ENSG00000181690

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000316981 ENSG00000181690 HEK293_OSMI2_2hA HEK293_TMG_2hB PLAG1 protein_coding protein_coding 2.374966 0.2816197 4.163898 0.0376991 0.4217555 3.839235 1.5787088 0.275104168 2.568032 0.033080906 0.08246802 3.176709 0.7990583 0.98073333 0.6271000 -0.3536333 0.006819781 0.006819781 FALSE TRUE
ENST00000423799 ENSG00000181690 HEK293_OSMI2_2hA HEK293_TMG_2hB PLAG1 protein_coding protein_coding 2.374966 0.2816197 4.163898 0.0376991 0.4217555 3.839235 0.6357577 0.006515567 1.245211 0.006515567 0.25468393 6.247960 0.1601125 0.01926667 0.2927333 0.2734667 0.018350383 0.006819781 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000181690 E001 198.4240311 0.0034910054 9.121851e-12 1.913316e-10 8 56160909 56166033 5125 - 2.064 2.251 0.625
ENSG00000181690 E002 6.7968403 0.0025006271 1.983361e-01 3.241367e-01 8 56166034 56166098 65 - 0.728 0.491 -1.049
ENSG00000181690 E003 8.3950623 0.0019695887 2.483474e-01 3.839774e-01 8 56166099 56166242 144 - 0.803 0.618 -0.766
ENSG00000181690 E004 52.3343658 0.0007060586 2.336311e-06 1.791134e-05 8 56166243 56167341 1099 - 1.548 1.133 -1.448
ENSG00000181690 E005 19.1218478 0.0128015923 6.524539e-03 1.998665e-02 8 56167342 56167503 162 - 1.134 0.715 -1.588
ENSG00000181690 E006 9.3777462 0.0019074503 1.549216e-01 2.683132e-01 8 56168028 56168386 359 - 0.849 0.618 -0.945
ENSG00000181690 E007 10.2679243 0.0017825449 1.020261e-01 1.936030e-01 8 56171091 56171189 99 - 0.884 0.618 -1.083
ENSG00000181690 E008 0.0000000       8 56171190 56171424 235 -      
ENSG00000181690 E009 1.7757486 0.0077645399 2.644624e-01 4.024323e-01 8 56179409 56179513 105 - 0.345 0.001 -9.627
ENSG00000181690 E010 0.1451727 0.0440627523 5.968448e-01   8 56209253 56209483 231 - 0.042 0.001 -6.225
ENSG00000181690 E011 5.4693099 0.0030909137 1.000116e-01 1.905033e-01 8 56211121 56211324 204 - 0.666 0.311 -1.793