ENSG00000179526

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000359551 ENSG00000179526 HEK293_OSMI2_2hA HEK293_TMG_2hB SHARPIN protein_coding nonsense_mediated_decay 48.30082 78.55797 29.8658 1.563848 0.9333906 -1.394964 6.083445 5.667311 4.879557 1.1220009 0.1600700 -0.2155041 0.12584583 0.07203333 0.163400000 0.091366667 5.857849e-03 7.957515e-10 TRUE  
ENST00000398712 ENSG00000179526 HEK293_OSMI2_2hA HEK293_TMG_2hB SHARPIN protein_coding protein_coding 48.30082 78.55797 29.8658 1.563848 0.9333906 -1.394964 18.246061 30.123951 12.510678 2.0144145 0.7616020 -1.2670772 0.35789583 0.38376667 0.418433333 0.034666667 7.042265e-01 7.957515e-10 FALSE  
ENST00000525275 ENSG00000179526 HEK293_OSMI2_2hA HEK293_TMG_2hB SHARPIN protein_coding retained_intron 48.30082 78.55797 29.8658 1.563848 0.9333906 -1.394964 3.144087 3.941869 1.554420 0.4922556 0.5301257 -1.3369074 0.06999167 0.05013333 0.051366667 0.001233333 9.833848e-01 7.957515e-10 FALSE  
ENST00000530216 ENSG00000179526 HEK293_OSMI2_2hA HEK293_TMG_2hB SHARPIN protein_coding retained_intron 48.30082 78.55797 29.8658 1.563848 0.9333906 -1.394964 4.840150 7.380585 2.858279 1.2945337 0.6019353 -1.3655031 0.10447083 0.09376667 0.095133333 0.001366667 1.000000e+00 7.957515e-10    
ENST00000532536 ENSG00000179526 HEK293_OSMI2_2hA HEK293_TMG_2hB SHARPIN protein_coding protein_coding 48.30082 78.55797 29.8658 1.563848 0.9333906 -1.394964 3.604569 6.418451 2.378919 0.8066037 0.1683414 -1.4281134 0.07592083 0.08186667 0.079966667 -0.001900000 9.950530e-01 7.957515e-10 FALSE  
ENST00000534435 ENSG00000179526 HEK293_OSMI2_2hA HEK293_TMG_2hB SHARPIN protein_coding retained_intron 48.30082 78.55797 29.8658 1.563848 0.9333906 -1.394964 2.382520 3.248417 1.922099 0.1526608 0.3790022 -0.7540019 0.05609167 0.04130000 0.065266667 0.023966667 4.225340e-01 7.957515e-10    
MSTRG.32246.17 ENSG00000179526 HEK293_OSMI2_2hA HEK293_TMG_2hB SHARPIN protein_coding   48.30082 78.55797 29.8658 1.563848 0.9333906 -1.394964 2.154718 6.248786 0.000000 2.1367861 0.0000000 -9.2897390 0.04745417 0.07920000 0.000000000 -0.079200000 7.957515e-10 7.957515e-10 FALSE  
MSTRG.32246.8 ENSG00000179526 HEK293_OSMI2_2hA HEK293_TMG_2hB SHARPIN protein_coding   48.30082 78.55797 29.8658 1.563848 0.9333906 -1.394964 2.006505 7.689147 0.150502 0.5254678 0.1505020 -5.5840352 0.03817083 0.09820000 0.004766667 -0.093433333 8.345202e-03 7.957515e-10    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000179526 E001 1.624139 0.0091320969 3.252447e-01 0.4686780452 8 144098491 144098627 137 - 0.199 0.391 1.328
ENSG00000179526 E002 0.772888 0.0153268335 2.943159e-01 0.4354267279 8 144098628 144098632 5 - 0.001 0.238 9.008
ENSG00000179526 E003 1.139506 0.0220845955 6.606031e-01 0.7692405000 8 144098633 144098635 3 - 0.199 0.282 0.654
ENSG00000179526 E004 1.648087 0.0586839218 8.806511e-01 0.9274479958 8 144098636 144098636 1 - 0.335 0.360 0.150
ENSG00000179526 E005 3.754672 0.0041679834 8.765224e-01 0.9247497324 8 144098637 144098637 1 - 0.592 0.612 0.092
ENSG00000179526 E006 7.107239 0.0023642024 7.628726e-01 0.8463817566 8 144098638 144098638 1 - 0.869 0.823 -0.179
ENSG00000179526 E007 7.884294 0.0021967100 5.844673e-01 0.7083791961 8 144098639 144098639 1 - 0.932 0.857 -0.287
