ENSG00000181744

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000315691 ENSG00000181744 HEK293_OSMI2_2hA HEK293_TMG_2hB DIPK2A protein_coding protein_coding 6.824176 2.270793 11.0813 0.3811643 0.277157 2.281822 3.300293 0.5087044 6.323535 0.01695577 0.1159532 3.610027 0.3850500 0.2403000 0.5715333 0.3312333 3.191828e-05 6.070211e-06 FALSE TRUE
ENST00000441925 ENSG00000181744 HEK293_OSMI2_2hA HEK293_TMG_2hB DIPK2A protein_coding protein_coding 6.824176 2.270793 11.0813 0.3811643 0.277157 2.281822 3.354132 1.7267721 4.541186 0.36719772 0.3833483 1.389834 0.5803958 0.7476667 0.4089000 -0.3387667 6.070211e-06 6.070211e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000181744 E001 49.4238053 0.0044131384 2.220260e-03 7.906570e-03 3 143971823 143972636 814 + 1.634 1.417 -0.743
ENSG00000181744 E002 9.5867841 0.0027862756 1.152045e-02 3.239547e-02 3 143972637 143972654 18 + 0.983 0.597 -1.543
ENSG00000181744 E003 33.3224034 0.0006791060 1.221392e-04 6.235893e-04 3 143972655 143972876 222 + 1.481 1.155 -1.138
ENSG00000181744 E004 36.0102773 0.0006254095 6.373271e-07 5.521296e-06 3 143972877 143972989 113 + 1.522 1.082 -1.543
ENSG00000181744 E005 0.0000000       3 143973081 143973089 9 +      
ENSG00000181744 E006 0.0000000       3 143973090 143973255 166 +      
ENSG00000181744 E007 0.1482932 0.0411597534 7.922104e-02   3 143973256 143973562 307 + 0.000 0.240 12.813
ENSG00000181744 E008 0.2214452 0.0411540662 7.797701e-02   3 143973608 143973705 98 + 0.000 0.241 12.819
ENSG00000181744 E009 0.2924217 0.0273730156 1.000000e+00   3 143978462 143978522 61 + 0.110 0.000 -11.954
ENSG00000181744 E010 77.0002640 0.0003421836 1.997421e-06 1.554026e-05 3 143985543 143985846 304 + 1.827 1.579 -0.841
ENSG00000181744 E011 0.4407149 0.0212115476 4.606625e-01 6.006596e-01 3 143985847 143986155 309 + 0.110 0.240 1.363
ENSG00000181744 E012 478.6528367 0.0007789261 7.277022e-20 4.537887e-18 3 143989510 143992368 2859 + 2.553 2.669 0.387
ENSG00000181744 E013 0.0000000       3 144034550 144034680 131 +      
ENSG00000181744 E014 0.0000000       3 144048668 144048719 52 +