000182054 E010 962.3162282 9.611927e-05 8.208400e-01 8.870528e-01 15 90087439 90087575 137 - 2.882 2.876 -0.020
ENSG00000182054 E011 1012.5781259 8.189296e-05 8.393361e-07 7.086625e-06 15 90088359 90088502 144 - 2.954 2.885 -0.227
ENSG00000182054 E012 1175.3991531 8.188853e-05 1.913836e-06 1.494705e-05 15 90088587 90088747 161 - 3.013 2.951 -0.205
ENSG00000182054 E013 0.7761102 1.832610e-02 8.912575e-01 9.345343e-01 15 90089619 90089687 69 - 0.208 0.182 -0.245
ENSG00000182054 E014 1006.4496178 5.371938e-04 1.060960e-05 7.009684e-05 15 90090479 90090644 166 - 2.956 2.880 -0.250
ENSG00000182054 E015 571.8308130 4.228178e-03 1.258404e-01 2.283468e-01 15 90091553 90091644 92 - 2.695 2.639 -0.186
ENSG00000182054 E016 0.0000000       15 90100576 90100695 120 -      
ENSG00000182054 E017 363.3379622 6.994043e-03 6.366625e-02 1.328068e-01 15 90102276 90102502 227 - 2.522 2.436 -0.284