ENSG00000181450

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000343776 ENSG00000181450 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF678 protein_coding protein_coding 6.17775 1.841905 9.609151 0.1165209 0.6575984 2.376899 1.0764208 0.1197301 2.8414755 0.11973012 0.5299643 4.4581232 0.1483583 0.07313333 0.3048667 0.2317333 1.543121e-01 1.17005e-09 FALSE TRUE
ENST00000397097 ENSG00000181450 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF678 protein_coding protein_coding 6.17775 1.841905 9.609151 0.1165209 0.6575984 2.376899 1.0537415 0.5001909 0.7502967 0.29956709 0.4036514 0.5755253 0.1946167 0.25303333 0.0736000 -0.1794333 7.639492e-01 1.17005e-09 FALSE TRUE
ENST00000440339 ENSG00000181450 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF678 protein_coding protein_coding 6.17775 1.841905 9.609151 0.1165209 0.6575984 2.376899 1.6582088 0.0000000 3.0723766 0.00000000 0.6491697 8.2678993 0.1708167 0.00000000 0.3153000 0.3153000 1.170050e-09 1.17005e-09 FALSE TRUE
ENST00000465266 ENSG00000181450 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF678 protein_coding processed_transcript 6.17775 1.841905 9.609151 0.1165209 0.6575984 2.376899 0.7537435 0.0000000 1.2904142 0.00000000 0.3747381 7.0228275 0.1104458 0.00000000 0.1325000 0.1325000 1.109924e-04 1.17005e-09   FALSE
ENST00000608949 ENSG00000181450 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF678 protein_coding protein_coding 6.17775 1.841905 9.609151 0.1165209 0.6575984 2.376899 0.7595883 0.6789952 1.1057271 0.20793194 0.1316876 0.6954182 0.1722042 0.38440000 0.1157333 -0.2686667 8.797945e-02 1.17005e-09   FALSE
MSTRG.3174.3 ENSG00000181450 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF678 protein_coding   6.17775 1.841905 9.609151 0.1165209 0.6575984 2.376899 0.1977484 0.4010314 0.0000000 0.21377674 0.0000000 -5.3611765 0.0928750 0.20896667 0.0000000 -0.2089667 3.086171e-02 1.17005e-09 FALSE TRUE
MSTRG.3174.4 ENSG00000181450 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF678 protein_coding   6.17775 1.841905 9.609151 0.1165209 0.6575984 2.376899 0.6782984 0.1419575 0.5488606 0.05428043 0.3378315 1.8788207 0.1106792 0.08050000 0.0580000 -0.0225000 8.576715e-01 1.17005e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000181450 E001 2.889233 0.0052289795 8.954124e-01 9.372051e-01 1 227563543 227563550 8 + 0.488 0.538 0.238
ENSG00000181450 E002 3.512684 0.0046730076 9.501727e-01 9.728287e-01 1 227563551 227563554 4 + 0.540 0.630 0.405
ENSG00000181450 E003 3.512684 0.0046730076 9.501727e-01 9.728287e-01 1 227563555 227563555 1 + 0.540 0.630 0.405
ENSG00000181450 E004 9.159440 0.0019121334 4.884458e-01 6.260078e-01 1 227563556 227563579 24 + 0.851 1.033 0.684
ENSG00000181450 E005 26.881773 0.0495647427 1.580205e-01 2.724970e-01 1 227563580 227563724 145 + 1.265 1.518 0.879
ENSG00000181450 E006 20.939733 0.0054882885 1.238677e-03 4.771721e-03 1 227582514 227582606 93 + 1.125 1.506 1.331
ENSG00000181450 E007 27.430301 0.0008021709 5.536051e-01 6.827092e-01 1 227646544 227646670 127 + 1.297 1.446 0.518
ENSG00000181450 E008 11.942190 0.0015218500 1.561423e-02 4.187587e-02 1 227650956 227650991 36 + 1.020 0.771 -0.951
ENSG00000181450 E009 15.252330 0.0098360902 3.200530e-02 7.586865e-02 1 227650992 227651076 85 + 1.112 0.921 -0.701
ENSG00000181450 E010 31.100222 0.0297317205 1.142403e-01 2.117245e-01 1 227654336 227654476 141 + 1.392 1.281 -0.386
ENSG00000181450 E011 130.978025 0.0082084792 1.362307e-13 3.797601e-12 1 227654477 227655763 1287 + 2.030 1.654 -1.268
ENSG00000181450 E012 347.093270 0.0963090915 4.995713e-01 6.358740e-01 1 227655764 227662462 6699 + 2.378 2.542 0.548
ENSG00000181450 E013 10.993650 0.0927185330 3.951343e-05 2.280627e-04 1 227677179 227677443 265 + 0.711 1.466 2.767