ENSG00000181315

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000415922 ENSG00000181315 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF322 protein_coding protein_coding 15.9306 4.941428 27.71606 0.3305264 0.8113623 2.485326 11.934546 3.284379 20.181959 0.2017792 0.9517212 2.615703 0.7325083 0.6660333 0.7301 0.06406667 0.62398860 0.001010443 FALSE TRUE
MSTRG.27815.11 ENSG00000181315 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF322 protein_coding   15.9306 4.941428 27.71606 0.3305264 0.8113623 2.485326 1.512764 1.531851 4.038842 0.1753657 0.7726652 1.392846 0.1682000 0.3101333 0.1453 -0.16483333 0.01139288 0.001010443   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000181315 E001 0.1515154 0.0431927647 8.773357e-01   6 26634383 26634392 10 - 0.053 0.000 -7.076
ENSG00000181315 E002 0.1515154 0.0431927647 8.773357e-01   6 26634393 26634533 141 - 0.053 0.000 -8.878
ENSG00000181315 E003 46.3609232 0.0006304646 4.624884e-15 1.600019e-13 6 26634534 26635086 553 - 1.442 1.882 1.495
ENSG00000181315 E004 24.0422641 0.0013809829 2.448508e-10 4.038687e-09 6 26635087 26635250 164 - 1.153 1.638 1.685
ENSG00000181315 E005 3.0915731 0.0115430104 4.464297e-03 1.444104e-02 6 26635251 26635565 315 - 0.407 0.877 2.071
ENSG00000181315 E006 2.3562318 0.0064516380 1.562649e-04 7.749918e-04 6 26635566 26635631 66 - 0.250 0.878 3.075
ENSG00000181315 E007 86.5657886 0.0003519103 8.733584e-01 9.226242e-01 6 26635632 26636290 659 - 1.811 1.817 0.020
ENSG00000181315 E008 205.0664123 0.0004821394 1.180801e-01 2.171816e-01 6 26636291 26638186 1896 - 2.189 2.138 -0.170
ENSG00000181315 E009 94.2672791 0.0036190237 1.047725e-06 8.656272e-06 6 26638187 26638475 289 - 1.884 1.568 -1.067
ENSG00000181315 E010 28.1268228 0.0060898197 1.087451e-03 4.260985e-03 6 26638476 26638626 151 - 1.379 1.000 -1.350
ENSG00000181315 E011 9.6528032 0.0025267600 5.356063e-02 1.154842e-01 6 26638627 26638639 13 - 0.949 0.630 -1.272
ENSG00000181315 E012 29.0224457 0.0007332046 1.938562e-03 7.036180e-03 6 26638640 26638728 89 - 1.388 1.066 -1.141
ENSG00000181315 E013 38.0389666 0.0005937468 8.209640e-03 2.431511e-02 6 26643659 26643728 70 - 1.490 1.259 -0.800
ENSG00000181315 E014 0.2934659 0.0285317647 1.805493e-01   6 26650406 26650444 39 - 0.053 0.259 2.657
ENSG00000181315 E015 0.0000000       6 26651369 26651496 128 -      
ENSG00000181315 E016 11.4775395 0.0015545578 8.669952e-22 6.731542e-20 6 26654439 26654566 128 - 0.627 1.568 3.474
ENSG00000181315 E017 72.2625016 0.0026998567 5.547405e-01 6.836902e-01 6 26658558 26658646 89 - 1.738 1.700 -0.130
ENSG00000181315 E018 1.6410832 0.0782720717 7.185950e-01 8.136457e-01 6 26658777 26658851 75 - 0.336 0.418 0.473
ENSG00000181315 E019 94.1833040 0.0064069559 3.017446e-02 7.231175e-02 6 26659441 26659752 312 - 1.866 1.727 -0.470