ENSG00000181827

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000559447 ENSG00000181827 HEK293_OSMI2_2hA HEK293_TMG_2hB RFX7 protein_coding protein_coding 3.048229 1.090166 5.26768 0.1255515 0.09618928 2.262182 1.49527798 0.08283604 2.4310092 0.08283604 0.2779200 4.716649 0.50811250 0.09306667 0.46030000 0.36723333 8.832610e-02 2.903355e-09 FALSE TRUE
ENST00000559847 ENSG00000181827 HEK293_OSMI2_2hA HEK293_TMG_2hB RFX7 protein_coding nonsense_mediated_decay 3.048229 1.090166 5.26768 0.1255515 0.09618928 2.262182 0.64129800 0.00000000 2.0789845 0.00000000 0.2301057 7.706658 0.12662500 0.00000000 0.39563333 0.39563333 2.903355e-09 2.903355e-09 TRUE TRUE
ENST00000673948 ENSG00000181827 HEK293_OSMI2_2hA HEK293_TMG_2hB RFX7 protein_coding protein_coding 3.048229 1.090166 5.26768 0.1255515 0.09618928 2.262182 0.52883108 0.00000000 0.0000000 0.00000000 0.0000000 0.000000 0.12591667 0.00000000 0.00000000 0.00000000   2.903355e-09 FALSE TRUE
ENST00000673997 ENSG00000181827 HEK293_OSMI2_2hA HEK293_TMG_2hB RFX7 protein_coding protein_coding 3.048229 1.090166 5.26768 0.1255515 0.09618928 2.262182 0.26697614 0.92177742 0.2895800 0.15766978 0.1453206 -1.637044 0.20471250 0.83456667 0.05536667 -0.77920000 2.898553e-04 2.903355e-09 FALSE TRUE
ENST00000674082 ENSG00000181827 HEK293_OSMI2_2hA HEK293_TMG_2hB RFX7 protein_coding protein_coding 3.048229 1.090166 5.26768 0.1255515 0.09618928 2.262182 0.01511245 0.08555271 0.0000000 0.04292166 0.0000000 -3.256297 0.01121250 0.07236667 0.00000000 -0.07236667 1.056526e-02 2.903355e-09 FALSE TRUE
MSTRG.10866.1 ENSG00000181827 HEK293_OSMI2_2hA HEK293_TMG_2hB RFX7 protein_coding   3.048229 1.090166 5.26768 0.1255515 0.09618928 2.262182 0.10073285 0.00000000 0.4681059 0.00000000 0.2591275 5.579258 0.02342083 0.00000000 0.08866667 0.08866667 4.768508e-01 2.903355e-09 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000181827 E001 0.0000000       15 56087280 56087280 1 -      
ENSG00000181827 E002 4.1004816 0.1621102546 1.343268e-01 2.401711e-01 15 56087281 56087509 229 - 0.520 0.950 1.774
ENSG00000181827 E003 6.7481056 0.0983887708 9.225175e-02 1.786448e-01 15 56087510 56087907 398 - 0.710 1.105 1.507
ENSG00000181827 E004 1.5028049 0.1205275262 6.659080e-01 7.734787e-01 15 56087908 56087990 83 - 0.333 0.463 0.722
ENSG00000181827 E005 330.2757021 0.0025750947 3.803802e-47 1.985176e-44 15 56087991 56093632 5642 - 2.333 2.689 1.189
ENSG00000181827 E006 263.8513660 0.0002341837 5.184390e-29 8.009128e-27 15 56093633 56096620 2988 - 2.389 2.201 -0.627
ENSG00000181827 E007 28.4944232 0.0128441583 1.262131e-03 4.850334e-03 15 56098081 56098376 296 - 1.448 1.198 -0.873
ENSG00000181827 E008 21.5686380 0.0023341579 3.380886e-05 1.985119e-04 15 56101359 56101566 208 - 1.348 0.999 -1.247
ENSG00000181827 E009 15.8756197 0.0013867865 3.770697e-06 2.760549e-05 15 56102169 56102253 85 - 1.239 0.740 -1.865
ENSG00000181827 E010 17.8857410 0.0010686205 1.890785e-07 1.820380e-06 15 56103554 56103670 117 - 1.294 0.739 -2.056
ENSG00000181827 E011 17.4973506 0.0010358876 1.663351e-06 1.316249e-05 15 56142778 56142900 123 - 1.279 0.787 -1.811
ENSG00000181827 E012 11.8007333 0.0045549686 1.282881e-05 8.311714e-05 15 56144401 56144483 83 - 1.127 0.552 -2.273
ENSG00000181827 E013 0.0000000       15 56160716 56160747 32 -      
ENSG00000181827 E014 0.0000000       15 56179059 56179133 75 -      
ENSG00000181827 E015 6.3846314 0.0028892215 7.883297e-05 4.224083e-04 15 56179270 56179303 34 - 0.900 0.215 -3.435
ENSG00000181827 E016 0.0000000       15 56189835 56189932 98 -      
ENSG00000181827 E017 9.8792257 0.0017800135 3.692607e-01 5.136819e-01 15 56243125 56243287 163 - 0.982 0.939 -0.158
ENSG00000181827 E018 3.1197754 0.0183790087 8.657593e-01 9.175394e-01 15 56243445 56243602 158 - 0.541 0.624 0.375
ENSG00000181827 E019 3.4063244 0.0045118212 8.986359e-04 3.609538e-03 15 56243603 56243879 277 - 0.681 0.000 -12.242
ENSG00000181827 E020 2.0444483 0.2007951179 6.653143e-01 7.730018e-01 15 56243915 56244085 171 - 0.451 0.362 -0.485
ENSG00000181827 E021 0.7490443 0.0743536702 2.328568e-01 3.658203e-01 15 56244297 56244409 113 - 0.262 0.000 -9.779
ENSG00000181827 E022 0.0000000       15 56244840 56245082 243 -