ENSG00000179526 E008 220.949970 0.0016145533 9.886631e-01 0.9969673553 8 144098640 144098788 149 - 2.261 2.255 -0.019
ENSG00000179526 E009 26.818354 0.0026340155 6.846698e-01 0.7876070654 8 144098789 144098865 77 - 1.341 1.368 0.096
ENSG00000179526 E010 383.605825 0.0010542571 1.234761e-01 0.2249075342 8 144098866 144098989 124 - 2.466 2.501 0.114
ENSG00000179526 E011 199.762581 0.0004253368 5.917786e-01 0.7144630170 8 144098990 144098994 5 - 2.205 2.215 0.033
ENSG00000179526 E012 214.479580 0.0001895777 8.640814e-01 0.9163445450 8 144099081 144099121 41 - 2.242 2.240 -0.007
ENSG00000179526 E013 257.213132 0.0012733554 2.152742e-01 0.3447368663 8 144099122 144099199 78 - 2.293 2.326 0.110
ENSG00000179526 E014 125.959394 0.0013195804 1.477757e-01 0.2586562968 8 144099200 144099205 6 - 1.968 2.022 0.183
ENSG00000179526 E015 19.884240 0.0489841118 4.605071e-01 0.6005402367 8 144099250 144099276 27 - 1.329 1.207 -0.426
ENSG00000179526 E016 240.331682 0.0015179912 1.033723e-01 0.1956205768 8 144099277 144099290 14 - 2.252 2.301 0.161
ENSG00000179526 E017 216.943171 0.0014659533 1.789769e-02 0.0469179188 8 144099291 144099300 10 - 2.185 2.262 0.255
ENSG00000179526 E018 360.732335 0.0001951074 1.671445e-05 0.0001055184 8 144099301 144099402 102 - 2.391 2.485 0.311
ENSG00000179526 E019 226.478999 0.0002121972 5.944667e-02 0.1256904964 8 144099403 144099430 28 - 2.228 2.275 0.157
ENSG00000179526 E020 19.460259 0.0027621505 8.145945e-01 0.8825467756 8 144099431 144099509 79 - 1.237 1.208 -0.103
ENSG00000179526 E021 187.902419 0.0002199239 1.954187e-02 0.0505036696 8 144099510 144099518 9 - 2.131 2.198 0.225
ENSG00000179526 E022 327.918828 0.0002231096 6.188025e-03 0.0191090639 8 144099519 144099618 100 - 2.377 2.436 0.198
ENSG00000179526 E023 499.579327 0.0014261603 3.010429e-01 0.4428158119 8 144099703 144099844 142 - 2.592 2.610 0.060
ENSG00000179526 E024 553.531096 0.0001140120 8.836405e-02 0.1726231704 8 144099929 144100069 141 - 2.680 2.643 -0.122
ENSG00000179526 E025 23.462791 0.0303063575 7.720734e-01 0.8528885924 8 144100743 144100842 100 - 1.279 1.310 0.108
ENSG00000179526 E026 37.272875 0.0140850820 5.695845e-02 0.1213873471 8 144102642 144102816 175 - 1.613 1.449 -0.565
ENSG00000179526 E027 35.342390 0.0006225817 1.688283e-03 0.0062455376 8 144102817 144103050 234 - 1.626 1.418 -0.714
ENSG00000179526 E028 366.037050 0.0004273377 9.095853e-03 0.0265218415 8 144103051 144103116 66 - 2.522 2.456 -0.222
ENSG00000179526 E029 322.498068 0.0009284806 7.440887e-03 0.0223640967 8 144103117 144103225 109 - 2.475 2.398 -0.258
ENSG00000179526 E030 158.985802 0.0094849326 7.512551e-05 0.0004048213 8 144103553 144103846 294 - 2.277 2.053 -0.750
ENSG00000179526 E031 88.428592 0.0098995920 5.214081e-03 0.0165132908 8 144103847 144104024 178 - 1.994 1.812 -0.612
ENSG00000179526 E032 96.725976 0.0057984206 3.341391e-03 0.0112666444 8 144104025 144104264 240 - 2.021 1.856 -0.555
ENSG00000179526 E033 15.606694 0.0396157837 4.849492e-02 0.1064982710 8 144104265 144104412 148 - 1.328 1.061 -0.949
ENSG00000179526 E034 0.000000       8 144105727 144105862 136 -      
ENSG00000179526 E035 0.000000       8 144108018 144108124 107 -