ENSG00000180228

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000325748 ENSG00000180228 HEK293_OSMI2_2hA HEK293_TMG_2hB PRKRA protein_coding protein_coding 33.13885 31.28866 44.83524 1.526274 1.406185 0.5188543 22.456423 24.3624357 28.365586 1.5808267 1.0916703 0.2193997 0.6784083 0.77800000 0.6324333 -0.1455667 7.279740e-04 6.428725e-11 FALSE TRUE
ENST00000466165 ENSG00000180228 HEK293_OSMI2_2hA HEK293_TMG_2hB PRKRA protein_coding retained_intron 33.13885 31.28866 44.83524 1.526274 1.406185 0.5188543 2.531384 0.3749477 5.319739 0.0703186 0.5694675 3.7913306 0.0714125 0.01223333 0.1193000 0.1070667 6.428725e-11 6.428725e-11 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000180228 E001 0.2955422 0.0293863233 9.254226e-01   2 178431292 178431413 122 - 0.115 0.130 0.200
ENSG00000180228 E002 0.5169874 0.0202761648 4.907199e-01 6.280598e-01 2 178431414 178431414 1 - 0.115 0.230 1.203
ENSG00000180228 E003 4.5069079 0.0619799573 5.414447e-03 1.705406e-02 2 178431415 178431434 20 - 0.401 0.907 2.220
ENSG00000180228 E004 9.5986894 0.0087335274 1.331952e-02 3.666434e-02 2 178431435 178431439 5 - 0.849 1.133 1.053
ENSG00000180228 E005 186.2785326 0.0002431823 1.339694e-06 1.082630e-05 2 178431440 178431653 214 - 2.204 2.331 0.426
ENSG00000180228 E006 483.3623660 0.0002136653 2.321205e-15 8.366538e-14 2 178431654 178431965 312 - 2.616 2.746 0.435
ENSG00000180228 E007 173.0380644 0.0002129764 6.623271e-06 4.586442e-05 2 178431966 178431975 10 - 2.175 2.298 0.410
ENSG00000180228 E008 465.3189690 0.0005681059 7.558876e-12 1.605028e-10 2 178431976 178432254 279 - 2.600 2.729 0.431
ENSG00000180228 E009 288.4276525 0.0001606186 1.241752e-04 6.329947e-04 2 178436145 178436319 175 - 2.421 2.501 0.268
ENSG00000180228 E010 18.4282290 0.0070227366 3.432001e-09 4.607954e-08 2 178436320 178438827 2508 - 1.488 0.921 -2.018
ENSG00000180228 E011 0.2987644 0.0275182126 2.430740e-01   2 178440139 178440257 119 - 0.206 0.000 -9.146
ENSG00000180228 E012 9.0058403 0.0018582181 1.347451e-09 1.949143e-08 2 178440258 178441609 1352 - 1.240 0.491 -2.968
ENSG00000180228 E013 265.7769627 0.0021118354 5.257126e-01 6.590536e-01 2 178441610 178441704 95 - 2.417 2.434 0.055
ENSG00000180228 E014 2.5454169 0.0059790887 2.319450e-03 8.212251e-03 2 178441705 178443225 1521 - 0.744 0.230 -2.705
ENSG00000180228 E015 4.1902027 0.0041528070 3.326400e-02 7.831878e-02 2 178443226 178443266 41 - 0.535 0.841 1.290
ENSG00000180228 E016 181.4336664 0.0027631077 3.198483e-01 4.629226e-01 2 178443267 178443289 23 - 2.244 2.277 0.110
ENSG00000180228 E017 268.5239298 0.0016277642 5.674167e-01 6.942263e-01 2 178443290 178443384 95 - 2.423 2.437 0.046
ENSG00000180228 E018 1.5447463 0.0088467201 2.291482e-01 3.614156e-01 2 178443385 178443403 19 - 0.281 0.491 1.203
ENSG00000180228 E019 3.1890387 0.0062035371 3.029347e-01 4.448498e-01 2 178443404 178444421 1018 - 0.694 0.537 -0.691
ENSG00000180228 E020 281.0293381 0.0001706727 4.837203e-01 6.217445e-01 2 178444422 178444500 79 - 2.443 2.459 0.052
ENSG00000180228 E021 47.3110474 0.0028121903 6.233330e-14 1.823762e-12 2 178444501 178445425 925 - 1.854 1.406 -1.524
ENSG00000180228 E022 28.1426180 0.0007722174 2.604686e-01 3.979981e-01 2 178445426 178445494 69 - 1.496 1.423 -0.251
ENSG00000180228 E023 22.6652039 0.0231500860 2.997011e-01 4.412678e-01 2 178445495 178445535 41 - 1.419 1.311 -0.373
ENSG00000180228 E024 76.5095130 0.0003510601 6.935850e-34 1.566121e-31 2 178445536 178446954 1419 - 2.083 1.551 -1.798
ENSG00000180228 E025 16.8139145 0.0265606971 4.962208e-08 5.353809e-07 2 178447320 178447455 136 - 1.475 0.795 -2.460
ENSG00000180228 E026 19.1999503 0.0044618341 1.970589e-05 1.224168e-04 2 178447456 178447504 49 - 1.452 1.086 -1.288
ENSG00000180228 E027 149.0218186 0.0002329917 4.825174e-01 6.206520e-01 2 178447505 178447515 11 - 2.188 2.169 -0.064
ENSG00000180228 E028 260.6769201 0.0001645951 4.244903e-03 1.382710e-02 2 178447516 178447586 71 - 2.449 2.388 -0.205
ENSG00000180228 E029 3.0740576 0.0051085441 5.000958e-02 1.091921e-01 2 178450079 178450241 163 - 0.744 0.438 -1.383
ENSG00000180228 E030 278.4644312 0.0001720147 2.986831e-05 1.778328e-04 2 178450242 178450365 124 - 2.491 2.404 -0.290
ENSG00000180228 E031 174.0668655 0.0002509666 2.459036e-05 1.494425e-04 2 178450366 178450411 46 - 2.300 2.187 -0.377
ENSG00000180228 E032 10.1660021 0.0264246097 1.939880e-01 3.188068e-01 2 178450412 178450561 150 - 1.128 0.956 -0.630
ENSG00000180228 E033 7.5914070 0.0022916198 2.507519e-03 8.785847e-03 2 178450562 178450965 404 - 1.076 0.715 -1.382
ENSG00000180228 E034 157.0936416 0.0014240931 6.920192e-06 4.771645e-05 2 178450966 178451021 56 - 2.270 2.122 -0.495
ENSG00000180228 E035 145.8832953 0.0007422203 3.181959e-07 2.928549e-06 2 178451022 178451278 257 - 2.243 2.083 -0.535
ENSG00000180228 E036 2.3217910 0.0062641777 6.107036e-01 7.299890e-01 2 178451381 178452721 1341 - 0.572 0.490 -0.384