ENSG00000180694

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000422900 ENSG00000180694 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM64 protein_coding protein_coding 4.804898 2.018428 8.340085 0.9422185 0.1958787 2.041428 1.036846 0.000000 1.578849 0.0000000 0.2081043 7.311838 0.143075 0.0000000 0.1888333 0.1888333 1.833078e-08 1.833078e-08 FALSE TRUE
ENST00000458549 ENSG00000180694 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM64 protein_coding protein_coding 4.804898 2.018428 8.340085 0.9422185 0.1958787 2.041428 3.709720 1.997899 6.664814 0.9533668 0.1791905 1.733041 0.831625 0.9796667 0.7996000 -0.1800667 4.406355e-06 1.833078e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000180694 E001 0.0000000       8 90621995 90621999 5 -      
ENSG00000180694 E002 307.3848272 0.0003469175 1.614965e-49 9.501564e-47 8 90622000 90625090 3091 - 2.387 2.603 0.719
ENSG00000180694 E003 154.0404507 0.0007061276 2.213462e-04 1.055273e-03 8 90625091 90625670 580 - 2.192 2.042 -0.501
ENSG00000180694 E004 85.0290583 0.0003650842 3.526557e-08 3.915005e-07 8 90625671 90625862 192 - 1.961 1.680 -0.950
ENSG00000180694 E005 30.6441377 0.0006520420 2.320347e-02 5.816896e-02 8 90631552 90631565 14 - 1.512 1.317 -0.675
ENSG00000180694 E006 69.3523611 0.0003951385 1.801719e-06 1.415545e-05 8 90631566 90631707 142 - 1.872 1.601 -0.918
ENSG00000180694 E007 111.4121702 0.0589928887 1.080647e-02 3.070080e-02 8 90645111 90646083 973 - 2.089 1.708 -1.284
ENSG00000180694 E008 0.0000000       8 90683816 90683950 135 -      
ENSG00000180694 E009 0.0000000       8 90683951 90684030 80 -      
ENSG00000180694 E010 0.0000000       8 90690444 90690575 132 -      
ENSG00000180694 E011 0.0000000       8 90703394 90703522 129 -      
ENSG00000180694 E012 0.0000000       8 90709436 90709556 121 -      
ENSG00000180694 E013 0.0000000       8 90727499 90727579 81 -      
ENSG00000180694 E014 0.1817044 0.3732876111 1.369759e-01   8 90791438 90791632 195 - 0.000 0.195 10.236