ENSG00000179454

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000355081 ENSG00000179454 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL28 protein_coding protein_coding 3.222087 1.756136 4.422 0.3618751 0.01492655 1.327362 0.5711013 0.11276198 0.50621108 0.11276198 0.13201978 2.0720973 0.15090417 0.0961000 0.11460000 0.01850000 5.376082e-01 2.508443e-06 FALSE TRUE
ENST00000396128 ENSG00000179454 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL28 protein_coding protein_coding 3.222087 1.756136 4.422 0.3618751 0.01492655 1.327362 1.4623836 0.36127524 2.52013478 0.06165780 0.09701421 2.7686532 0.39148750 0.2134333 0.56980000 0.35636667 2.508443e-06 2.508443e-06 FALSE TRUE
ENST00000556500 ENSG00000179454 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL28 protein_coding protein_coding 3.222087 1.756136 4.422 0.3618751 0.01492655 1.327362 0.1998928 0.08028938 0.26227035 0.08028938 0.16264131 1.5924116 0.05612917 0.0332000 0.05923333 0.02603333 8.362446e-01 2.508443e-06 FALSE FALSE
ENST00000579157 ENSG00000179454 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL28 protein_coding protein_coding 3.222087 1.756136 4.422 0.3618751 0.01492655 1.327362 0.1044369 0.18030759 0.12232274 0.10487739 0.08305379 -0.5242721 0.05347083 0.0854000 0.02780000 -0.05760000 5.597086e-01 2.508443e-06 FALSE FALSE
MSTRG.9456.3 ENSG00000179454 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL28 protein_coding   3.222087 1.756136 4.422 0.3618751 0.01492655 1.327362 0.1875339 0.04180044 0.57024800 0.02107487 0.16570978 3.4856334 0.04204583 0.0214000 0.12883333 0.10743333 7.895409e-02 2.508443e-06 FALSE TRUE
MSTRG.9456.5 ENSG00000179454 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL28 protein_coding   3.222087 1.756136 4.422 0.3618751 0.01492655 1.327362 0.2122123 0.30304553 0.09548672 0.11600188 0.04776799 -1.5693111 0.10555833 0.1757333 0.02160000 -0.15413333 4.147993e-02 2.508443e-06   FALSE
MSTRG.9456.6 ENSG00000179454 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL28 protein_coding   3.222087 1.756136 4.422 0.3618751 0.01492655 1.327362 0.4559297 0.67665541 0.26743866 0.22064523 0.06622650 -1.3074175 0.18595833 0.3747000 0.06053333 -0.31416667 2.033123e-05 2.508443e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000179454 E001 145.3615924 0.0003407360 1.362941e-08 1.638565e-07 14 44924324 44928812 4489 - 2.133 1.950 -0.613
ENSG00000179454 E002 6.9107018 0.0023797981 1.014798e-01 1.927206e-01 14 44928813 44928938 126 - 0.880 0.625 -1.030
ENSG00000179454 E003 12.0094811 0.0018874902 1.367709e-05 8.802727e-05 14 44928939 44929191 253 - 1.136 0.467 -2.713
ENSG00000179454 E004 14.1554085 0.0047902418 1.114557e-06 9.162087e-06 14 44931333 44931541 209 - 1.208 0.467 -2.969
ENSG00000179454 E005 34.5974334 0.0006657194 1.845496e-05 1.154293e-04 14 44934115 44934558 444 - 1.543 1.199 -1.194
ENSG00000179454 E006 6.4430895 0.0030389172 6.829021e-13 1.721999e-11 14 44937703 44937812 110 - 0.394 1.280 3.607
ENSG00000179454 E007 17.6834899 0.0011340757 3.115833e-03 1.060691e-02 14 44945030 44945115 86 - 1.130 1.383 0.891
ENSG00000179454 E008 20.9545573 0.0010112702 4.655652e-02 1.030448e-01 14 44945116 44945232 117 - 1.233 1.395 0.563
ENSG00000179454 E009 29.3857758 0.0007561165 5.040944e-04 2.175426e-03 14 44945233 44945440 208 - 1.350 1.584 0.804
ENSG00000179454 E010 14.6307455 0.0012830143 6.257075e-01 7.417830e-01 14 44945441 44945495 55 - 1.114 1.162 0.172
ENSG00000179454 E011 13.0510698 0.0084203263 1.049060e-01 1.979574e-01 14 44945496 44945525 30 - 1.029 1.200 0.615
ENSG00000179454 E012 26.7507377 0.0008021709 3.536205e-02 8.234670e-02 14 44945526 44945676 151 - 1.334 1.486 0.527
ENSG00000179454 E013 24.8428345 0.0008047902 2.848752e-01 4.251600e-01 14 44945677 44945791 115 - 1.324 1.406 0.286
ENSG00000179454 E014 15.7964299 0.0011680827 5.801448e-01 7.049685e-01 14 44945792 44945808 17 - 1.146 1.199 0.189
ENSG00000179454 E015 32.3185887 0.0007294658 2.473348e-02 6.132853e-02 14 44945809 44945928 120 - 1.412 1.561 0.512
ENSG00000179454 E016 1.2522995 0.1012470916 5.384846e-02 1.159854e-01 14 44953623 44953709 87 - 0.174 0.558 2.406
ENSG00000179454 E017 0.0000000       14 44959341 44959514 174 -      
ENSG00000179454 E018 0.8793415 0.1933619732 2.787720e-01 4.184363e-01 14 44960887 44961132 246 - 0.298 0.000 -10.025
ENSG00000179454 E019 2.5017087 0.7327986119 8.798099e-01 9.268813e-01 14 44961597 44961845 249 - 0.447 0.589 0.679
ENSG00000179454 E020 21.0335469 0.0010112702 5.975295e-02 1.261986e-01 14 44961846 44962190 345 - 1.229 1.384 0.539
ENSG00000179454 E021 0.0000000       14 44963851 44964092 242 -      
ENSG00000179454 E022 0.0000000       14 44978198 44978298 101 -      
ENSG00000179454 E023 0.0000000       14 45042116 45042322 207 -